<abstract xmlns="http://www.w3.org/1999/xhtml"><p> Marbling characterized by the amount and distribution of intramuscular fat (IMF) in longissimus muscle (LM) and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. The myosin binding protein C, slow type (MYBPC1) gene, involved in efficient energy metabolism and homeostasis during muscle contraction in slow skeletal muscle, has been previously shown to be expressed at different levels in the LM between high-marbled and lowmarbled steer groups using differential-display PCR (ddPCR). In this study, we found that IMF area (%) in the sacrococcygeus muscle (SM) was positively correlated with BMS in the LM in Japanese Black steers (n=22, r=0.941, P&lt;0.0001). This suggested that the IMF area (%) in the SM tends to equate marbling level in the LM. Furthermore, we showed that the MYBPC1 expression level in SM was significantly higher in the Japanese Black steers (n=5) with high BMS than in the Japanese Black steers (n=5) with low BMS (P&lt;0.001). Moreover, correlation analyses showed that the expression level of the MYBPC1 gene was positively correlated with IMF area (%) (n=22, r=0.858, P&lt;0.0001) and BMS (n=22, r=0.769, P&lt;0.0001), indicating the association of MYBPC1 expression level with marbling trait. These results, together with the previous ddPCR result, suggested that high level of MYBPC1 expression may be associated with the development of marbling in Japanese Black beef cattle.</p></abstract>

Marbling characterized by the amount and distribution of intramuscular fat (IMF) in longissimus muscle (LM) and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. The myosin binding protein C, slow type (MYBPC1) gene, involved in efficient energy metabolism and homeostasis during muscle contraction in slow skeletal muscle, has been previously shown to be expressed at different levels in the LM between high-marbled and lowmarbled steer groups using differential-display PCR (ddPCR). In this study, we found that IMF area (%) in the sacrococcygeus muscle (SM) was positively correlated with BMS in the LM in Japanese Black steers (n=22, r=0.941, P<0.0001). This suggested that the IMF area (%) in the SM tends to equate marbling level in the LM. Furthermore, we showed that the MYBPC1 expression level in SM was significantly higher in the Japanese Black steers (n=5) with high BMS than in the Japanese Black steers (n=5) with low BMS (P<0.001). Moreover, correlation analyses showed that the expression level of the MYBPC1 gene was positively correlated with IMF area (%) (n=22, r=0.858, P<0.0001) and BMS (n=22, r=0.769, P<0.0001), indicating the association of MYBPC1 expression level with marbling trait. These results, together with the previous ddPCR result, suggested that high level of MYBPC1 expression may be associated with the development of marbling in Japanese Black beef cattle.

Association of the Expression Level of the MYBPC1 Gene in Skeletal Muscle With Marbling Trait in Japanese Black Beef Cattle

Faculty of Agriculture, Department of Agrobiology, Niigata University, 950-2181 Niigata, Japan

Faculty of Health Sciences for Welfare, Department of Nutritional Sciences for Well-being, Kansai University of Welfare Sciences, 582-0026 Osaka, Japan

Central Pharmaceutical Research Institute, Japan Tobacco, Inc., 569-1125 Osaka, Japan

Department of Life Science, Division of Natural Sciences, International Christian University, 181-8585 Tokyo, Japan

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Marbling characterized by the amount and distribution of intramuscular fat (IMF) in longissimus muscle (LM) and measured as beef marbling score (BMS), is an...

Association of the Expression Level of the MYBPC1 Gene in Skeletal Muscle With Marbling Trait in Japanese Black Beef Cattle

Online veröffentlicht: 23. Apr. 2015

Seitenbereich: 349 - 358

Eingereicht: 05. Okt. 2014

Akzeptiert: 05. Jan. 2015

DOI: https://doi.org/10.1515/aoas-2015-0014

Schlüsselwörter
intramuscular fat, Japanese Black breed, marbling, MYBPC1, skeletal muscle

© by Bin Tong

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Association of the Expression Level of the MYBPC1 Gene in Skeletal Muscle With Marbling Trait in Japanese Black Beef Cattle

Online veröffentlicht: 23. Apr. 2015

Seitenbereich: 349 - 358

Eingereicht: 05. Okt. 2014

Akzeptiert: 05. Jan. 2015

DOI: https://doi.org/10.1515/aoas-2015-0014

Schlüsselwörterintramuscular fat, Japanese Black breed, marbling, MYBPC1, skeletal muscle

© by Bin Tong

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
intramuscular fat, Japanese Black breed, marbling, MYBPC1, skeletal muscle