Rare calciphilous diatoms from the genus Gomphonema (Bacillariophyta) in lotic waters of SE Poland
Article Category: Original research paper
Online veröffentlicht: 13. März 2018
Seitenbereich: 27 - 40
Eingereicht: 23. Juni 2017
Akzeptiert: 08. Sept. 2017
DOI: https://doi.org/10.1515/ohs-2018-0004
© 2018 Faculty of Oceanography and Geography, University of Gdańsk, Poland
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The genus
Many species of
The aim of this work was to present morphological descriptions and ecological characteristics of rare species from the
The research was conducted in the lotic waters of SE Poland. The area is drained by two large rivers – the San and the Wisłoka, the right-bank tributaries of the Vistula River that belongs to the Baltic Sea catchment area (Fig. 1). The San is the largest Carpathian river, with a length of 443.4 km. Its sources are in Ukraine. The upper basin section of this river is one of the most valuable natural areas in Poland and covers over 90 km of the river, from its source to the dam reservoir in the Solina village. For the first 50 kilometres, it is a foothill river, then having the features of a mountain river. The San River valley is characterized by shallow, steep banks and a broad riverbed consisting of typical Carpathian flysch strata (Kukuła 2002; Sieradzki 2002). One of the major tributaries of the upper San River is the Wołosaty stream, which flows through the area of Bieszczady National Park (BdNP).
Location of the study sites where species from the Figure 1
Study sites were designated in the San River (Fig. 1, S1) and in the Wołosaty stream with tributaries (Terebowiec and Rzeczyca) – Fig. 1, S2.
The sources of the Wisłoka River are in the Low Beskids area, at an altitude of 664 m a.s.l. The length of the river is 164 km and the basin area is 4110 km2. The upper and middle parts of the catchment area are mainly composed of Cretaceous and Tertiary flysch strata, the same as the San River (Buszko & Kiryk 1995; Boratyn & Brud 1996). Large water-level fluctuations are typical for mountain rivers. In rainless periods, the flows in streams are very low and in summer, the water temperature in shallow and wide channels is high (Soja 2009). The study sites were designated on the Wisłoka River and its tributaries (Wilsznia, Kłopotnica, Ryjak, Krempna, Rzeszówka, Świerzówka, Zimna Woda and Baranie), mainly in the area of Magura National Park (MNP) and the buffer zone (Fig. 1, S3).
The study area is not subject to strong human impact due to the existence of two national parks (BdNP and MNP). The area is sparsely populated with low tourist traffic and small, single farms outside the park.
Both rivers (the San and the Wisłoka) and their tributaries are characterized by rocky bottoms, with a predominance of medium stones. The study sites on the San and the Wisłoka were usually well sunlit, while the sites on the tributaries of these rivers were mostly shaded by trees and shrubs growing in the vicinity.
The material for analysis was collected from 8 sites in the upper reaches of the San River in 2010–2011, in the Wisłoka River (6 sampling sites) and its selected tributaries (streams: Ryjak, Wilsznia, Kłopotnica, Rzeszówka, Świerzówka, Krempna, Zimna Woda and Baranie – 17 sampling sites in total) in 2013–2014 and from 9 sites in the Wołosaty stream and its tributaries (Rzeczyca and Terebowiec) in 2013–2015. Samples were collected twice per year – in spring (April and May) and autumn (September and October) from all available habitats (stones, mud and aquatic plants). At the same time, temperature, pH, dissolved oxygen and electrolytic conductivity were measured in situ, and water for chemical analysis was also collected.
Chemical analyses were conducted using a Thermo Scientific DIONEX ICS-5000 + DC Chromatograph device at the Faculty Laboratory for Analysis of Environment Health and Materials of Agricultural Origin at the University of Rzeszów.
The laboratory analysis of diatoms was carried out according to methods presented in the study by Kawecka (1980) on river algae. To obtain pure diatom valves, a portion of each sample was macerated in cleaning chromic mixture (mixture of sulfuric acid and potassium dichromate) and washed in a centrifuge (at 2500 rpm). Permanent diatom slides were mounted in Pleurax resin (refractive index 1.75).
