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Polish Journal of Microbiology
Volume 67 (2018): Issue 1 (January 2018)
Open Access
Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in
Bacteroides
Species
Isin Akyar
Isin Akyar
,
Meltem Ayas
Meltem Ayas
,
Onur Karatuna
Onur Karatuna
and
Yesim Besli
Yesim Besli
| Mar 09, 2018
Polish Journal of Microbiology
Volume 67 (2018): Issue 1 (January 2018)
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Article Category:
short-communication
Published Online:
Mar 09, 2018
Page range:
97 - 101
Received:
Oct 19, 2016
Accepted:
Dec 28, 2017
DOI:
https://doi.org/10.5604/pjm-2018-6148
Keywords
carbapenemase
,
Carba NP
,
imipenem
© 2021 Isin Akyar et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1.
Interpretation of the Carba NP test results: B. fragilis ATCC 25285 with no carbapenemase activity (left) and carbapenemase-producing B. fragilis strain TAL 2480 (right).The tubes denoted as “a” contain solution A + bacterial lysate, tubes “b” contain solution B (solution A + imipenem) + bacterial lysate. If acidification occurs as a result of hydrolysis of imipenem, the pH indicator (phenol red) causes a color change from red to yellow in tube b.
Fig. 2.
The PCR amplification results for the cfiA gene in clinical Bacteroides spp. isolates.Lanes 1, 3 and 4 are clinical B. fragilis isolates demonstrating the presence of the cfiAX gene, lanes 2, 5–10 are clinical Bacteroides spp. lacking the cfia gene, lane 11 is negative control (water), lane 12 is a cfiA positive B. fragilis strain used as positive control. Molecular sizes in base pair (bp) are indicated at the left. (M: Molecular mass standard) This PCR assay was used to amplify a 302 bp fragment of the cfiA gene.