Uneingeschränkter Zugang

Lactobacillus plantarum B7 attenuates Salmonella typhimurium infection in mice: preclinical study in vitro and in vivo

Siwaporn Wongsen
Siwaporn Wongsen
, 
Duangporn Werawatganon
Duangporn Werawatganon
 und 
Somying Tumwasorn
Somying Tumwasorn
   | 01. Okt. 2019
Asian Biomedicine's Cover Image
Über diesen Artikel
Vorheriger Artikel
Nächster Artikel
Zitieren
Article Category: Original article
Online veröffentlicht: 01. Okt. 2019
Seitenbereich: 211 - 218
DOI: https://doi.org/10.1515/abm-2019-0022
Schlüsselwörter
© 2018 Siwaporn Wongsen et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Figure 1

L. plantarum B7 spots grown on 20 mM glucose BHI agar under anaerobic conditions at 37ºC, 48 h, overlaid with 107S. Typhimurium and incubated at 37ºC, 24 h, under aerobic conditions. Clear zone (C) and microcolonies (M) around the spot (n = 6).
L. plantarum B7 spots grown on 20 mM glucose BHI agar under anaerobic conditions at 37ºC, 48 h, overlaid with 107S. Typhimurium and incubated at 37ºC, 24 h, under aerobic conditions. Clear zone (C) and microcolonies (M) around the spot (n = 6).

Figure 2

Concentration of S. typhimurium in 1 g feces (logCFU/g). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05
Concentration of S. typhimurium in 1 g feces (logCFU/g). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05

Figure 3

Concentration of serum TNF-α (pg/mL). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05
Concentration of serum TNF-α (pg/mL). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05

Figure 4

Concentration of serum IL-6 (pg/mL). Control group (n = 8): mice administered with 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05.
Concentration of serum IL-6 (pg/mL). Control group (n = 8): mice administered with 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05.

Figure 5

Concentration of serum CXCL1 (pg/mL). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05
Concentration of serum CXCL1 (pg/mL). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. typhimurium 3 × 109 CFU/mL. *P < 0.05

Figure 6

Fecal characteristics of mice in all groups (n = 24). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice fed with L. plantarum B7 1 × 108CFU/mL and S. typhimurium 3 × 109 CFU/mL.
Fecal characteristics of mice in all groups (n = 24). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice fed with L. plantarum B7 1 × 108CFU/mL and S. typhimurium 3 × 109 CFU/mL.

Figure 7

Percentage of feces moisture content (%FMC) in all groups (mean ± SD). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. Typhimurium 3 × 109 CFU/mL. *P < 0.05
Percentage of feces moisture content (%FMC) in all groups (mean ± SD). Control group (n = 8): mice administered 0.85% saline; S group (n = 8): mice administered S. typhimurium 3 × 109 CFU/mL; S + LP group (n = 8): mice administered L. plantarum B7 1 × 108 CFU/mL and S. Typhimurium 3 × 109 CFU/mL. *P < 0.05
eISSN:
1875-855X
Sprache:
Englisch
Zeitrahmen der Veröffentlichung:
6 Hefte pro Jahr
Fachgebiete der Zeitschrift:
Medizin, Gesundheitsfachberufe, Vorklinische Medizin, Grundlagenmedizin, andere, Klinische Medizin
Zeitschrift RSS Feed