Genotoxicity and antigenotoxicity study of aqueous and hydro-methanol extracts of Spondias mombin L., Nymphaea lotus L. and Luffa cylindrical L. using animal bioassays

Abozed SS, El-kalyoubi M, Abdelrashid A, Salama MF. (2014). Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Annals of Agric Sci59(1): 63–67.Search in Google Scholar

Accioly MP, Bevilaqua CML, Fernana CM, Rondon FCM, de Morais SM, Machado LKA, Almeida CA, de Andrade Jr HF, Cardoso RPA. (2012). Leishmanicidal activity in vitro of Musa paradisiaca L. and Spondia smombin L. fractions. Vet Parasitol187: 79–84.Search in Google Scholar

Aduloju RK, Otubanjo OA, Odeigah PGC. (2008). An In Vivo Assay of the Mutagenic Potential of Praziquantel (PZQ) Using Sperm Head Abnormality Test. J Hum Ecol23(1): 59–63.Search in Google Scholar

Afolayan AJ, Sharaibi OJ, Kazeem MI. (2013). Phytochemical analysis and in vitro antioxidant activity of Nymphaea lotus L. Int J Pharmacol9(5): 297–304.Search in Google Scholar

Ahmad B, Khan AA. (2013). Antibacterial, antifungal and phytotoxic activities of Luffa cylindrica and Momordica charantia. J Med Plant Res7(22): 1593–1599.Search in Google Scholar

Aitken RJ, de Iuliis GN, McLachlan RI. (2009). Biological and clinical significance of DNA damage in the male germ line. Intern J Androl32: 46–56.Search in Google Scholar

Akinjogunla OJ, Yah CS, Eghafona NO, Ogbemudia FO. (2010). Antibacterial activity of leave extracts of Nymphaea lotus (Nymphaeaceae) on Methicillin resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Staphylococcus aureus (VRSA) isolated from clinical samples. Annals Biol Res1(2): 174–184.Search in Google Scholar

Akinmoladun AC, Obuotor EM, Barthwal MK, Dikshit M, Farombi EO. (2010). Ramipril-like activity of Spondias mombin Linn against no-flow ischemia and isoproterenol-induced cardiotoxicity in rat heart. Cardiovas Tox10(4): 295–305.Search in Google Scholar

Akouedegni CG, Koudande OD, Ahoussi E, Hounzangbe-Adote MS. (2013). Effects of the fresh leaves of Spondias mombin L. on milk production of West African Dwarf (WAD) ewes and their lamb’s growth performance. J Ani Sci Adv3(2): 74–82.Search in Google Scholar

Alabi OA, Bakare AA. (2011). Genotoxicity and mutagenicity of electronic waste leachates using animal bioassays. Tox Env Chem93(5): 1073–1088.Search in Google Scholar

Alvarez JG, Touchstone JC, Blascol JC, Storey BT. (1987). Spontaneous lipid peroxidation and hydrogen peroxide and superoxide in human spermatozoa: superoxide dismutase as major enzyme protectant against oxygen toxicity. J Androl18: 338–348.Search in Google Scholar

Alves ABCR, dos Santos RS, Calil SD, Niero R, Lopes JD, Perazzo FF, Rosa PCB, Andrade SF, Cechinel-Filho V, Maistro EL. (2014). Genotoxic assessment of Rubus imperialis (Rosaceae) extract in vivo and its potential chemoprevention against cyclophosphamide-induced DNA damage. J Ethnopharmacol153: 694–700.Search in Google Scholar

Asuquo OR, Udonwa UN, Eluwa MA, Ekanem TB. (2013a). Effects of Spondias Mombin leaf extract on the cytoarchitecture of the cerebal cortex and on learning and memory in Wistar Rats. Int J Sci Res2(9): 5–8.Search in Google Scholar

Asuquo RO, Ekanem BT, Udoh BP, Mesembe EO, Ebong EP. (2013b). Haematinic potential of Spondias Mombin leaf extract in Wistar Rats. Adv Biores4(2): 53–56.10.1016/S2305-0500(13)60141-4Search in Google Scholar

Asuquo OR, Oko OOK, Brownson ES, Umoetuk GB, Utin IS. (2013c). Effects of ethanolic leaf extract of Spondias mombin on the pituitary gonadal axis of female Wistar rats. Asian Pacific J Reprod2(3): 169–173.10.1016/S2305-0500(13)60141-4Search in Google Scholar

