Morphometrical and molecular data on plant parasitic nematodes Longidorus attenuatus Hooper, 1961 and L. danuvii Barsi et al., 2007 (Nematoda: Longidoridae) reported from Ukraine for the first time

Fig. 1

Data on localities, host plants and morphometrics of L_ danuvii and L_ attenuatus populations from Lviv, Ukraine_ All measurements are in μm, except for body length (in mm) and in form: mean ± standard deviation (range)

		L. danuvii		L. attenuatus
Location and hostplant (s)	Citadel parkN49.49580°; E24.01222°Acer platanoides L.	St. George’s Cathedral gardenN49.50184°; E24.00516°Malus domestica Borkh.	Lychakiv school gardenN49.50158°;E 24.03499°Malus domestica Borkh., Pyrus sp., Rubus idaeus L.	Lychakiv school gardenN49.50158°;E 24.03499°Malus domestica Borkh
Character
N	14	6	29	9
L	4.80 ± 0.34(4.08 – 5.24)	4.16 ± 0.26(3.88 – 4.46)	4.32 ± 0.28(3.81 – 5.04)	6.34 ± 0.56(5.20 – 6.95)
a	113.5 ± 5.14(103.9 – 123.9)	102.6 ± 2.18(99.8 – 105.0)	100.1 ± 5.15(89.5 – 114.4)	139.7 ± 8.49(123.8 – 151.1)
b	14.1 ± 1.45(11.0 – 17.1)	13.6 ± 1.49(12.0 – 16.3)	12.5 ± 1.96(9.0 – 16.8)	16.2 ± 1.53(13.9 – 18.5)
c	120.4 ± 12.34(90.8 – 141.7)	99.6 ± 6.98(88.2 – 108.8)	102.9 ± 7.45(87.6 – 118.5)	127.9 ± 10.63(111.9 – 144.3)
c’	1.45 ± 0.11(1.28 – 1.67)	1.59 ± 0.10(1.48 – 1.73)	1.52 ± 0.10(1.32 – 1.81)	1.53 ± 0.08(1.41 – 1.66)
d	2.0 ± 0.13(1.8 – 2.3)	2.0 ± 0.13(1.8 – 2.2)	2.2 ± 0.12(1.9 – 2.4)	2.1 ± 0.09(1.9 – 2.2)
d’	1.5 ± 0.10(1.3 – 1.6)	1.5 ± 0.12(1.4 – 1.7)	1.5 ± 0.08(1.3 – 1.7)	1.4 ± 0.09(1.3 – 1.6)
V	47.7 ± 1.64(43.9 – 49.7)	48.6 ± 1.99(45.5 – 51.0)	49.2 ± 1.32(46.2 – 52.7)	49.1 ± 1.45(47.5 – 52.2)
Odontostylet length	87.1 ± 2.2(81 – 90)	90.8 ± 0.8(90 – 92)	90.2 ± 2.12(86 – 95)	82.7 ± 2.3(78 – 85)
Odontophore length	50.9 ± 2.4(45 – 54)	54.0 ± 1.3(53 – 56)	56.6 ± 3.57(49 – 63)	59.4 ± 2.4(56 – 63)
Total stylet length	137.9 ± 3.5(130 – 143)	144.8 ± 1.7(143 – 147)	146.8 ± 4.45(136 – 155)	142.1 ± 3.6(137 – 147)
Anterior end to guide ring	26.1 ± 0.9(24 – 27)	26.3 ± 1.0(25 – 28)	27.9 ± 1.05(26 – 30)	29.9 ± 1.2(28 – 31)
Pharyngeal bulb length	80.0 ± 3.6(72 – 86)	81.3 ± 2.0(78 – 83)	86.5 ± 6.71(73 – 99)	112.2 ± 4.6(105 – 121)
Pharyngeal bulb width	16.1 ± 1.7(13 – 19)	15.7 ± 0.8(15 – 17)	17.5 ± 1.27(15 – 20)	18.3 ± 1.4(16 – 21)
Tail length	40.1 ± 2.6(37 – 45)	41.8 ± 2.6(38 – 45)	42.1 ± 2.71(37 – 48)	49.7 ± 3.8(44 – 56)
Hyaline part of tail length	9.6 ± 1.2(8 – 12)	10.5 ± 1.4(8 – 12)	10.0 ± 1.09(8 – 12)	13.3 ± 2.0(9 – 16)
Width at level of:
lips	12.9 ± 0.6(12 – 14)	13.2 ± 0.8(12 – 14)	12.9 ± 0.54(12 – 14)	14.3 ± 0.5(14 – 15)
guide ring	18.9 ± 1.1(17 – 21)	19.2 ± 0.8(18 – 20)	18.8 ± 0.62(18 – 20)	20.2 ± 1.0(19 – 22)
base of pharynx	36.7 ± 1.7(33 – 39)	34.7 ± 1.5(32 – 36)	35.8 ± 1.36(32 – 38)	38.9 ± 1.1(37 – 40)
vulva	42.4 ± 3.0(37 – 47)	40.5 ± 1.9(38 – 43)	43.2 ± 1.97(40 – 47)	45.3 ± 1.9(42 – 48)
anus	27.6 ± 1.3(25 – 29)	26.3 ± 0.8(25 – 27)	27.7 ± 1.03(26 – 30)	32.6 ± 1.7(29 – 35)

