<abstract xmlns="http://www.w3.org/1999/xhtml">

Growth kinetics of four <italic>Trichoderma</italic> strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of <italic>Trichoderma</italic> biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media. Although the process of lyophilization reduced the survival rate by 50–60%, the actual number of viable cells in obtained biopreparations remained relatively high (0.58 × 108 – 1.68 × 108 CFU/g dm). The studied strains in the preserved biopreparations were characterized by a high growth rate, as evaluated in microcultures using the Bioscreen C system.
</abstract>

Growth kinetics of four Trichoderma strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media. Although the process of lyophilization reduced the survival rate by 50–60%, the actual number of viable cells in obtained biopreparations remained relatively high (0.58 × 108 – 1.68 × 108 CFU/g dm). The studied strains in the preserved biopreparations were characterized by a high growth rate, as evaluated in microcultures using the Bioscreen C system.

Effect of Lyophilization on Survivability and Growth Kinetic of <italic>Trichoderma</italic> Strains Preserved on Various Agriculture By-Products

Wroclaw University of Environmental and Life Sciences

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effect of Lyophilization on Survivability and Growth Kinetic of  Strains Preserved on Various Agriculture By-Products

Growth kinetics of four Trichoderma strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for...

Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products

Published Online: Jun 28, 2017

Page range: 181 - 188

Received: Aug 26, 2016

Accepted: Nov 21, 2016

DOI: https://doi.org/10.5604/01.3001.0010.7835

Keywords
sp., Bioscreen C system, lignocellulosic carriers, lsurvivability after lyophilization

© 2017 DANUTA WITKOWSKA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products

Published Online: Jun 28, 2017

Page range: 181 - 188

Received: Aug 26, 2016

Accepted: Nov 21, 2016

DOI: https://doi.org/10.5604/01.3001.0010.7835

Keywordssp., Bioscreen C system, lignocellulosic carriers, lsurvivability after lyophilization

© 2017 DANUTA WITKOWSKA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
sp., Bioscreen C system, lignocellulosic carriers, lsurvivability after lyophilization