Ayoka AO, Akomolafe RO, Iwalewa EO, Ukponmwan OE. (2005). Studies on the anxiolytic effects of Spondias mombin L. (Anacardicaea) extracts. Afri J Trad Compl Alt Med2(2): 153–165.Search in Google Scholar

Ayoka AO, Akomolafe RO, Iwalewa EO, Akanmu MA, Ukponmwan OE. (2006). Sedative, antiepileptic and antipsychotic effects of Spondias mombin L. (Anacardiacaea) in mice and rats. J Ethnopharmacol103: 166–175.Search in Google Scholar

Bakare AA, Mosuro AA, Osibanjo O. (2005). An in vivo evaluation of induction of abnormal sperm morphology in mice by landfill leachates. Mutat Res582(1–2): 28–34.Search in Google Scholar

Bakkali F, Averbeck S, Averbeck D, Zhiri A, Idaomar M. (2005). Cytotoxicity and gene induction by some essential oils in the yeast Saccharomyces cerevisiae. Mutat Res585: 1–13.Search in Google Scholar

Balakrishnan N, Huria T. (2011). Protective effect of Luffa cylindrica L. fruit in Paracetamol induced hepatotoxicity in rats. Int J Pharm Biol Arch2(6): 1761–1764.Search in Google Scholar

Balakrishnan N, Sharma A. (2013). Preliminary phytochemical and pharmacological activities of Luffa cylindrica L. fruit. Asian J Pharm Clin Res6(2): 113–116.Search in Google Scholar

Briskin DP. (2000). Medicinal plants and phytomedicines linking plant biochemistry and physiology to human health. Plant Physiol124: 507–514.Search in Google Scholar

Cassidy SL, Dix KM, Jenkins T. (1983). Evaluation of a testicular sperm head counting technique using rats exposed to dimethoxyethyl phthalate (DMEP), glycerol alpha-monochlorohydrin (GMCH), epichlorohydrin (ECH), formaldehyde (FA), or methyl methanesulphonate (MMS). Arch Tox53(1): 71–78.Search in Google Scholar

Chaurasia S, Sharma V, Iqbal dar A, Arya N, Saxena RC, Chaurasia ID, Shrivastava R. (2011). In vivo antidiabetic activity of alcoholic and aqueous extract of Nymphaea lotus in rat model. Inventi Rapid: EthnopharmacolSearch in Google Scholar

Coates NJ, Gilpin ML, Guynn MN, Lewis DE, Milner PH, Spear SR, Tyler JW. (1994). A novel beta-lactamase inhibitor isolated from Spondias mombin. J Nat Prod57: 654–657.Search in Google Scholar

Craig GM, Newman DJ, Snader KM. (1997). Natural products in drug discovery and development. J Nat Prod60: 52–60.Search in Google Scholar

Cuyacot AR, Mahilum JJM, Madamba MSB. (2014). Cytotoxicity potentials of some medicinal plants in Mindanao, Philippines. Asian J Plant Sci Res 4(1): 81–89.Search in Google Scholar

Du Q, Xu Y, Li L, Zhao Y, Jerz G, Winterhalter P. (2006). Antioxidant constituents in the fruits of Luffa cylindrica (L.) Roem. J Agric Food Chem54(12): 4186–4190.Search in Google Scholar

Ferguson LR. (2001). Role of plant polyphenols in genomic stability. Mutat Res475: 89–111.Search in Google Scholar

Ferreira-Machado SC, Gagliardi RF, Nunes APM, Rodrigues MP, Dantas FJS, De Mattos JCP, Peregrin, CAF, Moura EG, Caldeira-de-Araujo A. (2014). Antidiabetic and genotoxic effects on Wistar rats treated with aqueous extract from Chrysobalanus icaco L. J Med Plants Res8(1): 52–57.Search in Google Scholar

Fred-Jaiyesimi A, Kio A, Richard W. (2009). α-Amylase inhibitory effect of 3β-olean-12-en-3-yl (9Z)-hexadec-9-enoate isolated from Spondias mombin leaf. Food Chem116: 285–288.Search in Google Scholar

Hamenoo NA. (2010). Hepatoprotective and toxicological assessment of spondias mombin L. (Anacardiaceae) in rodents. M. Phil thesis. Department of Pharmacology, Kwame Nkrumah University of Science and Technology. 83pp.Search in Google Scholar

Hazra M, KunduSen S, Bhattacharya S, Haldar PK, Gupta M, Mazumder UK. (2011). Evaluation of hypoglycemic and antihyperglycemic effects of Luffa cylindrica fruit extract in rats. J Adv Pharm Edu Res2: 138–146.Search in Google Scholar