Diatoms were identified and counted under a Carl Zeiss Axio Imager A2 light microscope (LM) equipped with a 100× Plan Apochromatic objective with differential interference contrast (DIC) for oil immersion (NA 1.4). For scanning electron microscope (SEM) observations, samples were coated with 20 nm of gold using a Turbo-Pumped Sputter Coater Quorum Q 150OT ES and observed under a Hitachi SU 8010 microscope at the Podkarpacie Innovative-Research Center of Environment at the University of Rzeszów.
Diatoms were identified according to: Krammer and Lange-Bertalot (1986), Reichardt (1999), Hofmann et al. (2011) and Levkov et al. (2016). The abundance of species in assemblages was determined by counting specimens within randomly selected microscope fields of view up to a total of 400 valves. Species with a content above 5% in a given diatom assemblage were defined as the most abundant (dominant taxa).
The studied rivers and streams in SE Poland were characterized by mostly alkaline or close to neutral pH of water. Weak acidic pH (6.0–6.5) was recorded mainly during spring periods with elevated water levels due to snow melting. Water at the study sites had low or medium conductivity values, i.e. the lowest values were measured in the upper reaches of the studied watercourses, while the highest ones – during the low water level in autumn. The content of nutrients (mainly phosphates) was low or very low, often below the limit of detection. Increased values of nitrates were recorded only at some sites (usually in MNP) in the spring season. The investigated waters were also characterized by a relatively high content of calcium ions – usually in the range of 40 to more than 70 mg l-1. Values below 20 mg l-1 Ca2+ were recorded only in spring, during elevated water levels in the upper reaches of rivers and streams (Tables 1 & 2).
Physicochemical water parameters measured in the studied rivers and streams with values of medians (given in parentheses)
River/stream
Temperature (°C)
pH
Conductivity (μS cm–1)
O2 (mg l–1)
San
6.5–14.5 (7.5)
6.5–8.2 (7.8)
131–530 (141)
no data
Wołosaty
5.5–12.2 (8.3)
6.4–8.8 (8.3)
78–278 (149)
10.2–11.4 (10.6)
Terebowiec
6.1–9.3 (8.3)
6.4–8.4 (8.2)
86–284 (140)
10.7–11.1 (10.9)
Rzeczyca
7.4–10.0 (7.8)
6.4–8.4 (7.4)
97–246 (157)
10.2–10.6 (10.2)
Wisłoka
8.8–16.5 (9.3)
6.5–9.6 (7.8)
92–402 (233)
8.6–12.3 (10.0)
Ryjak
11.3–12.5 (12.0)
6.0–7.6 (7.2)
96–406 (224)
9.6– 11.2 (10.0)
Wilsznia
10.7–14.0 (12.1)
6.1– 7.5 (7.2)
104–420 (293)
9.6–10.2 (9.9)
Kłopotnica
11.1–16.0 (13.4)
6.3–7.5 (7.1)
84–325 (290)
9.6–10.0 (9.8)
Krempna
9.8–14.8 (12.7)
6.3–8.6 (7.7)
161–530 (356)
7.4–11.7 (9.8)
Rzeszówka
11.6–12.0 (11.8)
6.0–8.4 (6.9)