Data on morphometrics of Longidorus danuvii juveniles from Citadel Park, Lviv, Ukraine_ All measurements are in μm, except for body length (in mm) and in form: mean ± standard deviation (range)_

Character	J1	J2	J3	J4
n	3	8	16	13
L	1.21(1.18 – 1.29)	1.66 ± 0.07(1.56 – 1.75)	2.09 ± 0.20(1.71 – 2.42)	3.28 ± 0.30(2.70 – 3.79)
a	54.1(53.0 – 55.9)	67.4 ± 4.06(59.9 – 72.0)	70.7 ± 3.87(62.2 – 77.6)	98.9 ± 6.14(97.0 – 111.5)
b	6.8(5.5 – 8.8)	6.1 ± 0.41(5.3 – 6.7)	7.6 ± 1.58(5.4 – 10.5)	9.9 ± 0.82(8.5 – 11.0)
c	32.1(29.9 – 35.6)	42.7 ± 2.63(39.2 – 47.3)	48.2 ± 6.02(36.4 – 56.9)	76.9 ± 8.54(62.7 – 88.9)
c’	2.43(2.2 – 2.6)	2.30 ± 0.28(2.06 – 2.93)	2.11 ± 0.27(1.86 – 2.40)	1.75 ± 0.15(1.5 – 2.0)
d	1.9(1.8 – 2.0)	1.9 ± 0.10(1.8 – 2.1)	2.1 ± 0.15(1.8 – 2.3)	2.0 ± 0.12(1.8 – 2.2)
d’	1.4(1.3 – 1.6)	1.5 ± 0.13(1.3 – 1.7)	1.5 ± 0.10(1.4 – 1.8)	1.5 ± 0.08(1.3 – 1.6)
Odontostylet length	53.3(53 – 54)	60.4 ± 0.9(59 – 61)	69.3 ± 2.0(65 – 72)	76.1 ± 1.3(73 – 78)
Odontophore length	–	43.9 ± 2.6(39 – 47)	45.7 ± 2.1(42 – 49)	49.0 ± 2.8(43 – 53)
Total stylet length	–	104.3 ± 3.0(98 – 107)	114.9 ± 2.7(111 – 121)	125.2 ± 3.4(120 – 130)
Replacement odontostylet length	61(60 – 63)	68.8 ± 3.0(62 – 71)	77.9 ± 2.2(74 – 82)	84.8 ± 2.2(81 – 88)
Anterior end to guide ring	16	18.4 ± 1.1(17 – 20)	21.1 ± 0.7(20 – 22)	23.2 ± 0.8(22 – 24)
Pharyngeal bulb length	48.7(43 – 52)	57.0 ± 3.1(54 – 64)	64.8 ± 4.8(57 – 73)	76.2 ± 4.4(71 – 83)
Pharyngeal bulb width	10.7(9 – 12)	12.8 ± 1.0(11 – 14)	13.4 ± 0.8(13 – 15)	15.5 ± 1.1(13 – 17)
Tail length	37.3(33 – 40)	38.9 ± 2.2(37 – 42)	43.6 ± 2.4(39 – 48)	42.8 ± 3.2(38 – 49)
Hyaline part of tail length	5.3(5 – 6)	5.9 ± 0.6(5 – 7)	7.4 ± 1.0(6 – 9)	8.5 ± 1.1(7 – 11)
Width at level of:
lips	8.3(8 – 9)	9.5 ± 0.5(9 – 10)	10.2 ± 0.8(9 – 12)	11.6 ± 0.5(11 – 12)
guide ring	12(11 – 13)	14.4 ± 1.1(13 – 16)	15.6 ± 0.6(14 – 16)	17.2 ± 1.0(15 – 18)
base of pharynx	21.3(21 – 22)	23 ± 1.1(22 – 25)	27.4 ± 1.2(25 – 29)	31.5 ± 1.3(29 – 34)
mid-body	22.3(22 – 23)	24.6 ± 1.1(23 – 26)	29.6 ± 2.4(26 – 34)	33.2 ± 1.3(31 – 35)
anus	15.3(15 – 16)	17 ± 1.3(14 – 18)	20.7 ± 0.8(19 – 22)	24.5 ± 1.3(22 – 26)