Uneingeschränkter Zugang

Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing

Muqiu Zhao
Muqiu Zhao
, 
Hui Wang
Hui Wang
, 
Shuai Wang
Shuai Wang
, 
Qiuying Han
Qiuying Han
 und 
Yunfeng Shi
Yunfeng Shi
   | 26. Nov. 2020
Oceanological and Hydrobiological Studies's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
Article Category: Research Article
Online veröffentlicht: 26. Nov. 2020
Seitenbereich: 408 - 420
Eingereicht: 10. Apr. 2020
Akzeptiert: 25. Mai 2020
DOI: https://doi.org/10.1515/ohs-2020-0036
© 2020 Faculty of Oceanography and Geography, University of Gdańsk, Poland
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Location of five sampling sites with four monospecific meadows and the bare tidal flat at the intertidal zone of Xincun Bay
Location of five sampling sites with four monospecific meadows and the bare tidal flat at the intertidal zone of Xincun Bay

Figure 2

Solution 31P-NMR spectra for NaOH-EDTA extracts of the sediments from different seagrass meadows
Solution 31P-NMR spectra for NaOH-EDTA extracts of the sediments from different seagrass meadows

Figure 3

Proportion of P forms in solution 31P-NMR spectra for NAOH-EDTA
Proportion of P forms in solution 31P-NMR spectra for NAOH-EDTA

Figure 4

NMDS plots of the sediment bacterial (A) and fungal OTUs (B) based on the unweighted UniFrac metric for all samples
NMDS plots of the sediment bacterial (A) and fungal OTUs (B) based on the unweighted UniFrac metric for all samples

Figure 5

Relative abundance of bacterial (A) and fungal (B) phyla
Relative abundance of bacterial (A) and fungal (B) phyla

Figure 6

Relative abundance of the top five genus-level P-cycling-related bacteria (A) and fungi (B) detected by 31P-NMR. Blue arrows represent different genera of microbes, while red arrows represent P forms. Correlation between P forms and RDA axes is shown by both the length and angle of the arrows.
Relative abundance of the top five genus-level P-cycling-related bacteria (A) and fungi (B) detected by 31P-NMR. Blue arrows represent different genera of microbes, while red arrows represent P forms. Correlation between P forms and RDA axes is shown by both the length and angle of the arrows.

Figure 7

Redundancy analysis (RDA) for the top five genus-level P-cycling-related microbes (A for bacteria and B for fungi) and sediment P forms detected by 31P-NMR. The blue arrows represent different genera of microbes, whereas variables for P forms are shown with red arrows. The correlation between P forms and RDA axes are shown by both the length and angle of the arrows.
Redundancy analysis (RDA) for the top five genus-level P-cycling-related microbes (A for bacteria and B for fungi) and sediment P forms detected by 31P-NMR. The blue arrows represent different genera of microbes, whereas variables for P forms are shown with red arrows. The correlation between P forms and RDA axes are shown by both the length and angle of the arrows.

Figure 8

Main findings of this research
Main findings of this research

The number of operational taxonomic units (OTUs) and Alpha diversity indices of bacterial and fungal communities. The values are means followed by a standard error. Different letters indicate statistically significant differences (p < 0.05) according to the Duncan test.

Samples OTUs Shannon Index Simpson Index Ace Index Chao Index
E. acoroides 1832 (124)a 5.72 (0.24)a 0.012 (0.004)a 2147 (104)a 2142 (99)a
T. hemprichii 2091 (6)b 5.88 (0.22)a 0.011 (0.005)a 2426 (53)b 2428 (59)b
C. rotundata 2081 (54)b 5.72 (0.39)a 0.019 (0.015)a 2424 (15)b 2414 (48)b
Bacteria H. ovalis 2105 (103)b 6.08 (0.11)a 0.007 (0.001)a 2392 (83)b 2408 (95)b
bare tidal flat 1937 (15)a 5.93 (0.07)a 0.009 (0.001)a 2313 (38)b 2316 (63)b
F test 7.427 1.233 1.058 9.295 7.555
p value 0.005 0.357 0.426 0.002 0.005
E. acoroides 299 (63)b 3.91 (0.45)b 0.064 (0.033)a 303 (65)b 303 (65)b
T. hemprichii 219 (40)ab 2.82(0.48)a 0.188 (0.093)b 226 (41)ab 231 (43)ab
C. rotundata 207 (58)a 4.12 (0.41)b 0.040 (0.023)a 211 (58)a 210 (57)a
Fungi H. ovalis 251 (22)ab 3.87 (0.19)b 0.071 (0.007)a 256 (21)ab 259 (26)ab
bare tidal flat 241 (27)ab 4.37 (0.03)b 0.029 (0.002)a 246 (28)ab 246 (29)ab
F test 1.907 8.451 5.796 1.810 1.694
p value 0.186 0.003 0.011 0.203 0.227
eISSN:
1897-3191
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
4 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Chemie, andere, Geowissenschaften, Biologie
Zeitschrift RSS Feed