<abstract xmlns="http://www.w3.org/1999/xhtml">Herein, an effort was made to investigate the antibacterial potential of agarose nanoparticles (ANPs) and poly(quaternary ammonium) modified ANPs (mANPs) against <italic>Escherichia coli</italic> (gram-negative bacterium) and <italic>Staphylococcus aureus</italic> (gram positive bacterium) in liquid systems as well as on agar plates. ANPs were synthesized by nanoprecipitation technology and characterized by XRD, TEM, TGA, DTA and DLS. The particle size estimated was 30 nm while atomic force microscopy was used to observe the interaction of ligand on ANPs. Antimicrobial characterization was monitored by colony forming units (CFU) as a function of ANPs concentration on agar plates. It was observed that ANPs showed 15 × 109/ml CFU after 24 hours of incubation at 20 mM ANPs concentration while the modified ANPs exhibited 21 × 109/ml CFU under similar incubation conditions. Moreover, zone of inhibition (ZOI) was 2.9 and 3.8 cm, respectively for <italic>E. coli</italic> by ANPs at 0.2 and 0.4 mM, respectively while it was 3.2 and 3.8 cm respectively by modified ANPs under similar conditions. Similarly, ZOI for <italic>S. aureus</italic> by ANPs at 0.2 and 0.4 mM was observed at 3.1 and 4.0 cm, respectively, while these values were 3.5 and 4.1 cm, respectively for modified ANPs under similar incubation conditions.</abstract>

Herein, an effort was made to investigate the antibacterial potential of agarose nanoparticles (ANPs) and poly(quaternary ammonium) modified ANPs (mANPs) against Escherichia coli (gram-negative bacterium) and Staphylococcus aureus (gram positive bacterium) in liquid systems as well as on agar plates. ANPs were synthesized by nanoprecipitation technology and characterized by XRD, TEM, TGA, DTA and DLS. The particle size estimated was 30 nm while atomic force microscopy was used to observe the interaction of ligand on ANPs. Antimicrobial characterization was monitored by colony forming units (CFU) as a function of ANPs concentration on agar plates. It was observed that ANPs showed 15 × 109/ml CFU after 24 hours of incubation at 20 mM ANPs concentration while the modified ANPs exhibited 21 × 109/ml CFU under similar incubation conditions. Moreover, zone of inhibition (ZOI) was 2.9 and 3.8 cm, respectively for E. coli by ANPs at 0.2 and 0.4 mM, respectively while it was 3.2 and 3.8 cm respectively by modified ANPs under similar conditions. Similarly, ZOI for S. aureus by ANPs at 0.2 and 0.4 mM was observed at 3.1 and 4.0 cm, respectively, while these values were 3.5 and 4.1 cm, respectively for modified ANPs under similar incubation conditions.

Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology

Ibn Sina National College for Medical Sciences, Department of Biochemistry, Jeddah-21418, Kingdom of Saudi Arabia

Ibn Sina National College of Medical Studies, Department of Pharmaceutical Sciences, Jeddah-21418, Kingdom of Saudi Arabia

King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Kingdom of Saudi Arabia

Polish Journal of Chemical Technology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Herein, an effort was made to investigate the antibacterial potential of agarose nanoparticles (ANPs) and poly(quaternary ammonium) modified ANPs (mANPs)...

Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology

Online veröffentlicht: 30. Juni 2016

Seitenbereich: 9 - 12

DOI: https://doi.org/10.1515/pjct-2016-0022

Schlüsselwörter
agarose nanoparticles, antibacterial properties, surface modification, nanotechnology

© 2016 Rukhsana Satar et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigating the antibacterial potential of agarose nanoparticles synthesized by nanoprecipitation technology

Online veröffentlicht: 30. Juni 2016

Seitenbereich: 9 - 12

DOI: https://doi.org/10.1515/pjct-2016-0022

Schlüsselwörteragarose nanoparticles, antibacterial properties, surface modification, nanotechnology

© 2016 Rukhsana Satar et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
agarose nanoparticles, antibacterial properties, surface modification, nanotechnology