103–315 (212)
9.0–11.0 (10.1)
Świerzówka
10.8–12.5 (11.2)
6.3–8.6 (7.0)
101–313 (209)
9.0–11.5 (10.5)
Zimna Woda
10.1–13.0 (12.1)
6.4–8.5 (7.3)
146–418 (245)
9.4–9.9 (9.5)
Baranie
10.3–15.9 (10.9)
6.3–8.5 (7.7)
78–307 (245)
9.2–9.7 (9.3)
Concentration of ions measured in the studied rivers and streams with values of medians (given in parentheses)
River/stream
Cl– (mg l–1)
Ca2+(mg l–1)
San
0.8–3.4 (2.24)
11.4–24.9 (15.83)
1.0–2.9 (1.45)
<0.001
46.4–47.3 (47.1)
Wołosaty
0.4–5.4 (0.88)
11.1–26.8 (14.68)
1.7–3.2 (2.18)
<0.001–0.6
30.3–41.6 (36.75)
Terebowiec
0.4–5.6 (0.75)
12.5–27.2 (12.58)
2.0–2.9 (2.26)
<0.001
32.4–35.3 (33.31)
Rzeczyca
0.6–5.3 (0.98)
16.3–33.3 (16.60)
1.8–3.2 (2.3)
<0.001
38.8–39.7 (39.20)
Wisłoka
0.4–7.2 (5.7)
3.7–48.7 (29.7)
< 0.1–5.3 (0.5)
<0.001–4.2 (0.1)
23.3–62.5 (48.9)
Ryjak
0.1–7.0 (2.29)
2.5–30.8 (18.42)
< 0.1–5.1 (0.1)
0.03–0.04 (0.01)
26.0–62.6 (40.89)
Wilsznia
0.3–5.8 (1.41)
3.4–28.4 (18.09)
< 0.1–5.8 (0.12)
<0.001–0.2 (<0.01)
15.8–58.1 (38.74)
Kłopotnica
0.4–8.5 (1.37)
4.3–51.1 (17.74)
0.4–7.2 (5.15)
0.01–0.02 (0.01)
13.4–39.8 (34.6)
Krempna
0.7–1.6 (1.14)
6.0–46.3 (16.15)
< 0.1–5.4 (1.80)
<0.001–0.07 (0.01)
33.7–76.4 (54.2)
Rzeszówka
0.8–1.2 (1.0)
10.1–27.3 (19.30)
< 0.1–4.2 (0.85)
0.02–0.09 (0.03)
18.9–43.9 (33.5)
Świerzówka
0.5–1.2 (0.7)
10.1–27.6 (19.5)
< 0.1–5.0 (0.95)
0.008–0.03 (0.01)
18.8–49.1 (42.3)
Zimna Woda
0.6–5.6 (3.35)
8.8–38.4 (24.73)
1.2–6.7 (1.4)
0.01–0.05 (0.02)
38.6–42.3 (40.07)
Baranie
0.7–4.9 (2.84)
7.1–24.0 (21.54)
0.6–10 (1.7)
<0.001–0.02 (0.01)
12.4–41.1 (38.5)
The
Examples of morphological variation in LM: Gomphonema cuneolus (A–Y); Figure 2
Examples of morphological variation in LM: Figure 3
Figure 4
length 12.7–27.5 μm, width 2.8–4.4 μm, number of striae 10–12 in 10 μm. Individual cells were slightly longer and narrower in relation to the dimensions reported by Reichardt (1997). Longer cells (up to 28.7 μm) were also reported by Ector et al. (2015) from France.
stones and mosses.
The species usually occurred individually at all sites in the Wisłoka River (Fig. 1, S3: 1, 2 & 4–6) and its tributaries: Kłopotnica (Fig. 1, S3: 21 & 22), Świerzówka (Fig. 1, S3: 19), Rzeszówka (Fig. 1, S3: 20), Krempna (Fig. 1, S3: 10 & 11), Zimna Woda (Fig. 1, S3: 12 & 13) and Baranie (Fig. 1, S3: 14 & 15). In the Świerzówka in August 2013, the number of valves increased to 3.2%. Single cells were also observed in the Wołosaty (Fig. 1, S2: 1 & 3) stream and the Terebowiec tributary (Fig. 1, S2: 8 & 9).
species new to Poland.
Reported by Cantonati et al. (2016) from springs in Italy and by Ector et al. (2015) from France. Also included on the species list from inland waters in North America (Bahls 2009).
