<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Pseudomonas putida</italic> strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the <italic>P. putida</italic> strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. Furthermore, the toluene di-oxygenase (<italic>tod</italic>) gene was up-regulated in <italic>P. putida</italic> strain when exposed to toluene, ethylbenzene, xylene, and BTEX. In contrast, the <italic>tod</italic> gene of <italic>P. putida</italic> F1 (ATCC 700007) was up-regulated only in the presence of toluene and BTEX. Several differences in the nucleotide and protein sequences of these two <italic>tod</italic> genes were observed. This suggests that <italic>tod</italic> up-regulation in <italic>P. putida</italic> strain may partially explain their great capacity to remove aromatic compounds, relative to <italic>P. putida</italic> F1. Therefore, new <italic>tod</italic> and <italic>P. putida</italic> strain are promising for various environmental applications.</p>
</abstract>

Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (BTEX) isomers, and a similar capability was observed with the P. putida strain. Proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. Furthermore, the toluene di-oxygenase (tod) gene was up-regulated in P. putida strain when exposed to toluene, ethylbenzene, xylene, and BTEX. In contrast, the tod gene of P. putida F1 (ATCC 700007) was up-regulated only in the presence of toluene and BTEX. Several differences in the nucleotide and protein sequences of these two tod genes were observed. This suggests that tod up-regulation in P. putida strain may partially explain their great capacity to remove aromatic compounds, relative to P. putida F1. Therefore, new tod and P. putida strain are promising for various environmental applications.

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a <italic>Pseudomonas putida</italic> Strain (HM346961) Isolated from It

Department of Biochemistry and Molecular Medicine

Department of Microbiology, Faculty of Medicine

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a  Strain (HM346961) Isolated from It

Pseudomonas putida strain (HM346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. The consortium can...

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

Published Online: Jun 28, 2017

Page range: 189 - 199

Received: Sep 21, 2016

Accepted: Dec 06, 2016

DOI: https://doi.org/10.5604/01.3001.0010.7836

Keywords
spp. BTEX, dioxygenases, LC/MS/MS, bioremediation, biodegradation

© 2017 JESÚS A. MORLETT CHÁVEZ et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Gene Expression during BTEX Biodegradation by a Microbial Consortium Acclimatized to Unleaded Gasoline and a Pseudomonas putida Strain (HM346961) Isolated from It

Published Online: Jun 28, 2017

Page range: 189 - 199

Received: Sep 21, 2016

Accepted: Dec 06, 2016

DOI: https://doi.org/10.5604/01.3001.0010.7836

Keywordsspp. BTEX, dioxygenases, LC/MS/MS, bioremediation, biodegradation

© 2017 JESÚS A. MORLETT CHÁVEZ et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
spp. BTEX, dioxygenases, LC/MS/MS, bioremediation, biodegradation