<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Pseudomonas aeruginosa</italic> rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections. Antibiotic-resistant <italic>P. aeruginosa</italic> strains may present reduced expression of virulence factors. This fact may be caused by appropriate genome management to adapt to changing conditions of the hospital environment. Virulence factors genes may be replaced by those crucial to survive, like antimicrobial resistance genes. The aim of this study was to evaluate, using PCR, the occurrence of exoenzyme S-coding gene (<italic>exo</italic>S) in two distinct groups of <italic>P. aeruginosa</italic> strains: 83 multidrug-sensitive (MDS) and 65 multidrug-resistant (MDR) isolates. <italic>Exo</italic>S gene was noted in 72 (48.7%) of the examined strains: 44 (53.0%) MDS and 28 (43.1%) MDR. The observed differences were not statistically significant (p = 0.1505). <italic>P. aeruginosa</italic> strains virulence is rather determined by the expression regulation of the possessed genes than the difference in genes frequency amongst strains with different antimicrobial susceptibility patterns.</p>
</abstract>

Pseudomonas aeruginosa rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections. Antibiotic-resistant P. aeruginosa strains may present reduced expression of virulence factors. This fact may be caused by appropriate genome management to adapt to changing conditions of the hospital environment. Virulence factors genes may be replaced by those crucial to survive, like antimicrobial resistance genes. The aim of this study was to evaluate, using PCR, the occurrence of exoenzyme S-coding gene (exoS) in two distinct groups of P. aeruginosa strains: 83 multidrug-sensitive (MDS) and 65 multidrug-resistant (MDR) isolates. ExoS gene was noted in 72 (48.7%) of the examined strains: 44 (53.0%) MDS and 28 (43.1%) MDR. The observed differences were not statistically significant (p = 0.1505). P. aeruginosa strains virulence is rather determined by the expression regulation of the possessed genes than the difference in genes frequency amongst strains with different antimicrobial susceptibility patterns.

The Prevalence of Exoenzyme S Gene in Multidrug-Sensitive and Multidrug-Resistant <italic>Pseudomonas aeruginosa</italic> Clinical Strains

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Prevalence of Exoenzyme S Gene in Multidrug-Sensitive and Multidrug-Resistant  Clinical Strains

Pseudomonas aeruginosa rods are one of the most commonly isolated microorganisms from clinical specimens, usually responsible for nosocomial infections....

The Prevalence of Exoenzyme S Gene in Multidrug-Sensitive and Multidrug-Resistant Pseudomonas aeruginosa Clinical Strains

Published Online: Dec 04, 2017

Page range: 427 - 432

Received: Feb 11, 2017

Accepted: May 17, 2017

DOI: https://doi.org/10.5604/pjm-2017-427

Keywords
exoenzyme S, multidrug sensitive, multidrug resistant, virulence genes

© 2017 TOMASZ BOGIEL et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Prevalence of Exoenzyme S Gene in Multidrug-Sensitive and Multidrug-Resistant Pseudomonas aeruginosa Clinical Strains

Published Online: Dec 04, 2017

Page range: 427 - 432

Received: Feb 11, 2017

Accepted: May 17, 2017

DOI: https://doi.org/10.5604/pjm-2017-427

Keywordsexoenzyme S, multidrug sensitive, multidrug resistant, virulence genes

© 2017 TOMASZ BOGIEL et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
exoenzyme S, multidrug sensitive, multidrug resistant, virulence genes