<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The present investigation was to estimate the total phenolic content and composition of flavonoids and hydroxycinnamic acids in herbal infusions obtained from aerial parts of three <italic>Lamiaceae</italic> species (<italic>Dracocephalum moldavica, Ocimum americanum</italic> and <italic>Satureja hortensis</italic>). The total phenolic content of herbal infusions was determined using a spectrophotometric method, whereas the individual phenolics were assessed by high-performance liquid chromatography (HPLC). The HPLC method was developed and validated. The total phenolic content was measured by applying the Folin-Ciocalteu method with reference to gallic acid. Results were in the range from 29.39 to 65.38 mg estimated as gallic acid equivalents per gram of dry herb. The phenolic profile was, in turn, analysed by HPLC and consisted of gallic acid, hydroxycinnamic acids (caffeic, chlorogenic, ferulic, and rosmarinic) and flavonoids (rutin, hyperoside, quercitrin, quercetin, apigenin, apigenin-7-glucoside and catechin) in different concentrations. Rosmarinic acid was the predominant component among the hydroxycinnamic acids in herbal infusions of all three plants. This was found to be in the range of 3.64 to 5.28 mg per gram of dry herb. Apigenin-7-glucoside, quercitrin and hyperoside were the prevailing flavonoid components of the infusions.</p></abstract>

The present investigation was to estimate the total phenolic content and composition of flavonoids and hydroxycinnamic acids in herbal infusions obtained from aerial parts of three Lamiaceae species (Dracocephalum moldavica, Ocimum americanum and Satureja hortensis). The total phenolic content of herbal infusions was determined using a spectrophotometric method, whereas the individual phenolics were assessed by high-performance liquid chromatography (HPLC). The HPLC method was developed and validated. The total phenolic content was measured by applying the Folin-Ciocalteu method with reference to gallic acid. Results were in the range from 29.39 to 65.38 mg estimated as gallic acid equivalents per gram of dry herb. The phenolic profile was, in turn, analysed by HPLC and consisted of gallic acid, hydroxycinnamic acids (caffeic, chlorogenic, ferulic, and rosmarinic) and flavonoids (rutin, hyperoside, quercitrin, quercetin, apigenin, apigenin-7-glucoside and catechin) in different concentrations. Rosmarinic acid was the predominant component among the hydroxycinnamic acids in herbal infusions of all three plants. This was found to be in the range of 3.64 to 5.28 mg per gram of dry herb. Apigenin-7-glucoside, quercitrin and hyperoside were the prevailing flavonoid components of the infusions.

Phenolic compounds of herbal infusions obtained from some species of the <italic>Lamiaceae</italic> family

Department of Pharmacognosy and Medical Botany

I. Horbachevsky Ternopil State Medical University

State Laboratory for Quality Control of Medicines

Institute of Pharmacology and Toxicology of NAMS of Ukraine

Department of the Chemistry of Sulfurorganic Compounds

Laboratory of Condensed Heterocyclic Systems

Department of Drug Technology and Biopharmaceutics

Danylo Halytsky Lviv National Medical University

Department of Analytical and Ecological Chemistry

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phenolic compounds of herbal infusions obtained from some species of the  family

The present investigation was to estimate the total phenolic content and composition of flavonoids and hydroxycinnamic acids in herbal infusions obtained from...

Phenolic compounds of herbal infusions obtained from some species of the Lamiaceae family

Online veröffentlicht: 03. Jan. 2019

Seitenbereich: 194 - 199

Eingereicht: 28. Feb. 2018

Akzeptiert: 08. Juni 2018

DOI: https://doi.org/10.1515/cipms-2018-0036

Schlüsselwörter
Dracocephalum moldavica, Ocimum americanum, Satureja hortensis, phenolic compounds, HPLC, spectrophotometry

© 2018 Mariia Shanaida et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Phenolic compounds of herbal infusions obtained from some species of the Lamiaceae family

Online veröffentlicht: 03. Jan. 2019

Seitenbereich: 194 - 199

Eingereicht: 28. Feb. 2018

Akzeptiert: 08. Juni 2018

DOI: https://doi.org/10.1515/cipms-2018-0036

SchlüsselwörterDracocephalum moldavica, Ocimum americanum, Satureja hortensis, phenolic compounds, HPLC, spectrophotometry

© 2018 Mariia Shanaida et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
Dracocephalum moldavica, Ocimum americanum, Satureja hortensis, phenolic compounds, HPLC, spectrophotometry