<abstract xmlns="http://www.w3.org/1999/xhtml">

<italic>Pseudomonas aeruginosa</italic> has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing <italic>P. aeruginosa</italic> in a healthcare setting in Alexandria, Egypt. This cross sectional study included 1583 clinical samples that were collected from patients admitted to Alexandria University Students’ Hospital. <italic>P. aeruginosa</italic> isolates were identified using standard microbiological methods and were tested for their antimicrobial susceptibility patterns using single disc diffusion method according to the Clinical and Laboratory Standards Institute recommendations. Thirty <italic>P. aeruginosa</italic> isolates were randomly selected and tested for their MBL production by both phenotypic and genotypic methods. Diagnostic Epsilometer test was done to detect metallo-beta-lactamase enzyme producers and polymerase chain reaction test was done to detect imipenemase (IMP), Verona integron-encoded (VIM) and Sao Paulo metallo-beta-lactamase (IMP) encoding genes. Of the 1583 clinical samples, 175 (11.3%) <italic>P. aeruginosa</italic> isolates were identified. All the 30 (100%) selected <italic>P. aeruginosa</italic> isolates that were tested for MBL production by Epsilometer test were found to be positive; where 19 (63.3%) revealed <italic>bla</italic>SPM gene and 11 (36.7%) had <italic>bla</italic>IMP gene. <italic>bla</italic>VIM gene was not detected in any of the tested isolates. Isolates of MBL producing <italic>P. aeruginosa</italic> were highly susceptible to polymyxin B 26 (86.7%) and highly resistant to amikacin 26 (86.7%). MBL producers were detected phenotypically by Epsilometer test in both carbapenem susceptible and resistant <italic>P. aeruginosa</italic> isolates. <italic>bla</italic>SPM was the most commonly detected MBL gene in <italic>P. aeruginosa</italic> isolates.
</abstract>

Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. This cross sectional study included 1583 clinical samples that were collected from patients admitted to Alexandria University Students’ Hospital. P. aeruginosa isolates were identified using standard microbiological methods and were tested for their antimicrobial susceptibility patterns using single disc diffusion method according to the Clinical and Laboratory Standards Institute recommendations. Thirty P. aeruginosa isolates were randomly selected and tested for their MBL production by both phenotypic and genotypic methods. Diagnostic Epsilometer test was done to detect metallo-beta-lactamase enzyme producers and polymerase chain reaction test was done to detect imipenemase (IMP), Verona integron-encoded (VIM) and Sao Paulo metallo-beta-lactamase (IMP) encoding genes. Of the 1583 clinical samples, 175 (11.3%) P. aeruginosa isolates were identified. All the 30 (100%) selected P. aeruginosa isolates that were tested for MBL production by Epsilometer test were found to be positive; where 19 (63.3%) revealed blaSPM gene and 11 (36.7%) had blaIMP gene. blaVIM gene was not detected in any of the tested isolates. Isolates of MBL producing P. aeruginosa were highly susceptible to polymyxin B 26 (86.7%) and highly resistant to amikacin 26 (86.7%). MBL producers were detected phenotypically by Epsilometer test in both carbapenem susceptible and resistant P. aeruginosa isolates. blaSPM was the most commonly detected MBL gene in P. aeruginosa isolates.

Metallo-Beta-Lactamase Producing <italic>Pseudomonas aeruginosa</italic> in a Healthcare Setting in Alexandria, Egypt

High Institute of Public Health, Alexandria University

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Metallo-Beta-Lactamase Producing  in a Healthcare Setting in Alexandria, Egypt

Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed...

Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt

Published Online: Sep 27, 2017

Page range: 297 - 308

Received: Dec 07, 2016

Accepted: May 05, 2017

DOI: https://doi.org/10.5604/pjm-2017-297

Keywords
Epsilometer test, metallo-beta-lactamases, MBL encoding genes

© 2017 AMANI F. ABAZA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Metallo-Beta-Lactamase Producing Pseudomonas aeruginosa in a Healthcare Setting in Alexandria, Egypt

Published Online: Sep 27, 2017

Page range: 297 - 308

Received: Dec 07, 2016

Accepted: May 05, 2017

DOI: https://doi.org/10.5604/pjm-2017-297

KeywordsEpsilometer test, metallo-beta-lactamases, MBL encoding genes

© 2017 AMANI F. ABAZA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Epsilometer test, metallo-beta-lactamases, MBL encoding genes