There is no detailed information on the ecology of the species. According to Reichardt (1997), it seems to prefer calcium-rich waters.
length 22.5–41.0 μm, width 6.6–8.4 μm, number of striae 8–11 in 10 μm.
It is usually found among mosses, and less often on stones and mud.
The species was very rare and occurred in the form of single cells in the Wołosaty stream (BdNP), (Fig. 1, S2: 3) and in the Wisłoka (Fig. 1, S3: 3 & 4) and the Kłopotnica (Fig. 1, S3: 22) (MNP). Individual specimens were also found in the San River (Fig. 1, S1: 2).
At sites in the Wisłoka and Kłopotnica rivers,
Species new to Poland, known only from flowing waters in SE Poland (Noga et al. 2016a,b).
Species reported from Europe (Reichardt 1999; Levkov et al. 2016) and North America (Bahls 2009).
The species was described by Reichardt (1999) from calcium-rich puddles, where it co-occurred with
length 16.7–22.0 μm, width 4.0–5.8 μm, number of striae 13–16 in 10 μm.
Individual cells were narrower and had less dense striae than those described by Reichardt (1999) and Levkov et al. (2016).
mosses and stones.
Individual specimens found only in the upper reaches of the Wisłoka River (Fig. 1, S3: 1) near
Reported from springs in central Poland (Żelazna-Wieczorek 2011).
Reported from other European countries more often than from Poland, e.g. SW Serbia (Vidaković et al. 2016), southern and western Bulgaria (Ivanov et al. 2006) and Macedonia (Levkov et al. 2016). The species is also known from inland waters in North America (Kociolek 2005; Bahls 2009).
The species was described for the first time from southern Germany, where it was observed in standing waters (fish ponds or backwaters), medium rich in electrolytes, and meso- to eutrophic. The occurrence of the species is not precisely determined (Reichardt 1999; Hofmann et al. 2011). The species was reported from two sites in Macedonia (Bistra mountain), from small oligotrophic ponds located on calcareous bedrock (Levkov et al. 2016).
Figure 5
length 17.2–59.7 μm, width 4.6–7.0 μm, number of striae 9–12 (in central area), 14–18 (closer to the end) in 10 μm. Many cells were narrower than those described by Reichardt and Lange-Bertalot (1991), while observations by Levkov et al. (2016) conducted in Macedonia were consistent with our observations.
Observed mainly on stones, less often on mud and among mosses.
Found in the Wisłoka (Fig. 1, S3: 2–6), the Wilsznia (Fig. 1, S3: 16–18), the Ryjak (Fig. 1, S3: 7) and the Kłopotnica (Fig. 1, S3: 21 & 22) and in the streams: Świerzówka (Fig. 1, S3: 19), Rzeszóowka (Fig. 1, S3: 20), Zimna Woda (Fig. 1, S3: 12 & 13) and Baranie (Fig. 1, S3: 14 & 15), only in the form of single cells. The species was more abundant in the Wisłoka River in October 2013 (accounted for about 1% of the assemblage). In the BdNP area, it was found in the streams Wołosaty (Fig. 1, S2: 1, 3 & 4) and Terebowiec (Fig. 1, S2: 8 & 9), always individually.
In the Wisłoka River, where
Reported from Lake Rosnowskie Duże in the Wielkopolska National Park (Celewicz-Gołdyn 2006) and from springs in central Poland (Żelazna-Wieczorek 2011).
species known i.a. from Austria (Gesierich & Koffler 2010), Germany (Reichardt & Lange-Bertalot 1991; Lange-Bertalot 1996; Täuscher 2014, 2016), Belgium (Denys 2000), Macedonia (Levkov & Williams 2012), Great Britain (Whitton et al. 2003) and North America (Bahls 2009).
Studies carried out in lotic waters in SE Poland show that diatoms occur in many places, both in larger rivers (San, Wisłoka) and small tributaries. At each study site, they occurred as single cells, with up to 1% contribution to the assemblage. Most specimens were observed in the San and the Wisłoka, usually in autumn. Water was alkaline (pH 7.3–8.0), with medium or high conductivity, low content of nutrients and high content of calcium (40 to more than 60 mg l-1).
