The aim of the investigation was is to identify differences in an elemental composition (P, Fe, Mn, Zn and Cu) and their total flavonoids contents of medicinal herbs originating from 9 botanical species, harvested in Poland, Lithuania and Ukraine. Metallic elements were determined by FAAS technique in mg∙kg-1 of dry weight (d. wt) in the order: Fe > Mn > Zn > Cu in plant materials, and in infusions: Mn > Zn > Fe > Cu. The ratio of water-soluble form to total amount of a metal was as follows: 66.2% for Cu, 22.4% for Mn, 19.7% for Zn and 3.8% for Fe. The contents of P total, P inorganic and total flavonoids were determined by UV/Vis spectroscopic methods. By using of analysis of variance, correlation and cluster analyses it has been shown that a significant impact on the diversity of samples has the genetic factor - belonging to a particular botanical species of medicinal plant. The origin of investigated plants proved to be statistically significant only in the case of total Fe concentration, which was higher (α < 0.05) for the samples grown in Ukraine. A number of significant correlations was also obtained (r > 0.7) between: P total - P inorganic, Mn total - Mn extractable and Cu total - Cu extractable, also ( r > 0.5) between: total flavonoids - Cu extractable, P total - Cu total, P total - Cu extractable, P inorganic - Zn total. Moreover, comparison of the results of P, Fe, Mn, Zn and Cu determination in infusions of medicinal plants with the norms of RDA has shown that a significant amount (several percentage) of Mn and Cu can be supplemented to human organism with 2 cups of infusions prepared from Helichrysi inforescentia and Hyperici herba.