<abstract xmlns="http://www.w3.org/1999/xhtml">

<italic>Ganoderma boninense</italic>, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of <italic>G. boninense</italic> is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 104basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from <italic>Trichoderma harzianum</italic> (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 × 106single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).
</abstract>

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient collection of fruiting body, basidiospore and protoplast of G. boninense is described. Matured basidiocarp raised under the glasshouse conditions yielded a total of 8.3 × 104basidiospores/ml using the low speed centrifugation technique. Mycelium aged 3-day-old treated under an incubation period of 3 h in lysing enzyme from Trichoderma harzianum (10 mg/ml) suspended in osmotic stabilizer (0.6 M potassium chloride and 20 mM dipotassium phosphate buffer) yielded the highest number of viable protoplasts (8.9 × 106single colonies) among all possible combinations tested (regeneration media, age of mycelium, osmotic stabilizer, digestive enzyme and incubation period).

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic <italic>Ganoderma boninense</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic 

Ganoderma boninense, a phytopathogenic white rot fungus had sought minimal genetic characterizations despite huge biotechnological potentials. Thus, efficient...

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense

Published Online: Aug 26, 2016

Page range: 383 - 388

Received: Nov 14, 2015

Accepted: Feb 02, 2016

DOI: https://doi.org/10.5604/17331331.1215619

Keywords
basidiocarp, basidiospore, incubation, lytic enzyme

© 2016 NISHA THOPLA GOVENDER et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Basidiospore and Protoplast Regeneration from Raised Fruiting Bodies of Pathogenic Ganoderma boninense

Published Online: Aug 26, 2016

Page range: 383 - 388

Received: Nov 14, 2015

Accepted: Feb 02, 2016

DOI: https://doi.org/10.5604/17331331.1215619

Keywordsbasidiocarp, basidiospore, incubation, lytic enzyme

© 2016 NISHA THOPLA GOVENDER et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
basidiocarp, basidiospore, incubation, lytic enzyme