<abstract xmlns="http://www.w3.org/1999/xhtml"><p>We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in <italic>Bacteroides</italic> spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and <italic>cfiA</italic> gene was investigated by polymerase chain reaction for 27 clinical <italic>Bacteroides</italic> spp. isolates. Carba NP test was performed according to recommendations of the Clinical and Laboratory Standards Institute. Among three <italic>cfiA</italic> gene harboring clinical isolates, two imipenem resistant isolates were Carba NP test positive, while the imipenem intermediate isolate was negative. Our preliminary results suggest that the Carba NP test can be useful as a rapid test to detect carbapenemases in <italic>Bacteroides</italic> species.</p></abstract>

We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and cfiA gene was investigated by polymerase chain reaction for 27 clinical Bacteroides spp. isolates. Carba NP test was performed according to recommendations of the Clinical and Laboratory Standards Institute. Among three cfiA gene harboring clinical isolates, two imipenem resistant isolates were Carba NP test positive, while the imipenem intermediate isolate was negative. Our preliminary results suggest that the Carba NP test can be useful as a rapid test to detect carbapenemases in Bacteroides species.


<div xmlns="http://www.w3.org/1999/xhtml"><p><italic>Bacteroides fragilis</italic> is one of the most important causes of intraabdominal infections and bacteremia which might be fatal if not properly treated (Wexler <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_017">2007</a>). Carbapenems are generally used for the treatment of anaerobic infections, but production of carbapenemases by <italic>Bacteroides</italic> spp. renders these antimicrobials ineffective (Sóki <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_013">2000</a>). An important mechanism of carbapenem resistance in <italic>Bacteroides</italic> spp. is the acquisition of the <italic>cfiA</italic> gene encoding a metallo-beta-lactamase enzyme that is able to hydrolyse carbapenemes. If present, the <italic>cfiA</italic> gene may or may not cause clinical resistance depending on expression level of enzyme, or else the gene may stay completely silent (Ang <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_001">2007</a>). The molecular detection of the <italic>cfiA</italic> gene by itself is not indicative of clinical carbapenem resistance; supplementary phenotypic tests are needed to determine the carbapenem susceptibility of clinical <italic>Bacteroides</italic> isolates, such as performance of the minimal inhibitory concentration (MIC) testing for carbapenems.</p><p>Laboratory detection of the carbapenemase genes is challenging, moreover carbapenemase-producing bacteria are spreading throughout the world (Cantón <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_002">2012</a>). Many strains have carbapenem MICs in the susceptible range, and different phenotypic methods designed to detect such strains lack specificity and sensitivity such as the modified Hodge test, and combined disk tests with different inhibitors (Nordmann <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_009">2012</a>). Detection of carbapenemase genes and demonstration of the ability of a strain to hydrolyze carbapenems still remain the gold standard methods for identification of carbapenemase-producing isolates (Poirel <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_011">2013</a>). The recently published Carba NP test is described as a biochemical method based on imipenem hydrolysis (Nordmann <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_009">2012</a>). Without any specialized equipment, the CARBA NP test detects the pH change by colorimetric pH indicator caused by the breakdown of imipenem in a solution containing lysed test bacteria. The test’s specificity and sensitivity were initially reported as 100% when results were compared with those of molecular-based methods, the reference standard for identifying carbapenemase genes (Nordmann <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_009">2012</a>).</p><p>The usefulness of Carba NP test has been demonstrated for many Gram-negative bacteria, including <italic>Enterobacteriacae, Acinetobacter</italic> spp. and <italic>Pseudomonas aeruginosa</italic> (Nordmann <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_009">2012</a>; Dortet <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_003">2012</a>; Dortet <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_004">2014</a>). In this study, our aim was to investigate the carbapenemase activity in clinical <italic>Bacteroides</italic> spp. isolates by Carba NP test and compare the results obtained from the Carba NP test with phenotypic (carbapenem MICs) and genotypic (presence or absence of the <italic>cfiA</italic> gene), and thus assess the usefulness of the Carba NP test for the detection of carbapenemase activity in <italic>Bacterodies</italic> spp.