<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Background</h3><p>Complications of infected wounds in patients with diabetic foot ulcer (DFU) are one of the greatest challenges in modern medicine. Analysis of the microbiological profile of infected ulcers may significantly improve treatment results. The aim of the study was to determine the profile of pathogens isolated in patients with DFU and to compare the results of other centers.</p></sec>
<sec><h3>Materials and Methods</h3><p>A retrospective study was carried out on 137 patients with DFU hospitalized at the Department of Diabetology and Internal Diseases, Medical University of Warsaw in 2011-2014. The analysis included the results of 200 microbiological cultures tested for fungi, aerobic and anaerobic bacteria. Statistical analysis was used to test differences in HbA1c values in relation to the strain of the most commonly cultured bacteria and the relationship between glycemic control and most frequently isolated pathogens.</p></sec>
<sec><h3>Results</h3><p>Seventy-nine bacterial species were isolated in 183 positive cultures. Gram-negative bacteria predominated with the highest percentage of representatives of <italic>Enterobacterales</italic>. The most often isolated bacteria were <italic>Serratia marcescens</italic>, <italic>Pseudomonas aeruginos</italic>a, <italic>Proteus mira-bilis</italic> and methicillin-susceptible <italic>Staphylococcus aureus</italic>. The Kruskal-Wallis test revealed that HbA1c concentrations were different in groups infected with different strains of bacteria (p = 0.0087). Isolation of Escherichia coli and Morganella morganii was more often associated with poor control of diabetes.</p></sec>
<sec><h3>Conclusions</h3><p>The study revealed statistically significant differences in the frequency of microorganisms isolated from the wounds of patients with DFU. The discrepancies in the results of other studies published in this field indicate the need for standardization of the research design.</p></sec>
</abstract>

BackgroundComplications of infected wounds in patients with diabetic foot ulcer (DFU) are one of the greatest challenges in modern medicine. Analysis of the microbiological profile of infected ulcers may significantly improve treatment results. The aim of the study was to determine the profile of pathogens isolated in patients with DFU and to compare the results of other centers.
Materials and MethodsA retrospective study was carried out on 137 patients with DFU hospitalized at the Department of Diabetology and Internal Diseases, Medical University of Warsaw in 2011-2014. The analysis included the results of 200 microbiological cultures tested for fungi, aerobic and anaerobic bacteria. Statistical analysis was used to test differences in HbA1c values in relation to the strain of the most commonly cultured bacteria and the relationship between glycemic control and most frequently isolated pathogens.
ResultsSeventy-nine bacterial species were isolated in 183 positive cultures. Gram-negative bacteria predominated with the highest percentage of representatives of Enterobacterales. The most often isolated bacteria were Serratia marcescens, Pseudomonas aeruginosa, Proteus mira-bilis and methicillin-susceptible Staphylococcus aureus. The Kruskal-Wallis test revealed that HbA1c concentrations were different in groups infected with different strains of bacteria (p = 0.0087). Isolation of Escherichia coli and Morganella morganii was more often associated with poor control of diabetes.
ConclusionsThe study revealed statistically significant differences in the frequency of microorganisms isolated from the wounds of patients with DFU. The discrepancies in the results of other studies published in this field indicate the need for standardization of the research design.

Analysis of the microbiota in the diabetic foot ulcers: Is research standardization required?

Department of Diabetology and Internal Diseases, Central Clinical Hospital

Department of Microbiology, Central Clinical Hospital, University Clinical Centre

Nalecz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences

Department of Internal Medicine, District Hospital in Plonsk

Postępy Higieny i Medycyny Doświadczalnej

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mikrobiologiczna analiza zakażeń w zespole stopy cukrzycowej – czy konieczna jest standaryzacja badań naukowych?

BackgroundComplications of infected wounds in patients with diabetic foot ulcer (DFU) are one of the greatest challenges in modern medicine. Analysis of the...

Analysis of the microbiota in the diabetic foot ulcers: Is research standardization required?

Article Category: Original Article

Published Online: May 25, 2021

Page range: 362 - 370

Received: May 12, 2020

Accepted: Feb 02, 2021

DOI: https://doi.org/10.5604/01.3001.0014.8987

Keywords
diabetic foot ulcer, microbiological culture, HbA1c values, wound infection

© 2021 Marta Margas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Analysis of the microbiota in the diabetic foot ulcers: Is research standardization required?

Article Category: Original Article

Published Online: May 25, 2021

Page range: 362 - 370

Received: May 12, 2020

Accepted: Feb 02, 2021

DOI: https://doi.org/10.5604/01.3001.0014.8987

Keywordsdiabetic foot ulcer, microbiological culture, HbA1c values, wound infection

© 2021 Marta Margas et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
diabetic foot ulcer, microbiological culture, HbA1c values, wound infection