<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Enterobiasis is a human intestinal parasitic disease caused by human pinworm, <italic>Enterobius vermicularis</italic>. Despite being the most prevalent nematode infection in Europe and North America, predominantly among in school aged children, the data concerning infection rate and knowledge of genetic variability of pinworms are incomplete. The aim of the study was the estimation of prevalence and molecular typing of <italic>Enterobius vermicularis</italic> among healthy children in north-eastern Poland. In 2013 – 2015, 296 individuals (aged 2 – 18 years) from 12 kindergartens, schools and orphanages were examined by the adhesive cellophane tape method. Data on socio-demographic status were collected using a questionnaire. Molecular analysis was performed using the DNA of adult female pinworms and primers targeting the region of cytochrome oxidase I gene. The overall prevalence of enterobiasis was 10.1 %. <italic>Enterobius vermicularis</italic> infection rates were 3.9 % in children living in families and 32.8 % among the orphans (OR=0.08; 95 % CI: 0.04 – 0.19; p&lt;0.001). There were no associations between distribution of enterobiasis and gender, pets possession and the season of examination. In 43.3 % of the infected children enterobiasis was asymptomatic. Based on a molecular marker three different haplotypes of pinworm were identified. All sequences clustered within type B, together with human <italic>E. vermicularis</italic> isolates from Denmark, Germany, Greece, and Japan. This paper provides complementary data on the occurrence and intraspecific variability of <italic>E. vermicularis</italic> in human population in Europe.</p></abstract>

Enterobiasis is a human intestinal parasitic disease caused by human pinworm, Enterobius vermicularis. Despite being the most prevalent nematode infection in Europe and North America, predominantly among in school aged children, the data concerning infection rate and knowledge of genetic variability of pinworms are incomplete. The aim of the study was the estimation of prevalence and molecular typing of Enterobius vermicularis among healthy children in north-eastern Poland. In 2013 – 2015, 296 individuals (aged 2 – 18 years) from 12 kindergartens, schools and orphanages were examined by the adhesive cellophane tape method. Data on socio-demographic status were collected using a questionnaire. Molecular analysis was performed using the DNA of adult female pinworms and primers targeting the region of cytochrome oxidase I gene. The overall prevalence of enterobiasis was 10.1 %. Enterobius vermicularis infection rates were 3.9 % in children living in families and 32.8 % among the orphans (OR=0.08; 95 % CI: 0.04 – 0.19; p<0.001). There were no associations between distribution of enterobiasis and gender, pets possession and the season of examination. In 43.3 % of the infected children enterobiasis was asymptomatic. Based on a molecular marker three different haplotypes of pinworm were identified. All sequences clustered within type B, together with human E. vermicularis isolates from Denmark, Germany, Greece, and Japan. This paper provides complementary data on the occurrence and intraspecific variability of E. vermicularis in human population in Europe.

Enterobiasis epidemiology and molecular characterization of <italic>Enterobius vermicularis</italic> in healthy children in north-eastern Poland

Department of Medical Biology, Faculty of Health Sciences

University of Warmia and Mazury in Olsztyn

Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology

Helminthologia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enterobiasis epidemiology and molecular characterization of  in healthy children in north-eastern Poland

Enterobiasis is a human intestinal parasitic disease caused by human pinworm, Enterobius vermicularis. Despite being the most prevalent nematode infection in...

Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

Online veröffentlicht: 01. Nov. 2017

Seitenbereich: 284 - 291

Eingereicht: 24. Feb. 2017

Akzeptiert: 20. Juni 2017

DOI: https://doi.org/10.1515/helm-2017-0042

Schlüsselwörter
enterobiasis, children, genotyping, Poland

© 2017 K. Kubiak et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

Online veröffentlicht: 01. Nov. 2017

Seitenbereich: 284 - 291

Eingereicht: 24. Feb. 2017

Akzeptiert: 20. Juni 2017

DOI: https://doi.org/10.1515/helm-2017-0042

Schlüsselwörterenterobiasis, children, genotyping, Poland

© 2017 K. Kubiak et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
enterobiasis, children, genotyping, Poland