Hugenholtz AP, Bruce WR. (1983). Radiation induction of mutations affecting sperm morphology in mice. Mutat Res107: 177–185.Search in Google Scholar

Hwang Y, Park H, Ma JY. (2013). In vitro and in vivo safety evaluation of Acer tegmentosum. J Ethnopharmacol148: 99–105.Search in Google Scholar

Idu M, Obaruyi GO, Erhabor JO. (2008). Ethnobotanical uses of plants among the Binis in the treatment of ophthalmic and ENT (EAR, Nose and Throat) ailments. Ethnobot Leaflets13: 480–496.Search in Google Scholar

Igwe CU, Ojiako AO, Nwaogu LA, Onyeze GOC. (2008). Lipid lowering effect of aqueous leaf extract of Spondias mombin Linn. The Internet J Pharmacol6(1): 1–9.Search in Google Scholar

Igwe CU, Ojiako OA, Nwaogu LA, Iwueke AV. (2012). Evaluation of the antioxidant activity of African plants: Activity of the aqueous leaf extract of Spondias mombin linn. J Res Pharmacol1: 1–9.Search in Google Scholar

Kamatenesi-Mugisha M, Makawiti DW, Oryem-Origa H, Olwa-Odyek, Nganga J. (2007). The oxytocic properties of Luffa cylindrica (L.) M. Roem. and Bidens pilosa L., traditionally used medicinal plants from western Uganda. Afr J Eco45: 88–93.Search in Google Scholar

Kanwal W, Syed AW, Salman A, Mohtashee HM. (2013). Anti-emetic and anti-inflammatory activity of fruit peel of Luffa cylindrica (L.) Roem. Asian J Nat Appl Sci2(2): 175–180.Search in Google Scholar

Khan KW, Ahmed SW, Ahmed S, Hasan MM. (2013). Antiemetic and anti-inflammatory activity of leaves and flower extracts of Luffa cylindrica (L.) Roem. The J Ethnobiol Tradl Med Photon118: 258–263.Search in Google Scholar

Ko RJ. 2004. A U.S. perspective on the adverse reactions from traditional Chinese medicines. J Chin Med Ass67: 109–116.Search in Google Scholar

Kothari S, Thompson A, Agarwal A, Plessis SS. (2010). Free radicals: their beneficial and detrimental effects on sperm function. Ind J Exp Biol48: 425–435.Search in Google Scholar

Krishna G, Hayashi M. (2000). In vivo rodent micronucleus assay: Protocol, conduct and data interpretation. Mutat Res455: 155–66S.Search in Google Scholar

Lather A, Valecha R, Sharma K, Garg M. (2011). Worldwide potential of plants causing teratogenicity: An overview. Spatula DD1(2): 101–106.Search in Google Scholar

Leffa DD, da Rosa R, Munhoz BP, Mello ADM, Mandelli FD, Amaral PD, Rossatto AE, de Andrade VM. (2012). Genotoxic and antigenotoxic properties of Calendula officinalis extracts in mice treated with methylmethane sulfonate. Adv Life Sci2(2): 21–28.Search in Google Scholar

Loomis TA, Hayes AW. (1996). Loomis’s Essentials of Toxicology, 4th ed. Academic Press, California, pp. 208–245.Search in Google Scholar

Mary RR, Begum SMI. (2014). Antimicrobial activity of different solvent extracts of Tridax procumbens (Asteraceae) from leaf and stem against Human pathogens. Int J Sci Res3(8): 487–489.Search in Google Scholar

Mao ZS, Xu ZC, Song XF, Ma QX. (2004). Effects of Luffa-extract on the macrophage function in mice. J Xinxiang Med Col21(2): 80–82.Search in Google Scholar

Muthumani P, Meera R, Mary S, Jeenamathew, Devi P, Kameswari B, Priya BE. (2010). Phytochemical screening and anti inflammatory, bronchodilator and antimicrobial activities of the seeds of Luffa cylindrica. Res J Pharm Biol Chem Sci1(4): 11–22.Search in Google Scholar

Nikolettos N, Kiipker W, Demirel C, Schopper B, Blasig C, Sturm R, Felberbaum R, Bauer O, Diedrich K, Al-Hasani S. (1999). Fertilization potential of spermatozoa with abnormal morphology. Hum Reprod14(Suppl. 1): 47–70.Search in Google Scholar

