<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The aim of this study was to explore the plasmid characteristics of eight clinical <italic>Enterobacteriaceae</italic> strains containing extended broad spectrum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resistance determinants were investigated by PCR. We showed that at least one plasmid harbouring <italic>qnr</italic>B or <italic>qnr</italic>S determinant was transferred by conjugation in five isolates. <italic>Qep</italic>A determinant was confirmed to be on a non-conjugative plasmid. We found at least one beta-lactamase gene in seven of the eight clinical isolates having plasmid-mediated quinolone resistance, which indicated that these two resistance determinants were mostly on the same conjugative plasmids.</p>
</abstract>

The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spectrum beta-lactamases and plasmid-mediated quinolone resistance. Plasmids were transferred by conjugation or transformation and resistance determinants were investigated by PCR. We showed that at least one plasmid harbouring qnrB or qnrS determinant was transferred by conjugation in five isolates. QepA determinant was confirmed to be on a non-conjugative plasmid. We found at least one beta-lactamase gene in seven of the eight clinical isolates having plasmid-mediated quinolone resistance, which indicated that these two resistance determinants were mostly on the same conjugative plasmids.

Analyses of Plasmids Harbouring Quinolone Resistance Determinants in <italic>Enterobacteriaceae</italic> Members

Department of Pharmaceutical Microbiology, Faculty of Pharmacy

Department of Medical Microbiology, Faculty of Medicine

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Analyses of Plasmids Harbouring Quinolone Resistance Determinants in  Members

The aim of this study was to explore the plasmid characteristics of eight clinical Enterobacteriaceae strains containing extended broad spectrum...

Analyses of Plasmids Harbouring Quinolone Resistance Determinants in Enterobacteriaceae Members

Published Online: Dec 04, 2017

Page range: 529 - 532

Received: Dec 22, 2017

Accepted: Apr 04, 2017

DOI: https://doi.org/10.5604/01.3001.0010.7084

Keywords
beta-lactamase, ESBL-producing, plasmid-mediated quinolone resistance

© 2017 BAYRI ERAC et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Analyses of Plasmids Harbouring Quinolone Resistance Determinants in Enterobacteriaceae Members

Published Online: Dec 04, 2017

Page range: 529 - 532

Received: Dec 22, 2017

Accepted: Apr 04, 2017

DOI: https://doi.org/10.5604/01.3001.0010.7084

Keywordsbeta-lactamase, ESBL-producing, plasmid-mediated quinolone resistance

© 2017 BAYRI ERAC et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
beta-lactamase, ESBL-producing, plasmid-mediated quinolone resistance