<abstract xmlns="http://www.w3.org/1999/xhtml">The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and ‘Otong’ (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents’ alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents.</abstract>

The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and ‘Otong’ (male parent), was conducted in order to determine the genetic characteristics of hybrids and parents, as it would be followed/evidenced by the variability of traits produced from the cross breeding. The RAPD analyses of 14 primers resulted in 114 scoring bands, 112 (98.2%) of them were polymorphic, with 4 to 11 bands amplified per primer. The electrophoresis gel of the PCR results revealed that some hybrids produced different band patterns compared to the parents; this indicated the crossing between parents’ alleles and trait combinations from both the parents. The Dice-Sorensen similarity coefficient demonstrated that most of the hybrids had distant genetic similarities with both parents, which were ranged from 0.141 [71B(4) and 72B(15)] to 0.776 [71B(15) and 48B(1)]. The UPGMA method was used to construct the dendrogram, which grouped the hybrids in five clusters with distinct genetic relationships and was confirmed with the PCA analysis. This result implied that above crossing produced hybrids having characters different from the parents.

Genomic Profiling of F1 Hybrids of Durian (<italic>D<sc>urio zibethinus</sc></italic>) Revealed by RAPD-PCR

Indonesian Tropical Fruit Research Institute, Jalan Raya Solok-Aripan Km. 8 Solok 27301, Sumatra Barat,

Journal of Horticultural Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genomic Profiling of F Hybrids of Durian () Revealed by RAPD-PCR

The molecular analysis of 32 durian F1 hybrids, resulted from crossing of the Arp 8990 (female parent) and ‘Otong’ (male parent), was conducted in order to...

Genomic Profiling of F₁ Hybrids of Durian (Durio zibethinus) Revealed by RAPD-PCR

Online veröffentlicht: 30. Dez. 2016

Seitenbereich: 69 - 76

Eingereicht: 01. März 2015

Akzeptiert: 01. Nov. 2016

DOI: https://doi.org/10.1515/johr-2016-0022

Schlüsselwörter
cross breeding, hybrids, RAPD

© 2016 Riry Prihatini et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genomic Profiling of F1 Hybrids of Durian (Durio zibethinus) Revealed by RAPD-PCR

Online veröffentlicht: 30. Dez. 2016

Seitenbereich: 69 - 76

Eingereicht: 01. März 2015

Akzeptiert: 01. Nov. 2016

DOI: https://doi.org/10.1515/johr-2016-0022

Schlüsselwörtercross breeding, hybrids, RAPD

© 2016 Riry Prihatini et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genomic Profiling of F₁ Hybrids of Durian (Durio zibethinus) Revealed by RAPD-PCR

Schlüsselwörter
cross breeding, hybrids, RAPD