Nworu CS, Akah PA, Okoli CO, Okoye TC. (2007). Oxytocic activity of leaf extract of Spondias mombin (Anacardiaceae). Pharm Biol45(5): 366–371.Search in Google Scholar

Nworu CS, Akah PA, Okoye FBC, Toukam DK, Udeh J, Esimone CO. (2011). The leaf extract of Spondias mombin L. displays an anti-inflammatory effect and suppresses inducible formation of tumor necrosis factor-α and nitric oxide (NO). J Immunotox8(1): 10–16.Search in Google Scholar

Odeigah PC. (1997). Sperm head abnormalities and dominant lethal effects of formaldehyde in albino rats. Mutat Res389: 141–148.Search in Google Scholar

Odunola OA, Akinwumi KA, Ibegbu DM. (2011). The influence of garlic and Spondias mombin on Sodium arsenite induced clastogenicity and hepatotoxicity in rats. The Pacific J Sci Tech12(2): 401–409.Search in Google Scholar

Ola-Davies O, Ajani OS, Oyeyemi MO. (2014). Spermatozoa morphology and characteristics of Spondias mombin L. (Anacardiaceae) protected male Wistar rats exposed to sodium arsenite. J Vet Med Ani Health6(2): 63–66.Search in Google Scholar

Oyedare BM, Bakare AA, Akinboro A. (2009). Genotoxicity assessment of water extracts of Ocimum gratissimum, Morinda lucida and Citrus medica using the Allium cepa assay. BLACPMA8(2): 97–103.Search in Google Scholar

Oyeyemi IT, Bakare AA. (2013). Genotoxic and anti-genotoxic effect of aqueous extracts of Spondias mombin L., Nymphaea Lotus L. and Luffa cylindrica L. on Allium cepa root cells. Carylogia66(4): 360–367.Search in Google Scholar

Pakoussi T, Mouzou A, Metowogo K, Agbonon A, Eklu-Gadegbeku K, Aklikokou AK, Gbeassor M. (2013). Effects of Spondias mombin Linn (Anacardiaceae) on rat parturition. Int J Biol Chem Sci7(2): 441–446.Search in Google Scholar

Pal RJ, Manoj J. (2011). Hepatoprotective activity of alcoholic and aqueous extracts of fruits of Luffa cylindrica Linn in rats. Annals of Biol Res2(1): 132–141.Search in Google Scholar

Partap S, Kumar A, Sharma NK, Jha KK. (2012a). Luffa Cylindrica: An important medicinal plant. J Nat Prod Plant Resour2(1): 127–134.Search in Google Scholar

Partap S, Kumar S, Kumar A, Sharma NK, Jha KK. (2012b). In vitro anthelmintic activity of Luffa cylindrica leaves in Indian adult earthworm. J Pharmacognosy Phytochem1(2): 30–34.Search in Google Scholar

Pauly G, Fleury M. (2002). Cosmetic containing plant extracts. Official Gazette of U.S. Patents and Trademark Office. Patents, 1259(3).Search in Google Scholar

Pillay P, Phulukdaree A, Chuturgoon AA, Toit KD, Bodenstein J. (2013). The cytotoxic effects of Scilla nervosa (Burch.) Jessop (Hyacinthaceae) aqueous extract on cultured HepG2 cells. J Ethnopharmacol145: 200–204.Search in Google Scholar

Prakash YG, Ilango K, Kumar S, Elumalai A. (2010). In vitro antioxidant activity of Luffa cylindrica seed oil. J Global Pharma Tech2(3): 93–97.Search in Google Scholar

Raji Y, Gbadegesin MA, Osonuga OA, Adisa RA, Akinsomisoye OS, Awobajo FO, Kunle-Alabi OT, Esegbue Peters PRC, Osonuga IO, Lamidi AF. (2006). Reproductive, Haematogic and Biochemical profile of male rats treated with aqueous extract of S. mombin bark. Int J Pharmacol2(1): 126–130.Search in Google Scholar

Rodeiro I, Cancino L, Gonzalez JE, Morffi J, Garrido G, Gonzalez RM, Nunez A, Delgado R. (2006). Evaluation of the genotoxic potential of Mangifera indica L. extract (Vimang), a new natural product with antioxidant activity. Food Chem Tox44: 1707–1713.Search in Google Scholar

Rosidah Y, Yam MF, Sadikun A, Ahmad M, Akowuah GA, Asmawi MZ. (2009). Toxicology evaluation of standardized methanol extract of Gynura procumbens. J Ethnopharmacol123: 244–249.Search in Google Scholar

