<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: In the last years, Leptospira spp Ballum has increased its representation in human clinical isolations in Cuba. Effective vaccines are needed to control this zoonotic disease.Objective: With the objective of developing a new vaccine candidate able to generate an effective protection against this serovar, two monovalent formulations developed by two highly virulent strains were evaluated (FoBa and FoBb).Methods: Clinical isolates of Leptospira serovar Ballum were subjected to serial passages in hamsters and monovalent vaccines were produced by modified methods developed for vax-SPIRAL®. The vaccine efficacy was tested in both experimental and control hamsters.Results: The Mesocricetus auratus biomodel showed that both formulations generated a protection of 100% against the Ballum lethal infection together to high levels of IgG antibodies and were efficient in the elimination of homologous carrier state but not heterologous carrier states.Conclusion: Both FoBa and FoBb vaccines were protective against leptospirosis with high IgG titers, absence of clinical signs and dead, and absence of leptospires in kidney of sacrificed animals.</abstract>

Background: In the last years, Leptospira spp Ballum has increased its representation in human clinical isolations in Cuba. Effective vaccines are needed to control this zoonotic disease.Objective: With the objective of developing a new vaccine candidate able to generate an effective protection against this serovar, two monovalent formulations developed by two highly virulent strains were evaluated (FoBa and FoBb).Methods: Clinical isolates of Leptospira serovar Ballum were subjected to serial passages in hamsters and monovalent vaccines were produced by modified methods developed for vax-SPIRAL®. The vaccine efficacy was tested in both experimental and control hamsters.Results: The Mesocricetus auratus biomodel showed that both formulations generated a protection of 100% against the Ballum lethal infection together to high levels of IgG antibodies and were efficient in the elimination of homologous carrier state but not heterologous carrier states.Conclusion: Both FoBa and FoBb vaccines were protective against leptospirosis with high IgG titers, absence of clinical signs and dead, and absence of leptospires in kidney of sacrificed animals.

Original article. Inmunoprotector potential of cellular vaccine formulations developed from Leptospira interrogans Ballum using Mesocricetus auratus as biomodel

Institute of Pharmacy and Food Science, University of Havana, Agrarian University of Havana (UNAH), Mayabeque, Cuba

Research Vice president (Finlay Institute), Havana, Agrarian University of Havana (UNAH), Mayabeque,

Veterinary Medicine Faculty, Agrarian University of Havana (UNAH), Mayabeque,

Institute of Pharmacy and Food Science, University of Havana, Agrarian University of Havana (UNAH), Mayabeque,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: In the last years, Leptospira spp Ballum has increased its representation in human clinical isolations in Cuba. Effective vaccines are needed to...

Original article. Inmunoprotector potential of cellular vaccine formulations developed from Leptospira interrogans Ballum using Mesocricetus auratus as biomodel

Published Online: Feb 04, 2017

Page range: 825 - 832

DOI: https://doi.org/10.5372/1905-7415.0606.128

Keywords
Leptospira, leptospirosis, Mesocricetus auratus, vaccine

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Original article. Inmunoprotector potential of cellular vaccine formulations developed from Leptospira interrogans Ballum using Mesocricetus auratus as biomodel

Published Online: Feb 04, 2017

Page range: 825 - 832

DOI: https://doi.org/10.5372/1905-7415.0606.128

KeywordsLeptospira, leptospirosis, Mesocricetus auratus, vaccine

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Leptospira, leptospirosis, Mesocricetus auratus, vaccine