<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during <italic>in vitro</italic> culture.<bold>Material and Methods:</bold> The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, <italic>i.e</italic>. aneuploidy and polyploidy, and structural aberrations, <italic>i.e.</italic> breaks in chromosomes and chromatid, were taken into account during the study.<bold>Results:</bold> The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells.<bold>Conclusion:</bold> Mesenchymal stem cells from foal umbilical cords during <italic>in vitro</italic> cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.</abstract>

Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture.Material and Methods: The mesenchymal stem cells were obtained from equine bone marrow of three horses and from foal umbilical cords of six foals. The cells were cultured in CO2 incubators by standard procedure. Quantitative abnormalities of chromosomes, i.e. aneuploidy and polyploidy, and structural aberrations, i.e. breaks in chromosomes and chromatid, were taken into account during the study.Results: The results of cytogenetic analysis of equine bone marrow mesenchymal stem cells at the third and fourth passages indicated that the level of karyotype variability of these cells corresponded to the spontaneous level of karyotype variability typical of the peripheral blood lymphocytes of this species. Equine bone marrow contained several clones of stem cells that differed in the expression of specific nuclear markers characteristic of proliferating cells.Conclusion: Mesenchymal stem cells from foal umbilical cords during in vitro cultivation are characterised by quantitative abnormalities of the chromosomal apparatus.

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during <italic>in vitro</italic> culture

Department of Physiology, Pathophysiology and Immunology of Animals, National University of Life and Environmental Sciences of Ukraine, 03041 Kyiv,

Department of Experimental Cell Systems, R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, 03022 Kyiv,

Laboratory of Genetics, Institute of Animal Breeding and Genetics, National Academy of Agrarian Sciences of Ukraine, 08321 Kyiv,

Equestrian Federation of Ukraine, 01054 Kyiv,

Department of Biological Basis of Animal Production, Faculty of Biology and Animal Breeding, University of Life Sciences in Lublin, 20-950 Lublin,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during  culture

Introduction: The objective of the study was immunophenotypic and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical...

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

Online veröffentlicht: 23. Sept. 2016

Seitenbereich: 339 - 347

Eingereicht: 18. Jan. 2016

Akzeptiert: 21. Juli 2016

DOI: https://doi.org/10.1515/jvetres-2016-0051

Schlüsselwörter
horses, bone marrow, umbilical cord, mesenchymal cells, cytogenetic analysis

© 2016 Anatoliy Mazurkevych et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Immunophenotypic characterisation and cytogenetic analysis of mesenchymal stem cells from equine bone marrow and foal umbilical cords during in vitro culture

Online veröffentlicht: 23. Sept. 2016

Seitenbereich: 339 - 347

Eingereicht: 18. Jan. 2016

Akzeptiert: 21. Juli 2016

DOI: https://doi.org/10.1515/jvetres-2016-0051

Schlüsselwörterhorses, bone marrow, umbilical cord, mesenchymal cells, cytogenetic analysis

© 2016 Anatoliy Mazurkevych et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
horses, bone marrow, umbilical cord, mesenchymal cells, cytogenetic analysis