</p><p>A total of 27 clinical <italic>Bacteroides</italic> spp. isolates collected between 2011 and 2016 were included in this study. The distribution of strains to isolation sites is presented in Table <a ref-type="table" href="#j_01.3001.0011.6148_tab_001">I</a>. All isolates were stored at −80°C in cryopreservation vials (Salubris, Turkey). Frozen stock cultures were used to inoculate subcultures which were routinely grown on Schaedler agar (bioMérieux, France) and incubated at 35–37°C in anaerobe pouch system (GasPak<sup>™</sup> EZ Anaerobe Pouch System, Becton Dickinson, USA). For species identification matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (Bruker Daltonics, Germany) was used.</p><table-wrap id="j_01.3001.0011.6148_tab_001" position="float"><label>Table I</label><caption><p>Distribution of the clinical Bacteroides spp. isolates and sources.</p></caption></table-wrap><p>The susceptibility of the <italic>Bacteroides</italic> spp. to imipenem (IMP) was determined by gradient strips according to the manufacturer’s recommendations. In the current study, bacterial suspension matched to 1 McFarland standard was prepared from fresh colonies and inoculated onto Brucella agar with 5% sheep blood supplemented with hemin and vitamin K1 (Salubris, Turkey), then an IMP gradient strip was placed onto agar surface for each isolate (Oxoid<sup>™</sup> M.I.C.Evaluator<sup>™</sup> Strips, Thermo Scientific, UK). The plates were incubated in anaerobe pouch system at 35–37°C for 24–48 hours. <italic>B. fragilis</italic> ATCC 25285 was used as a reference strain. The clinical breakpoints recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) were used to interpret the results (EUCAST, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_007">2016</a>).</p><p>Bacterial DNA was extracted by a commercial kit (High Pure PCR Template Preparation Kit, Roche, Germany). The carbapenemase coding gene <italic>cfiA</italic> was amplified by polymerase chain reaction (PCR) with the following primers: cfiA-RT1 (5’-AATCGAAGGA TGGG GTATGG-3’) and cfiA-RT2 (5’-CGGTCGGTG AATCGGTGAAT-3’) which amplify 300 bp of the 750 bp <italic>cfiA</italic> gene (Sóki <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_012">2013</a>). A <italic>B. fragilis</italic> strain with previously documented <italic>cfi</italic>A positivity was used as a positive control in PCR studies (Toprak <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_015">2012</a>).The PCR cycles were as follows: 2 minutes at 94°C, 35 cycles (45 seconds at 94°C, 45 seconds at 51°C, 45 seconds at 72°C) and 2 minutes at 72°C. For the visualisation of PCR products, 2% agarose gel was used which were stained with SYBR gold and monitored using ORTE device (Salubris Technica, Istanbul, Turkey).</p><p>Carba NP test was performed according to CLSI recommendations (CLSI, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_016">2016</a>). For each strain two microcentrifuge tubes (1.5 ml) labeled as “a” and “b” were used. The bacterial colonies grown on Schaedler agar were collected after 24 hours and a loopful of bacteria (approximately 10 µl) were added into 100 μl of bacterial protein extraction reagent (B-PERII, Thermo Scientific, Pierce) and stirred for 5 seconds. From the solutions A (containing zinc sulphate and phenol red, pH: 7.8 ± 0.1) and B (containing solution A and 6 mg/ml imipenem), 100 μl solution was added to a, and b tubes, respectively, and then incubated at 35 ± 2°C for up to two hours. The results were interpreted according to the color changes of the tubes. The carbapenemase-producing <italic>B. fragilis</italic> strain TAL 2480 was used as positive control and <italic>B. fragilis</italic> ATCC 25285 was used as negative control. The microcentrifuge tubes which contain only bacterial protein extraction reagents were prepared as reagent control. When imipenem was hydrolysed, the color of the tube’s content turned from red to orange or yellow which was interpreted as a positive Carba NP test, whereas tubes containing bacterial extracts of isolates with no carbapenemase activity remained red. The color changed from red to yellow as early as 30 minutes after incubation with <italic>B. fragilis</italic> TAL 2480 (Fig. <a ref-type="fig" href="#j_01.3001.0011.6148_fig_001">1</a>).</p><figure id="j_01.3001.0011.6148_fig_001" fig-type="figure"><h2>Fig. 1.</h2><figCaption><p>Interpretation of the Carba NP test results: <italic>B. fragilis</italic> ATCC 25285 with no carbapenemase activity (left) and carbapenemase-producing <italic>B. fragilis</italic> strain TAL 2480 (right).</p><p>The tubes denoted as “a” contain solution A + bacterial lysate, tubes “b” contain solution B (solution A + imipenem) + bacterial lysate. If acidification occurs as a result of hydrolysis of imipenem, the pH indicator (phenol red) causes a color change from red to yellow in tube b.</p></figCaption><img xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_01.3001.0011.6148_fig_001.jpg" src="https://sciendo-parsed.s3.eu-central-1.amazonaws.com/64727a7d215d2f6c89dc9cc7/j_01.3001.0011.6148_fig_001.