<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.</p></abstract>

Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.

Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Introduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to...

Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

Online veröffentlicht: 19. Sept. 2017

Seitenbereich: 321 - 327

Eingereicht: 06. Juni 2017

Akzeptiert: 31. Aug. 2017

DOI: https://doi.org/10.1515/jvetres-2017-0032

Schlüsselwörter
food of animal origin, chloramphenicol, residues, analytical method

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

Online veröffentlicht: 19. Sept. 2017

Seitenbereich: 321 - 327

Eingereicht: 06. Juni 2017

Akzeptiert: 31. Aug. 2017

DOI: https://doi.org/10.1515/jvetres-2017-0032

Schlüsselwörterfood of animal origin, chloramphenicol, residues, analytical method

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
food of animal origin, chloramphenicol, residues, analytical method