<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Pseudomonas aeruginosa</italic> is a leading human pathogen that causes serious infections at various tissues and organs leading to life threatening health problems and possible deadly outcomes. Resistance patterns vary widely whether it is from hospitals or community acquired infections. Reporting resistance profiles to a certain antibiotics provide valuable information in a given setting, but may be extrapolated outside the sampling location. In the present study, <italic>P. aeruginosa</italic> isolates were screened to determine their susceptibilities against anti-pseudomonal antimicrobial agents and possible existing mechanisms of resistance were determined. Eighty-six isolates of <italic>P. aeruginosa</italic> were recovered. Isolates representing different resistance profiles were screened for the existence of three different resistance mechanisms including drug inactivation due to metallo-β-lactamases, drug impermeability by outer membrane proteins and drug efflux. All tested isolates showed uniform susceptibility (100%, n = 86/86) to piperacillin, meropenem, amikacin, and polymyxin B. A single isolate was found to be imipenem resistant (99%, n = 85/86). The possible mechanisms of resistance of <italic>P. aeruginosa</italic> to imipenem involve active drug efflux pumps, outer membrane impermeability as well as drug inactivating enzymes. These findings demonstrate the fundamental importance of the <italic>in vitro</italic> susceptibility testing of antibiotics prior to antipseudomonal therapy and highlight the need for a continuous antimicrobial resistance surveillance programs to monitor the changing resistance patterns so that clinicians and health care officials are updated as to the most effective therapeutic agents to combat the serious outcomes of <italic>P. aeruginosa</italic> infections.</p>
</abstract>

Pseudomonas aeruginosa is a leading human pathogen that causes serious infections at various tissues and organs leading to life threatening health problems and possible deadly outcomes. Resistance patterns vary widely whether it is from hospitals or community acquired infections. Reporting resistance profiles to a certain antibiotics provide valuable information in a given setting, but may be extrapolated outside the sampling location. In the present study, P. aeruginosa isolates were screened to determine their susceptibilities against anti-pseudomonal antimicrobial agents and possible existing mechanisms of resistance were determined. Eighty-six isolates of P. aeruginosa were recovered. Isolates representing different resistance profiles were screened for the existence of three different resistance mechanisms including drug inactivation due to metallo-β-lactamases, drug impermeability by outer membrane proteins and drug efflux. All tested isolates showed uniform susceptibility (100%, n = 86/86) to piperacillin, meropenem, amikacin, and polymyxin B. A single isolate was found to be imipenem resistant (99%, n = 85/86). The possible mechanisms of resistance of P. aeruginosa to imipenem involve active drug efflux pumps, outer membrane impermeability as well as drug inactivating enzymes. These findings demonstrate the fundamental importance of the in vitro susceptibility testing of antibiotics prior to antipseudomonal therapy and highlight the need for a continuous antimicrobial resistance surveillance programs to monitor the changing resistance patterns so that clinicians and health care officials are updated as to the most effective therapeutic agents to combat the serious outcomes of P. aeruginosa infections.

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in <italic>Pseudomonas aeruginosa</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in 

Pseudomonas aeruginosa is a leading human pathogen that causes serious infections at various tissues and organs leading to life threatening health problems and...

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

Published Online: Mar 15, 2016

Page range: 23 - 32

Received: Jan 05, 2015

Accepted: Aug 26, 2015

DOI: https://doi.org/10.5604/17331331.1197272

Keywords
antibiotics, antimicrobial, carbapenems, efflux pump, mechanisms, metallo-β-lactamases, polymyxins, mutidrug, resistance

© 2016 MOHAMED E. EL ZOWALATY et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effectiveness of Antipseudomonal Antibiotics and Mechanisms of Multidrug Resistance in Pseudomonas aeruginosa

Published Online: Mar 15, 2016

Page range: 23 - 32

Received: Jan 05, 2015

Accepted: Aug 26, 2015

DOI: https://doi.org/10.5604/17331331.1197272

Keywordsantibiotics, antimicrobial, carbapenems, efflux pump, mechanisms, metallo-β-lactamases, polymyxins, mutidrug, resistance

© 2016 MOHAMED E. EL ZOWALATY et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
antibiotics, antimicrobial, carbapenems, efflux pump, mechanisms, metallo-β-lactamases, polymyxins, mutidrug, resistance