<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Fifty seven bacterial isolates from root nodules of two spontaneous legumes (<italic>Astragalus corrugatus</italic> and <italic>Hippocrepis areolata</italic>) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RFLP and 16S rRNA gene sequencing. Phenotypically, our results indicate that <italic>A. corrugatus</italic> and <italic>H. areolata</italic> isolates showed heterogenic responses to the different phenotypic features. All isolates were acid producers, fast growers and all of them used different compounds as sole carbon and nitrogen source. The majority of isolate grew at pHs between 6 and 9, at temperatures up to 40°C and tolerated 3% NaCl concentrations. Phylogenetically, the new isolates were affiliated to four genera <italic>Sinorhizobium</italic>, <italic>Rhizobium, Mesorhizobium</italic> and <italic>Agrobacterium</italic>. About 73% of the isolates were species within the genera <italic>Sinorhizobium</italic> and <italic>Rhizobium</italic>. The isolates which failed to nodulate their host plants of origin were associated to <italic>Agrobacterium</italic> genus (three isolates).</p>
</abstract>

Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of Tunisia were characterized by phenotypic features, 16S rDNA PCR-RFLP and 16S rRNA gene sequencing. Phenotypically, our results indicate that A. corrugatus and H. areolata isolates showed heterogenic responses to the different phenotypic features. All isolates were acid producers, fast growers and all of them used different compounds as sole carbon and nitrogen source. The majority of isolate grew at pHs between 6 and 9, at temperatures up to 40°C and tolerated 3% NaCl concentrations. Phylogenetically, the new isolates were affiliated to four genera Sinorhizobium, Rhizobium, Mesorhizobium and Agrobacterium. About 73% of the isolates were species within the genera Sinorhizobium and Rhizobium. The isolates which failed to nodulate their host plants of origin were associated to Agrobacterium genus (three isolates).

Characterization of Rhizobial Bacteria Nodulating <italic>Astragalus corrugatus</italic> and <italic>Hippocrepis areolata</italic> in Tunisian Arid Soils

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of Rhizobial Bacteria Nodulating  and  in Tunisian Arid Soils

Fifty seven bacterial isolates from root nodules of two spontaneous legumes (Astragalus corrugatus and Hippocrepis areolata) growing in the arid areas of...

Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils

Published Online: Aug 26, 2016

Page range: 331 - 339

Received: Jun 13, 2015

Accepted: Feb 11, 2016

DOI: https://doi.org/10.5604/17331331.1215612

Keywords
16S rDNA sequencing, arid areas, PCR-RFLP, phenotypic properties, rhizobial bacteria

© 2016 MOSBAH MAHDHI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of Rhizobial Bacteria Nodulating Astragalus corrugatus and Hippocrepis areolata in Tunisian Arid Soils

Published Online: Aug 26, 2016

Page range: 331 - 339

Received: Jun 13, 2015

Accepted: Feb 11, 2016

DOI: https://doi.org/10.5604/17331331.1215612

Keywords16S rDNA sequencing, arid areas, PCR-RFLP, phenotypic properties, rhizobial bacteria

© 2016 MOSBAH MAHDHI et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
16S rDNA sequencing, arid areas, PCR-RFLP, phenotypic properties, rhizobial bacteria