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&amp;X-Amz-Date=20240520T183028Z&amp;X-Amz-SignedHeaders=host&amp;X-Amz-Expires=18000&amp;X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20240520%2Feu-central-1%2Fs3%2Faws4_request&amp;X-Amz-Signature=11fc6da702b97c3d13156a0cdffe27e3aff200e8ec00577dfc134101598dbd75" class="mw-100"/></figure><p>Imipenem MIC values determined by gradient strips ranged from 0.03 to ≥ 32 mg/l among study isolates. Majority (24 out of 27; 88.9%) of the strains were found susceptible to imipenem (MIC ≤ 2 mg/l) with EUCAST breakpoints, one strain showed intermediate susceptibility (MIC = 4 mg/l) and two of the strains were found resistant to imipenem (MIC ≥ 16 mg/l). The <italic>cfi</italic>A gene was detected in three isolates (11.1%), all <italic>B. fragilis</italic> blood isolates with imipenem MIC ≥ 4 μg/ml. A correlation between imipenem MIC being ≥ 4 μg/ml and <italic>cfi</italic>A gene positivity was observed for the tested <italic>Bacteroides</italic> sp. isolates. Carba NP test results were obtained in 2 hours in clinical isolates and in 30 minutes for the reference strain. The two clinical strains with imipenem MIC ≥ 32 mg/l and the reference <italic>cfi</italic>A positive strain were tested positive in the Carba NP test, however the imipenem intermediate (MIC = 4 mg/l), <italic>cfi</italic>A positive strain was found negative (Table <a ref-type="table" href="#j_01.3001.0011.6148_tab_002">II</a>).</p><table-wrap id="j_01.3001.0011.6148_tab_002" position="float"><label>Table II</label><caption><p>Correlation of <italic>cfiA</italic> gene presence and Carba NP test positivity with imipenem MICs in clinical <italic>Bacteroides</italic> spp. isolates (n = 27).</p></caption></table-wrap><figure id="j_01.3001.0011.6148_fig_002" fig-type="figure"><h2>Fig. 2.</h2><figCaption><p>The PCR amplification results for the <italic>cfiA</italic> gene in clinical <italic>Bacteroides</italic> spp. isolates.</p><p>Lanes 1, 3 and 4 are clinical <italic>B. fragilis</italic> isolates demonstrating the presence of the <italic>cfiA</italic>X gene, lanes 2, 5–10 are clinical <italic>Bacteroides</italic> spp. lacking the <italic>cfia</italic> gene, lane 11 is negative control (water), lane 12 is a <italic>cfiA</italic> positive <italic>B. fragilis</italic> strain used as positive control. Molecular sizes in base pair (bp) are indicated at the left. (M: Molecular mass standard) This PCR assay was used to amplify a 302 bp fragment of the <italic>cfiA</italic> gene.</p></figCaption><img xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_01.3001.0011.6148_fig_002.jpg" src="https://sciendo-parsed.s3.eu-central-1.amazonaws.com/64727a7d215d2f6c89dc9cc7/j_01.3001.0011.6148_fig_002.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&amp;X-Amz-Date=20240520T183028Z&amp;X-Amz-SignedHeaders=host&amp;X-Amz-Expires=18000&amp;X-Amz-Credential=AKIA6AP2G7AKP25APDM2%2F20240520%2Feu-central-1%2Fs3%2Faws4_request&amp;X-Amz-Signature=72d72d703373f8854baa2bacebdc3c1abf789c8b1722276ba0040cdc6fb30c06" class="mw-100"/></figure><p>In the present study, we aimed to evaluate the applicability of the Carba NP test, which was originally developed for <italic>Enterobacteriaceae</italic>, to investigate the <italic>cfiA</italic>-mediated carbapenemase production in <italic>Bacteroides</italic> spp. The <italic>cfiA</italic> gene was investigated in a collection of clinical <italic>Bacteroides</italic> sp. isolates (n = 27) and we found three (11.1%) <italic>cfiA</italic> positive <italic>B. fragilis</italic> isolates. The Carba NP test yielded positive results for two of the <italic>cfiA</italic> positive isolates both having imipenem MICs of ≥ 16 mg/l but the test gave negative results for the <italic>cfiA</italic> positive isolate with imipenem MIC of 4 mg/l. No positive Carba NP test results were obtained in imipenem susceptible (MIC ≤ 2 mg/l) and <italic>cfiA</italic> negative isolates. Our results with the limited isolate collection suggest that Carba NP test can also be used for <italic>Bacteroides</italic> spp. to have preliminary information on carbapenem susceptibility. The positive results with the Carba NP test are very likely to be related with high carbapenem MIC values which might be taken as a warning for treatment failure if carbapenems are used.</p><p><italic>Bacteroides</italic> spp. are the predominant members of the human intestinal microbiota and constitute approximately 30% of the total colonic bacteria. They are also among the most commonly isolated anaerobic bacteria from clinical specimens. The <italic>B. fragilis</italic> group is of special medical importance since they are often involved in polymicrobial infections, bear the potential to express β-lactamases and are associated with a high mortality when causing bloodstream infections (Wexler <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_017">2007</a>). A French multicenter study determined the role of anaerobe bacteria in the aetiology of all bacteremias as 0.5% to 9%, of which 60% being caused by <italic>Bacteroides</italic> spp. (Zahar <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_018">2005</a>). These factors