Rufino MSM, Alves RE, Fernandes FAN, Brito ES. (2011). Free radical scavenging behavior of ten exotic tropical fruits extracts. Food Res Int44: 2072–2075.Search in Google Scholar

Santos FJB, Moura DJ, Peres DJ, Sperotto ARM, Caramao EB, Cavalcante AACM, Saffi J. (2012). Genotoxic and mutagenic properties of Bauhinia platypetala extract, a traditional Brazilian medicinal plant. J Ethnopharmacol144: 474–482.Search in Google Scholar

Schmeiser HH, Stiborova M, Arlt VM. (2009). Chemical and molecular basis of the carcinogenicity of Aristolochia plants. Curr Opinion Drug Discovery Devept12(1): 141–148.Search in Google Scholar

Schmidt W. (1976). The micronucleus test for cytogenetic analysis. In Hollaender, A. (ed.), Chemical Mutagens, Vol. 4. Plenum Press, New York, pp. 31–53.10.1007/978-1-4684-0892-8_2Search in Google Scholar

Sowemimo AA, Fakoya FA, Awopetu I, Omobuwajo OR, Adesanya SA. (2007). Toxicity and mutagenic activity of some selected Nigerian plants. J Ethnopharmacol113(3): 427–432.Search in Google Scholar

Taylor RJ. (1980). Food Additives. John Wiley and Sons, New York.Search in Google Scholar

Tomlinson B, Chan TY, Chan JC, Critchley JA, But PP. (2000). Toxicity of complementary therapies: an eastern perspective. J Clin Pharmacol 40: 451–456.Search in Google Scholar

Uchendu CN, Isek T. (2008). Antifertility activity of aqueous ethanolic leaf extract of Spondias mombin (Anacardiaceae) in rats. Afri Health Sci8(3): 163–167.Search in Google Scholar

Ulrich-Merzenich G, Zeitler H, Jobst D, Panek D, Vetter H, Wagner H. (2007). Application of the „Omic“ technologies in phytomedicine. Phytomedicine14: 70–82.Search in Google Scholar

Van den Berg SJPL, Restani P, Boersma MG, Delmulle L, Rietjens IMCM. (2011). Levels of genotoxic and carcinogenic compounds in plant food supplements and associated risk assessment. Food Nutr Sci2: 989–1010.Search in Google Scholar

Verschaeve L, Van Staden J. (2008). Mutagenic and antimutagenic properties of extracts from South African traditional medicinal plants. J Ethnopharmacol119: 575–587.Search in Google Scholar

Wahnschaffe U, Bitsch A, Kielhorn J, Mangelsdorf I. (2005). Mutagenicity testing with transgenic mice. Part I: Comparison with the mouse bone marrow micronucleus test. J Carcinogenesis4: 3–16.Search in Google Scholar

WHO – World Health Organization (2002). Traditional Medicine Growing Needs and Potential – WHO Policy Perspectives on Medicines, No. 002, May, World Health Organization, Geneva, Switzerland.Search in Google Scholar

Wyrobek AJ, Bruce WR. (1975). Chemical induction of sperm abnormalities in mice. PNAS72: 4425–4429.Search in Google Scholar

Wyrobek AJ, Bruce WR. (1978). The induction of sperm-shape abnormalities in mice and humans. Volume 5, pp. 257–285. In: Chemical Mutagens. A. Hollander and F.J. de Serres (Eds.). Plenum Press, New York.Search in Google Scholar

Wyrobek AJ, Bruce WR. (1980). The induction of sperm shape abnormalities in mice and human, in: A. Hollander, F.J. de Serres _Eds.., Chemical Mutagens, vol. 5, Plenum, New York. pp. 257–285.Search in Google Scholar

Wyrobek AJ, Gordon LA, Burkhart JG, Francis MW, Kapp Jr RW, Letz G, Malling JC, Topham HG, Whorton MD. (1983). An evaluation of the mouse sperm morphology test and other sperm tests in non-human mammals. A report of the United States Environmental Protection Agency Gene –Tox Programme. Mutat Res115: 1–72.Search in Google Scholar

Zeiger, E. 2003. Illusions of safety: antimutagens can be mutagens, and anti-carcinogens can be carcinogens. Mutat Res543(3): 191–194.10.1016/S1383-5742(02)00111-4Search in Google Scholar

Zini A, Al-Hathal N. (2011). Antioxidant therapy in male infertility: fact or fiction? Asian J Androl13: 374–381.10.1038/aja.2010.182373933921516118Search in Google Scholar