This information is confirmed by literature data where
Scanning microscope micrographs of Figure 6
length 27.0–44.5 μm, width 7.0–9.9 μm, number of striae 10–11 in 10 μm. Individual cells were slightly narrower and had lower striation density compared to those described by Reichardt (1999).
stones, mud and mosses.
In the Low Beskids (MNP), where the species occurred only as single cells, in the Wisłoka (Fig. 1, S3: 2–6), the Wilsznia (Fig. 1, S3: 16 & 17) the Kłopotnica (Fig. 1, S3: 23), the Krempna (Fig. 1, S3: 11), the Świerzówka (Fig. 1, S3: 19) and the Baranie (Fig. 1, S3: 14). In the Bieszczady National Park, where the species occurred individually, in the Wołosaty (Fig. 1, S2: 1 & 3–6) and the Rzeczyca (Fig. 1, S2: 7), and in the San River, where it occurred in the form of single cells, but almost at all sites (Fig. 1, S1: 1, 3 & 5–7).
In the Wisłoka River and its tributaries, the most abundant were:
species new to Poland.
Reported from Central Europe and the Balkans (Reichardt 1999, 2015; Hofmann et al. 2011).
Rarely reported, mainly from calcium-rich waters in alpine areas, especially in lakes in the limestone Alps of Central Europe and from the Balkans. The species is widespread in calcareous waters of Central Europe (Reichardt 1999; Hofmann et al. 2011), but according to Reichardt (2015), some specimens may be confused with
length 10.5–36.1 μm, width 4.6–6.7 μm, number of striae 10–15 in 10 μm.
Many cells were much longer and wider than those described in the literature. Large cells had often fewer striae per 10 μm (10–11) (Hofmann et al. 2011; Bąk et al. 2012; Levkov et al. 2016).
Most abundant on stones, single specimens on mud and mosses.
In Magura National Park, the species was described at most study sites, both in the Wisłoka River (Fig. 1, S3: 1, 2 & 4–6) and its tributaries: Wilsznia (Fig. 1, S3: 116 & 17), Kłopotnica (Fig. 1, S3: 21–23), Krempna (Fig. 1, S3: 10 & 11), Rzeszówka (Fig. 1, S3: 20), Zimna Woda (Fig. 1, S3: 12 & 13) and Baranie (Fig. 1, S3: 14 & 15). It usually occurred alone. It reached larger numbers (2–3%) only in spring in the source reaches of the Wisłoka (Fig. 1, S3: 1–3), the Krempna (Fig. 1, S3: 10) and the Wilsznia (Fig. 1, S3: 16), and about 8% in the sources of the Baranie stream (Fig. 1, S3: 14). In the Bieszczady National Park, the taxon was observed individually at all sites (Fig. 1, S2: 1–9). Larger populations were observed only in the spring season in the Terebowiec stream (2–3%), (Fig. 1, S2: 8 & 9).
In the Wisłoka River and its tributaries (MNP), where
Reported from over a dozen sites in Poland as
For the past several years, the species has been described from various places around the world, e.g. oligotrophic mountain rivers in Europe (often from upper reaches) and Canadian rivers, the Himalayas and New Zealand (Cantonati et al. 2001; Werum & Lange-Bertalot 2004; Goma` et al. 2005; Dohet et al. 2008; Gesierich & Rott 2012; Harper et al. 2012; Levkov et al. 2016).
Scattered, occurring in waters poor in electrolytes and calcium, circumneutral to slightly acidic. Further research is needed to better understand the autecology of this species (Hofmann et al. 2011; Bąk et al. 2012). Levkov et al. (2016) often observed
All the described species from the genus
Based on the literature data, it appears that
The valuable places in SE Poland, mentioned in the presented paper, provide many interesting facts about habitats of calciphilous species, useful for a better understanding of their ecology.