<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_sjph-2016-0018_s_379_w2aab3b8c13b1b7b1aab1c15b1Aa"><h3>Introduction</h3><p>According to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant <italic>Staphylococcus aureus</italic> (CA-MRSA) circulate in Europe. In Europe, the European clone that belongs to sequence type ST80 is predominant.</p></sec><sec id="j_sjph-2016-0018_s_380_w2aab3b8c13b1b7b1aab1c15b2Aa"><h3>Methods</h3><p>The aim of the study was to investigate the phenotypic and genotypic characteristics and epidemiological data of CA-MRSA ST80 and its occurrence in Slovenia. We retrospectively analyzed those CA-MRSA isolates that were isolated during microbiological procedures in microbiological laboratories between 2006 and 2013. Only CA-MRSA isolates from the national collection of CA-MRSA strains that belonged to ST80 (European clone) were analyzed. We determined the Pantone-Valentine leukocidin (PVL), <italic>mec</italic> A genes, exfoliative toxin genes and type of staphylococcal cassette chromosome (SCC<italic>mec</italic>) by polymerase chain reaction (PCR). We determined also spa type and sequence type.</p></sec><sec id="j_sjph-2016-0018_s_382_w2aab3b8c13b1b7b1aab1c15b3Aa"><h3>Results</h3><p>ST80 was confirmed in only 2 (0.5%) out of 385 CA-MRSA isolates, collected in a national collection of CAMRSA. Both isolates were positive for the PVL genes, <italic>mec</italic> A gene, exfoliative toxin type D gene and SCC<italic>mec</italic> IV. One CA-MRSA isolate was confirmed in a wound swab taken from a 47-year-old male, and the second was isolated from blood cultures of a 69-year-old female. No epidemiological connections between them were found.</p></sec><sec id="j_sjph-2016-0018_s_383_w2aab3b8c13b1b7b1aab1c15b4Aa"><h3>Conclusions</h3><p>In Slovenia CA-MRSA infections caused by ST80 are rare. In the future, it is necessary that a surveillance study of CA-MRSA at the national level continues and CA-MRSA be considered as a public health threat.</p></sec></abstract>

IntroductionAccording to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) circulate in Europe. In Europe, the European clone that belongs to sequence type ST80 is predominant.MethodsThe aim of the study was to investigate the phenotypic and genotypic characteristics and epidemiological data of CA-MRSA ST80 and its occurrence in Slovenia. We retrospectively analyzed those CA-MRSA isolates that were isolated during microbiological procedures in microbiological laboratories between 2006 and 2013. Only CA-MRSA isolates from the national collection of CA-MRSA strains that belonged to ST80 (European clone) were analyzed. We determined the Pantone-Valentine leukocidin (PVL), mec A genes, exfoliative toxin genes and type of staphylococcal cassette chromosome (SCCmec) by polymerase chain reaction (PCR). We determined also spa type and sequence type.ResultsST80 was confirmed in only 2 (0.5%) out of 385 CA-MRSA isolates, collected in a national collection of CAMRSA. Both isolates were positive for the PVL genes, mec A gene, exfoliative toxin type D gene and SCCmec IV. One CA-MRSA isolate was confirmed in a wound swab taken from a 47-year-old male, and the second was isolated from blood cultures of a 69-year-old female. No epidemiological connections between them were found.ConclusionsIn Slovenia CA-MRSA infections caused by ST80 are rare. In the future, it is necessary that a surveillance study of CA-MRSA at the national level continues and CA-MRSA be considered as a public health threat.

Infections Caused By Community-Associated Methicillin-Resistant <italic>Staphylococcus Aureus</italic> European Clone (ST80) In Slovenia Between 2006 And 2013

National Laboratory for Health, Environment and Food (NLZOH), Department of Medical Microbiology, Gosposvetska ulica 12, 4000 Kranj,

University Medical Centre Ljubljana, Department of Infectious Diseases, Japljeva 2, 1000 Ljubljana,

National Laboratory for Health, Environment and Food (NLZOH), Department of Medical Microbiology, Mej vrti 5, 8000 Novo mesto,

National Institute of Public Health (NIJZ), Regional Unit Novo mesto, Mej vrti 5, 8000 Novo mesto,

Institute of Microbiology and Parasitology, University of Ljubljana, Veterinary Faculty, Gerbičeva 60, 1000 Ljubljana,

Slovenian Journal of Public Health

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Infections Caused By Community-Associated Methicillin-Resistant  European Clone (ST80) In Slovenia Between 2006 And 2013

IntroductionAccording to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant Staphylococcus...

Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013

Article Category: Original scientific article

Online veröffentlicht: 11. Feb. 2016

Seitenbereich: 131 - 135

Eingereicht: 09. Sept. 2015

Akzeptiert: 09. Dez. 2015

DOI: https://doi.org/10.1515/sjph-2016-0018

Schlüsselwörter
CA-MRSA, ST80 (European clone), invasive infections, death, Slovenia

© National Institute of Public Health, Slovenia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013

Article Category: Original scientific article

Online veröffentlicht: 11. Feb. 2016

Seitenbereich: 131 - 135

Eingereicht: 09. Sept. 2015

Akzeptiert: 09. Dez. 2015

DOI: https://doi.org/10.1515/sjph-2016-0018

SchlüsselwörterCA-MRSA, ST80 (European clone), invasive infections, death, Slovenia

© National Institute of Public Health, Slovenia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
CA-MRSA, ST80 (European clone), invasive infections, death, Slovenia