<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently received attention for its potential biotechnological applications. However, information on the enrichment and production of ureolytic microbes by using bioreactor systems is limited. Here, we report a low-tech down-flow hanging sponge (DHS) bioreactor system for the enrichment and production of ureolytic microbes. Using this bioreactor system and a yeast extract-based medium containing 0.17 M urea, ureolytic microbes with high potential urease activity (> 10 μmol urea hydrolyzed per min per ml of enrichment culture) were repeatedly enriched under non-sterile conditions. In addition, the ureolytic enrichment obtained in this study showed <italic>in vitro</italic> calcium carbonate precipitation. Fluorescence <italic>in situ</italic> hybridization analysis showed the existence of bacteria of the phylum <italic>Firmicutes</italic> in the bioreactor system. Our data demonstrate that this DHS bioreactor system is a useful system for the enrichment and production of ureolytic microbes for MICP applications.</p></abstract>

Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently received attention for its potential biotechnological applications. However, information on the enrichment and production of ureolytic microbes by using bioreactor systems is limited. Here, we report a low-tech down-flow hanging sponge (DHS) bioreactor system for the enrichment and production of ureolytic microbes. Using this bioreactor system and a yeast extract-based medium containing 0.17 M urea, ureolytic microbes with high potential urease activity (> 10 μmol urea hydrolyzed per min per ml of enrichment culture) were repeatedly enriched under non-sterile conditions. In addition, the ureolytic enrichment obtained in this study showed in vitro calcium carbonate precipitation. Fluorescence in situ hybridization analysis showed the existence of bacteria of the phylum Firmicutes in the bioreactor system. Our data demonstrate that this DHS bioreactor system is a useful system for the enrichment and production of ureolytic microbes for MICP applications.

A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Ureolysis-driven microbially induced carbonate precipitation (MICP) has recently received attention for its potential biotechnological applications. However,...

A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes

Article Category: original-paper

Published Online: Feb 21, 2022

Page range: 59 - 65

Received: May 16, 2017

Accepted: Sep 27, 2017

DOI: https://doi.org/10.5604/01.3001.0011.6144

Keywords
DHS bioreactor system, enrichment culture, microbially induced carbonate precipitation (MICP), ureolytic microbes

© 2021 Masataka Aoki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

A Low-Tech Bioreactor System for the Enrichment and Production of Ureolytic Microbes

Article Category: original-paper

Published Online: Feb 21, 2022

Page range: 59 - 65

Received: May 16, 2017

Accepted: Sep 27, 2017

DOI: https://doi.org/10.5604/01.3001.0011.6144

KeywordsDHS bioreactor system, enrichment culture, microbially induced carbonate precipitation (MICP), ureolytic microbes

© 2021 Masataka Aoki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
DHS bioreactor system, enrichment culture, microbially induced carbonate precipitation (MICP), ureolytic microbes