<abstract xmlns="http://www.w3.org/1999/xhtml"><p><italic>Streptococcus iniae</italic> is a causative agent of hemorrhagic septicemia in olive flounder, <italic>Paralichthys olivaceus</italic>, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan) was prepared from the <italic>S. iniae</italic> F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (−) and SI-36 38 (+). The vaccine recognized both serotypes. It showed a very high effective immune response against <italic>S. iniae</italic>; the challenge test using the <italic>S. iniae</italic> SI-36 strain resulted in a relative percent survival (RPS) of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with <italic>S. iniae</italic> SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against <italic>S. iniae</italic> and could be applied in commercial aquafarms as a therapeutic agent against β-hemolytic streptococcosis in cultured <italic>P. olivaceus</italic>.</p></abstract>

Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses. As a preventive measure, M VAC INIAE (Mastuken, Japan) was prepared from the S. iniae F2K strain and tested against the SI-36 strain prevalent on flounder fish farms on Jeju Island, Korea. F2K had a serotype of 38 (−) and SI-36 38 (+). The vaccine recognized both serotypes. It showed a very high effective immune response against S. iniae; the challenge test using the S. iniae SI-36 strain resulted in a relative percent survival (RPS) of 85.7-87.0% 2 weeks after vaccination and 71.0-80.0% 6 months after vaccination. Field vaccination and clinical challenge tests were performed at local Jeju aquafarms with S. iniae SI-36. These showed significantly reduced cumulative mortality when compared to the control group with RPS rates that ranged between 71-80%. Hence, the present study suggests that this vaccine showed a significant immune response against S. iniae and could be applied in commercial aquafarms as a therapeutic agent against β-hemolytic streptococcosis in cultured P. olivaceus.

Protective efficiency of an inactivated vaccine against <italic>Streptococcus iniae</italic> in olive flounder, <italic>Paralichthys olivaceus</italic>

Marine Pathogenic Microbes Lab, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju 690-756, South Korea

Jeju Special Self-Governing Province Ocean and Fisheries Research Institute, Pyoseon-myeon, Segwipo-si, Jeju 697-914, South Korea

Department of Aquatic Life Medicine, Kunsan National University, Kunsan 573-701, South Korea

Marine and Environmental Institute, Jeju National University, Jeju 690-814, South Korea

Fisheries & Aquatic Life

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Protective efficiency of an inactivated vaccine against  in olive flounder, 

Streptococcus iniae is a causative agent of hemorrhagic septicemia in olive flounder, Paralichthys olivaceus, in Korea, resulting in serious economic losses....

Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

Online veröffentlicht: 09. Apr. 2016

Seitenbereich: 23 - 32

Eingereicht: 22. Sept. 2015

Akzeptiert: 07. März 2016

DOI: https://doi.org/10.1515/aopf-2016-0003

Schlüsselwörter
β-hemolytic streptococcosis, olive flounder, inactivated vaccine, relative percent survival

© 2016 Archives of Polish Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Protective efficiency of an inactivated vaccine against Streptococcus iniae in olive flounder, Paralichthys olivaceus

Online veröffentlicht: 09. Apr. 2016

Seitenbereich: 23 - 32

Eingereicht: 22. Sept. 2015

Akzeptiert: 07. März 2016

DOI: https://doi.org/10.1515/aopf-2016-0003

Schlüsselwörterβ-hemolytic streptococcosis, olive flounder, inactivated vaccine, relative percent survival

© 2016 Archives of Polish Fisheries

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
β-hemolytic streptococcosis, olive flounder, inactivated vaccine, relative percent survival