<abstract xmlns="http://www.w3.org/1999/xhtml">

<p><italic>Delftia acidovorans</italic> MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml<sup>–1</sup> substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.</p>
</abstract>

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of 9α-hydroxylase inhibitors was not required for preventing the accumulation of byproducts. The effect of pH, temperature, substrate concentration, surfactants and carrier solvents on this bioconversion has been studied. 16-DPA was maximally converted in buffered medium at pH 7.0, at temperature 30°C and 0.5 mg ml–1 substrate concentration. Detergent addition and temperature above 35°C had deleterious effect on bioconversion. Dioxan was found to be the best carrier solvent for biotransformation of 16-DPA to AD.

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by <italic>Delftia acidovorans</italic> MTCC 3363

School of Life Sciences, Devi Ahilya University

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by  MTCC 3363

Delftia acidovorans MTCC 3363 was found to convert 16-dehydropregnenolone acetate (16-DPA) exclusively to 4-androstene-3, 17-dione (AD). Addition of...

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Published Online: Sep 27, 2017

Page range: 321 - 326

Received: Aug 09, 2014

Accepted: Feb 18, 2017

DOI: https://doi.org/10.5604/01.3001.0010.4858

Keywords
MTCC 3363, 4-androstene-3, 17-dione, 16-dehydropregnenolone acetate and steroid bioconversion

© 2017 PUSHPENDRA AWADHIYA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bioconversion of 16-dehydropregnenolone Acetate to Exclusively 4-androstene-3,17-dione by Delftia acidovorans MTCC 3363

Published Online: Sep 27, 2017

Page range: 321 - 326

Received: Aug 09, 2014

Accepted: Feb 18, 2017

DOI: https://doi.org/10.5604/01.3001.0010.4858

KeywordsMTCC 3363, 4-androstene-3, 17-dione, 16-dehydropregnenolone acetate and steroid bioconversion

© 2017 PUSHPENDRA AWADHIYA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
MTCC 3363, 4-androstene-3, 17-dione, 16-dehydropregnenolone acetate and steroid bioconversion