<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Arylsulfatase A (ARSA) is a lysosomal enzyme that plays an important role in catalysis of degradation of cerebrosidesulphate. The deficiency of this lysosomal enzyme causes an autosomal recessive disorder, called metachromatic leucodystrophy. However, a low ARSA activity can be observed in clinically healthy people, called ARSA pseudodeficiency. In our study we investigated the possible linkage between ARSA activity and sulfatide deficiency causing characteristic aspects of degenerative diseases, such as end stage kidney disease, type 2 Diabetes mellitus, Parkinson syndrome, prostate cancer and HIV (Human Immunodeficiency Virus) infection. We used a spectrophotometric method to determine the activity of ARSA. This method of enzyme dosage is based on a 4 hour long hydrolysis of the ARSA enzyme on 4-nitrocatechol sulfate (p-NCS) substrate. The unit of this measurement is nmol/ml/4h. Our findings show significant values in type 2 diabetes, Parkinson syndrome and chronic kidney disease. The importance of sulfatide in these diseases is well-known, thus presumably the variation of the ARSA’s activity might play an important role in the pathophysiology of these diseases, involving a vicious cycle between sulfatide degradation andthese diseases.</p></abstract>

Arylsulfatase A (ARSA) is a lysosomal enzyme that plays an important role in catalysis of degradation of cerebrosidesulphate. The deficiency of this lysosomal enzyme causes an autosomal recessive disorder, called metachromatic leucodystrophy. However, a low ARSA activity can be observed in clinically healthy people, called ARSA pseudodeficiency. In our study we investigated the possible linkage between ARSA activity and sulfatide deficiency causing characteristic aspects of degenerative diseases, such as end stage kidney disease, type 2 Diabetes mellitus, Parkinson syndrome, prostate cancer and HIV (Human Immunodeficiency Virus) infection. We used a spectrophotometric method to determine the activity of ARSA. This method of enzyme dosage is based on a 4 hour long hydrolysis of the ARSA enzyme on 4-nitrocatechol sulfate (p-NCS) substrate. The unit of this measurement is nmol/ml/4h. Our findings show significant values in type 2 diabetes, Parkinson syndrome and chronic kidney disease. The importance of sulfatide in these diseases is well-known, thus presumably the variation of the ARSA’s activity might play an important role in the pathophysiology of these diseases, involving a vicious cycle between sulfatide degradation andthese diseases.

Arylsulfatase A: An Important Metabolic Factor in Pathophysiology of Different Diseases

Department of Biochemistry, University of Medicine and Pharmacy Tîrgu Mureș, Romania

Acta Marisiensis - Seria Medica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Arylsulfatase A (ARSA) is a lysosomal enzyme that plays an important role in catalysis of degradation of cerebrosidesulphate. The deficiency of this lysosomal...

Arylsulfatase A: An Important Metabolic Factor in Pathophysiology of Different Diseases

Online veröffentlicht: 07. Nov. 2015

Seitenbereich: 233 - 235

Eingereicht: 09. Dez. 2014

Akzeptiert: 18. Juli 2015

DOI: https://doi.org/10.1515/amma-2015-0064

Schlüsselwörter
arylsulfatase A, sulfatide, chronic kidney disease, type 2 diabetes, Parkinson syndrome, prostate cancer, HIV infection

© 2015 Kovacs Zsolt et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Arylsulfatase A: An Important Metabolic Factor in Pathophysiology of Different Diseases

Online veröffentlicht: 07. Nov. 2015

Seitenbereich: 233 - 235

Eingereicht: 09. Dez. 2014

Akzeptiert: 18. Juli 2015

DOI: https://doi.org/10.1515/amma-2015-0064

Schlüsselwörterarylsulfatase A, sulfatide, chronic kidney disease, type 2 diabetes, Parkinson syndrome, prostate cancer, HIV infection

© 2015 Kovacs Zsolt et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Schlüsselwörter
arylsulfatase A, sulfatide, chronic kidney disease, type 2 diabetes, Parkinson syndrome, prostate cancer, HIV infection