<abstract xmlns="http://www.w3.org/1999/xhtml"> The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life.</abstract>

The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life.

Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves

Department of Physiology and Biochemistry, Faculty of Veterinary Medicine University of Belgrade, Belgrade, Serbia

Department of Physiology, Faculty of Veterinary Medicine, University St Kliment Ohridski-Bitola, Bitola, FYROM

Faculty of Agriculture, University of Banja Luka, Banja Luka, Republic of Srpska, Bosnia and Herzegovina

Department of Farm Animal Diseases, Faculty of Veterinary Medicine University of Belgrade, Belgrade, Serbia

Department of Economic and Statistics, Faculty of Veterinary Medicine University of Belgrade, Belgrade, Serbia

Veterinary Station “Veterina system Sladojević”, Gradiška, Republic of Srpska, Bosnia and Herzegovina

Department of Biochemistry, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Belgrade, Serbia

Acta Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

The aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included...

Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves

Online veröffentlicht: 23. März 2016

Seitenbereich: 61 - 75

Eingereicht: 15. Okt. 2015

Akzeptiert: 11. Dez. 2015

DOI: https://doi.org/10.1515/acve-2016-0005

Schlüsselwörter
cows, placental deiodinases, neonate, thyroid axis, PTU

© by Danijela Kirovski

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves

Online veröffentlicht: 23. März 2016

Seitenbereich: 61 - 75

Eingereicht: 15. Okt. 2015

Akzeptiert: 11. Dez. 2015

DOI: https://doi.org/10.1515/acve-2016-0005

Schlüsselwörtercows, placental deiodinases, neonate, thyroid axis, PTU

© by Danijela Kirovski

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Schlüsselwörter
cows, placental deiodinases, neonate, thyroid axis, PTU