<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investigate the microbiomes associated with belowground (rhizosphere), internal (endosphere) and aboveground (phyllosphere) tissues of halophyte (<italic>Salsola stocksii</italic>) through metagenomics approach. Plant samples were collected from Khewra Salt Mines. The metagenomic DNA from soil, root and shoot samples was isolated with the help of FastDNA spin kit. Through PCR, the 16S rRNA gene from four different <italic>Salsola</italic> plants and wheat plants was amplified and cloned in InsTAclone PCR cloning kit. Metagenomic analyses from rhizosphere, endosphere and phyllosphere of <italic>Salsola</italic> showed that approximately 29% bacteria were uncultured and unclassified. <italic>Proteobacteria</italic> and <italic>Actinobacteria</italic> were the most abundant phyla in <italic>Salsola</italic> and wheat. However, <italic>Firmicutes</italic>, <italic>Acidobacteria</italic>, <italic>Bacteriodetes</italic>, <italic>Planctomycetes</italic>, <italic>Cyanobacteria</italic>, <italic>Thermotogae</italic>, <italic>Verrucomicrobia</italic>, <italic>Choroflexi</italic> and <italic>Euryarchaeota</italic> were predominant groups from halophyte whereas <italic>Actinobacteria</italic>, <italic>Proteobacteria</italic>, <italic>Firmicutes</italic>, <italic>Cyanobacteria</italic>, <italic>Acidobacteria</italic>, <italic>Bacteriodetes</italic>, <italic>Planctomycetes</italic> and <italic>Verrucomicrobia</italic> were predominant phyla of wheat samples. Diversity and differences of microbial flora of <italic>Salsola</italic> and wheat suggested that functional interactions between plants and microorganisms contribute to salt stress tolerance.</p>
</abstract>

Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in osmoregulation of these plants. The objective of this study is to investigate the microbiomes associated with belowground (rhizosphere), internal (endosphere) and aboveground (phyllosphere) tissues of halophyte (Salsola stocksii) through metagenomics approach. Plant samples were collected from Khewra Salt Mines. The metagenomic DNA from soil, root and shoot samples was isolated with the help of FastDNA spin kit. Through PCR, the 16S rRNA gene from four different Salsola plants and wheat plants was amplified and cloned in InsTAclone PCR cloning kit. Metagenomic analyses from rhizosphere, endosphere and phyllosphere of Salsola showed that approximately 29% bacteria were uncultured and unclassified. Proteobacteria and Actinobacteria were the most abundant phyla in Salsola and wheat. However, Firmicutes, Acidobacteria, Bacteriodetes, Planctomycetes, Cyanobacteria, Thermotogae, Verrucomicrobia, Choroflexi and Euryarchaeota were predominant groups from halophyte whereas Actinobacteria, Proteobacteria, Firmicutes, Cyanobacteria, Acidobacteria, Bacteriodetes, Planctomycetes and Verrucomicrobia were predominant phyla of wheat samples. Diversity and differences of microbial flora of Salsola and wheat suggested that functional interactions between plants and microorganisms contribute to salt stress tolerance.

Comparison of Microbial Communities Associated with Halophyte (<italic>Salsola stocksii</italic>) and Non-Halophyte (<italic>Triticum aestivum</italic>) Using Culture-Independent Approaches

Forman Christian College (A Chartered University)

National Institute for Biotechnology and Genetic Engineering (NIBGE)

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Comparison of Microbial Communities Associated with Halophyte () and Non-Halophyte () Using Culture-Independent Approaches

Halophyte microbiome contributes significantly to plant performance and can provide information regarding complex ecological processes involved in...

Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approaches

Published Online: Sep 27, 2017

Page range: 353 - 364

Received: Nov 10, 2016

Accepted: Jan 12, 2017

DOI: https://doi.org/10.5604/01.3001.0010.4866

Keywords
16S rRNA gene approach, microbial communities associated with plants, microbiome of halophyte

© 2017 SALMA MUKHTAR et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Comparison of Microbial Communities Associated with Halophyte (Salsola stocksii) and Non-Halophyte (Triticum aestivum) Using Culture-Independent Approaches

Published Online: Sep 27, 2017

Page range: 353 - 364

Received: Nov 10, 2016

Accepted: Jan 12, 2017

DOI: https://doi.org/10.5604/01.3001.0010.4866

Keywords16S rRNA gene approach, microbial communities associated with plants, microbiome of halophyte

© 2017 SALMA MUKHTAR et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
16S rRNA gene approach, microbial communities associated with plants, microbiome of halophyte