emphasize the importance of identification and rapid antimicrobial susceptibility testing for clinical <italic>Bacteroides</italic> spp. isolates for the administration of appropriate antibiotic therapy to achieve optimal outcomes. Access to rapid susceptibility results is important in the case of infections due to <italic>Bacteroides</italic> spp. since the susceptibility of these bacteria cannot be predicted due to the ability of the organism to acquire resistance mechanisms. The <italic>B. fragilis</italic> group are uniformly resistant to penicillin and first generation cephalosporins which leaves limited number of alternatives such as metronidazole, imipenem, piperacillin-tazobactam, moxifloxacin and clindamycin with reported resistance rates of &lt; 1%, 1.2%, 3.1%, 13.6%, and 32.4%, respectively (Nagy <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_008">2011</a>). A Europe-wide study showed that imipenem retains its activity against <italic>B. fragilis</italic> isolates, but the percentage of isolates with reduced susceptibility (MIC: ≥ 4 mg/L) are steadily increasing (Nagy <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_008">2011</a>). Carbapenem resistance in <italic>B. fragilis</italic> is most commonly related to the acquisition of <italic>cfiA</italic> gene which – if expressed at high levels due to acquisition of an insertion sequence upstream of the gene – can lead to high-level carbapenem resistance (Thompson <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_014">1990</a>; Edwards <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_005">2000</a>). The frequency of <italic>cfiA</italic> positivity in <italic>Bacteroides</italic> spp. has been reported to be as high as 27% in a Turkish study (Toprak <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_015">2012</a>), raising concern as an emerging resistance trait that may limit the empirical use of carbapenems in infections due to <italic>Bacteroides</italic> spp.</p><p>Our study findings revealed the rate of <italic>cfiA</italic> positivity as 11.1% in a small collection of clinical <italic>Bacteroides</italic> sp. isolates. This is in concordance with previously published literature in which rates ranging between 0 and 13% was observed for a collection of 161 <italic>B. fragilis</italic> group strains isolated from nine different European countries (Eitel <italic>et al.</italic>, <a ref-type="bibr" href="#j_01.3001.0011.6148_ref_006">2013</a>). The high rate of 27% <italic>cfiA</italic> positivity reported from Istanbul, Turkey in this study, might be related with the institutional spread of a resistant clone.</p><p>We also had the opportunity to test other <italic>Bacteriodes</italic> species by including a few <italic>B. thetaiotaomicron, B. caccae</italic> and <italic>B. vulgatus</italic> isolates, however the study collection mostly consisted of clinical <italic>B. fragilis</italic> isolates. The total number of isolates tested is relatively low for a validation study, but the collection included <italic>cfiA</italic> gene positive and negative isolates with varying imipenem MIC values that enabled us to investigate the performance of the test. Additionally, the high concordance observed between the phenotypic and genotypic test results further supports the applicability of CARBA NP test in <italic>Bacteroides</italic> spp.</p><p>In our study, we demonstrated the usefulness of the recently described Carba NP test to detect carbapenemase activity in clinical <italic>Bacteroides</italic> spp. isolates. Although further studies are needed, with our preliminary results, we can suggest that the Carba NP test holds the potential to be used as a rapid phenotypic carbapenemase detection test for <italic>Bacteroides</italic> species. The rapid detection of carbapenemase activity in <italic>Bacteroides</italic> spp. might provide early insight into susceptibility of the organism before the final results of the conventional antimicrobial susceptibility are obtained, and thus serve as an important tool to manage antimicrobial therapy.</p></div>

Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in <italic>Bacteroides</italic> Species

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in  Species

We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC)...

Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species

Article Category: short-communication

Published Online: Feb 21, 2022

Page range: 97 - 101

Received: Oct 19, 2016

Accepted: Dec 28, 2017

DOI: https://doi.org/10.5604/01.3001.0011.6148

Keywords
carbapenemase, Carba NP, imipenem

© 2021 Isin Akyar et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Evaluation of the Carba NP Test for the Detection of Carbapenemase Activity in Bacteroides Species

Article Category: short-communication

Published Online: Feb 21, 2022

Page range: 97 - 101

Received: Oct 19, 2016

Accepted: Dec 28, 2017

DOI: https://doi.org/10.5604/01.3001.0011.6148

Keywordscarbapenemase, Carba NP, imipenem

© 2021 Isin Akyar et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
carbapenemase, Carba NP, imipenem