This article was focused on the evaluation of neutral salt spray test effect (according to ISO 9124), procedure E4, on mechanical properties of load-bearing adhesive joints. The study also comprises the influence of substrate material (mild steel, aluminum alloys) and substrate surface roughness (verified by optical confocal microscopy). The experimental program contained acrylate-based adhesive and hybrid silicone polyurethane based adhesive which were applied in double lap joints loaded by shear.