<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_1905-7415.1001.464_s_001">
<h3>Background</h3>
<p>Enterococci comprise a small share of oral flora and occur as contaminants in food such as meat and cheese. They are commonly encountered in patients with infections of oral tissues such as necrotic pulp, root canals and periodontitis.</p>
</sec>
<sec id="j_1905-7415.1001.464_s_002">
<h3>Objective</h3>
<p>To characterize the genotypes of Enterococcus isolated from patients with oral infection and to determine their antibiotic susceptibility.</p>
</sec>
<sec id="j_1905-7415.1001.464_s_003">
<h3>Method</h3>
<p>We genotypically characterized a number of isolates of Enterococcus species derived from patients with various oral infections. All the isolates were tested for the presence of putative virulence genes; efaA (gene for endocarditis), gelE (gene for gelatinase), ace (gene for collagen binding antigen), asa (gene for aggregation substance), cylA (gene for cytolysin activator), and esp (gene for surface adhesin) of E. faecalis and E. faecium as described in previous studies.</p>
</sec>
<sec id="j_1905-7415.1001.464_s_004">
<h3>Result</h3>
<p>E. faecalis dominated in oral infections compared with E. faecium. Isolates were susceptible to most antibiotics (only four were resistant to vancomycin). To our knowledge, the first report from this region.</p>
</sec>
<sec id="j_1905-7415.1001.464_s_005">
<h3>Conclusion</h3>
<p>Treatment of enterococcal infections of the oral mucosal and deep oral areas necessitate microbiological diagnosis and identification of resistance strains.</p>
</sec>
</abstract>

Background
Enterococci comprise a small share of oral flora and occur as contaminants in food such as meat and cheese. They are commonly encountered in patients with infections of oral tissues such as necrotic pulp, root canals and periodontitis.


Objective
To characterize the genotypes of Enterococcus isolated from patients with oral infection and to determine their antibiotic susceptibility.


Method
We genotypically characterized a number of isolates of Enterococcus species derived from patients with various oral infections. All the isolates were tested for the presence of putative virulence genes; efaA (gene for endocarditis), gelE (gene for gelatinase), ace (gene for collagen binding antigen), asa (gene for aggregation substance), cylA (gene for cytolysin activator), and esp (gene for surface adhesin) of E. faecalis and E. faecium as described in previous studies.


Result
E. faecalis dominated in oral infections compared with E. faecium. Isolates were susceptible to most antibiotics (only four were resistant to vancomycin). To our knowledge, the first report from this region.


Conclusion
Treatment of enterococcal infections of the oral mucosal and deep oral areas necessitate microbiological diagnosis and identification of resistance strains.

Genotypic characterization of <italic>Enterococcus</italic> species isolated from the oral cavity and their pattern of antibiotic susceptibility

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Genotypic characterization of  species isolated from the oral cavity and their pattern of antibiotic susceptibility

Background
Enterococci comprise a small share of oral flora and occur as contaminants in food such as meat and cheese. They are commonly encountered in...

Gene	Primers Sequence 5’-3’	Product	References
E. faecalis	5’-ATCAAGTACAGTTAGTCT-3’	941	[3]
	5’-ACGATTCAAAGCTAACTG-3’
E.faecium	5’-TTGAGGCAGACCAGATTGACG-3’	685	[3]
	5’-TATGACAGCGACTCCGATTCC-3’
gelE	5’-ACCCCGTATCATTGGTTT-3’	419	[9]
	5’-ACGCATTGCTTTTCCATC-3’
esp	5’-TTGCTAATGCTAGTCCACGACC-3’	932	[8]
	5’-GCGTCAACACTTGCATTGCCGA-3’
cylA	S’-GACTCGGGGATTGATAGGC-S’	688	[8]
	5’-GCTGCTAAAGCTGCGCTTAC-3’
ace	5’-GGAATGACCGAGAACGATGGC-3’	616	[8]
	5’-GCTTGATGTTGGCCTGCTTCCG-3’
efaA	5’-GCCAArTGGGACAGACCCTC-3’	688	[8]
	5’-CGCCTTCTGTTCCTTCrTTGGC-3’

Genotypic characterization of Enterococcus species isolated from the oral cavity and their pattern of antibiotic susceptibility

Article Category: Brief communication (Original)

Published Online: Jan 31, 2017

Page range: 49 - 53

DOI: https://doi.org/10.5372/1905-7415.1001.464

Keywords
Antibiotic susceptibility pattern, Enterococcus species, genotypic characterization, oral infections

© 2016 Bangalore H. Durgesh, Abdulaziz A. Alkheraif, Asmaa M. Malash, Mohamed I. Hashem, Mansour K. Assery, Mohammed Al Asmari, Pavithra Durgesh, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Primers used to identify species and to detect the virulence genes

Genotypic characterization of Enterococcus species isolated from the oral cavity and their pattern of antibiotic susceptibility

Article Category: Brief communication (Original)

Published Online: Jan 31, 2017

Page range: 49 - 53

DOI: https://doi.org/10.5372/1905-7415.1001.464

KeywordsAntibiotic susceptibility pattern, Enterococcus species, genotypic characterization, oral infections

© 2016 Bangalore H. Durgesh, Abdulaziz A. Alkheraif, Asmaa M. Malash, Mohamed I. Hashem, Mansour K. Assery, Mohammed Al Asmari, Pavithra Durgesh, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Primers used to identify species and to detect the virulence genes

Keywords
Antibiotic susceptibility pattern, Enterococcus species, genotypic characterization, oral infections