1. bookVolume 66 (2017): Issue 4 (December 2017)
Journal Details
License
Format
Journal
eISSN
2544-4646
First Published
04 Mar 1952
Publication timeframe
4 times per year
Languages
English
access type Open Access

Inhibition of Multidrug-Resistant Gram-Positive and Gram-Negative Bacteria by a Photoactivated Porphyrin

Published Online: 04 Dec 2017
Volume & Issue: Volume 66 (2017) - Issue 4 (December 2017)
Page range: 533 - 536
Received: 21 Nov 2016
Accepted: 12 Jan 2017
Journal Details
License
Format
Journal
eISSN
2544-4646
First Published
04 Mar 1952
Publication timeframe
4 times per year
Languages
English
Abstract

The authors studied the in vitro antibacterial activity of the photo-activated porphyrin meso-tri(N-methyl-pyridyl), mono(N-tetradecylpyridyl)porphine (C14) against four multidrug-resistant bacteria: Staphylococcus aureus, Enterococcus faecalis (Gram-positive), Escherichia coli, Pseudomonas aeruginosa (Gram-negative). Using 10 μg/ml of porphyrin and 60 sec irradiation we observed the remarkable susceptibility of S. aureus and E. faecalis to treatment while, under the same conditions, E. coli and P. aeruginosa showed very low susceptibility. In a later stage, suspensions of Gram-negative bacteria were processed with EDTA before photo-activation, obtaining a significant decrease in viable counts. In view of the results, if the combination of low porphyrin concentrations and short irradiation times will be effective in vivo also, this approach could be a possible alternative to antibiotics, in particular against localized infections due to multidrug-resistant microorganisms.

Keywords

Dai T., Y. Huang and M. Hamblin. 2009. Photodynamic therapy for localized infections-State of the art. Photodiagnosis Photodyn. Ther. 6: 170–18810.1016/j.pdpdt.2009.10.008 Search in Google Scholar

Dantes R., Y. Mu, R. Belflower, D. Aragon, G. Dumyati, L.H. Harrison, F.C. Lessa, R. Lynfield J., Nadle, S. Petit et al. 2013. National burden of invasive methicillin-resistant Staphylococcus aureus infections, United States. JAMA Intern. Med. 173: 1970–1978. Search in Google Scholar

de Niederhausern S., M. Bondi, P. Messi, R. Iseppi, C. Sabia, G. Manicardi and I. Anacarso. 2011. Vancomycin-resistance transferability from VanA enterococci to Staphylococcus aureus. Curr. Microbiol. 62: 1363–1367.10.1007/s00284-011-9868-6 Search in Google Scholar

de Niederhausern S., C. Sabia, P. Messi, E. Guerrieri, G. Manicardi and M. Bondi. 2007. VanA-type vancomycin-resistant enterococci in equine and swine rectal swabs and in human clinical samples. Curr. Microbiol. 55: 240–246.10.1007/s00284-007-0115-0 Search in Google Scholar

Huang L., T. Dai and M.R. Hamblin. 2010. Antimicrobial photodynamic inactivation and photodynamic therapy for infections. Methods Mol. Biol. 635: 155–173.10.1007/978-1-60761-697-9_12 Search in Google Scholar

Jori G., C. Fabris, M. Soncin, S. Ferro, O. Coppellotti, D. Dei, L. Fantetti, G. Chiti and G. Roncucci. 2006. Photodynamic therapy in the treatment of microbial infections: Basic principles and perspective applications. Lasers Surg. Med. 38: 468–481.10.1002/lsm.20361 Search in Google Scholar

Lazzeri D., M. Rovera, L. Pascual and E.N. Durantini. 2004. Photodynamic studies and photoinactivation of Escherichia coli using meso-substituted cationic porphyrin derivatives with asymmetric charge distribution. J. Photochem. Photobiol. 80: 286–293.10.1562/2004-03-08-RA-105.1 Search in Google Scholar

Magaraggia M., F. Faccenda, A. Gandolfi and G. Jori. 2006. Treatment of microbiologically polluted aquaculture waters by a novel photochemical technique of potentially low environmental impact. J. Environ. Monitor 8: 923–931.10.1039/b606975d Search in Google Scholar

Merchat M., G. Bertolini, P. Giacomini, A. Villanueva and G. Jori. 1996a. Meso-substituted cationic porphyrins as efficient photosensitizers of Gram-positive and Gram-negative bacteria J. Photochem. Photobiol. 32: 153–157.10.1016/1011-1344(95)07147-4 Search in Google Scholar

Merchat M., J.D. Spikes, G. Bertoloni and G. Jori. 1996b. Studies on the mechanism of bacteria photosensitization by meso-substituted cationic porphyrins. J. Photochem. Photobiol. 35: 149–153.10.1016/S1011-1344(96)07321-6 Search in Google Scholar

National Nosocomial Infections Surveillance System. 2004. National Nosocomial Infections Surveillance (NNIS) System report, data summary from January 1992 through June 2004, issued October 2004. Am. J. Infect. Control. 32: 470–485.10.1016/j.ajic.2004.10.001 Search in Google Scholar

Reddi E., M. Ceccon, G. Valduga, G. Jori, J.C. Bommer, F. Elisei, L. Latterini and U. Mazzucato. 2002. Photophysical properties and antibacterial activity of meso-substituted cationic porphyrins. Photochem. Photobiol. 75: 462–470.10.1562/0031-8655(2002)075<0462:PPAAAO>2.0.CO;2 Search in Google Scholar

Shaikh S., J. Fatima, S. Shakil, S.M.D. Rizvi and M.A. Kamal. 2015. Antibiotic resistance and extended spectrum beta-lactamases: types, epidemiology and treatment. Saudi J. Biol. Sci. 22: 90–101. Search in Google Scholar

Sievert D. M., M.L. Boulton, G. Stoltman, D. Johnson, M.G. Stobierski, W. Brown, W. Hafeez and T. Lundstrom. 2002. Staphylococcus aureus resistant to vancomycin-United States. MMWR Morb. Mortal. Wkly. Rep. 51: 565–567. Search in Google Scholar

Tavares A.1., C.M. Carvalho, M.A. Faustino, M.G. Neves, J.P. Tomé, A.C. Tomé, J.A. Cavaleiro, A. Cunha, N.C. Gomes, E. Alves and A. Almeida. 2010. Antimicrobial photodynamic therapy: study of bacterial recovery viability and potential development of resistance after treatment. Mar. Drugs. 8: 91–105.10.3390/md8010091 Search in Google Scholar

Wainwright M. and K.B. Crossley. 2004. Photosensitizing agents circumventing resistance and breaking down biofilms: a review. Int. Biodeterior. Biodegrad. 53: 119–126.10.1016/j.ibiod.2003.11.006 Search in Google Scholar

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