<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Significantly lower LINE-1 methylation levels have been observed in several cancers compared to their normal cell counterparts. It is of interest to note that this epigenetic phenomenon is rather common to cancers with multistage oncogenesis. Objective: We compared LINE-1 methylation levels of cancer cells between the primary and matched metastatic sites. Methods: COBRA method was used to determine LINE-1 methylation levels of tumor cells at the primary and matched metastatic sites in five malignancies (10 cases each of head and neck squamous carcinoma, papillary thyroid carcinoma, non-small cell lung cancer, invasive mammary ductal carcinoma, and colorectal adenocarcinoma). LINE-1 methylation levels between the primary and matched metastatic site were compared. Results: Significantly lower LINE-1 methylation levels were observed at metastatic sites (as compared to primary sites) in head and neck squamous cell carcinoma (p = 0.002) and non-small cell lung cancer (p = 0.012). Conclusion: In head, neck, and pulmonary cancers, significantly lower LINE-1 methylation levels were found at metastatic sites, as compared to the matched primary tumor. Further studies are warranted to determine whether this altered methylation is the result or the cause of cancer metastasis.</abstract>

Background: Significantly lower LINE-1 methylation levels have been observed in several cancers compared to their normal cell counterparts. It is of interest to note that this epigenetic phenomenon is rather common to cancers with multistage oncogenesis. Objective: We compared LINE-1 methylation levels of cancer cells between the primary and matched metastatic sites. Methods: COBRA method was used to determine LINE-1 methylation levels of tumor cells at the primary and matched metastatic sites in five malignancies (10 cases each of head and neck squamous carcinoma, papillary thyroid carcinoma, non-small cell lung cancer, invasive mammary ductal carcinoma, and colorectal adenocarcinoma). LINE-1 methylation levels between the primary and matched metastatic site were compared. Results: Significantly lower LINE-1 methylation levels were observed at metastatic sites (as compared to primary sites) in head and neck squamous cell carcinoma (p = 0.002) and non-small cell lung cancer (p = 0.012). Conclusion: In head, neck, and pulmonary cancers, significantly lower LINE-1 methylation levels were found at metastatic sites, as compared to the matched primary tumor. Further studies are warranted to determine whether this altered methylation is the result or the cause of cancer metastasis.

Changes in LINE-1 methylation level in cancers during metastasis

Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Center of Excellence in Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

Chulalongkorn GenePRO Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Changes in LINE-1 methylation level in cancers during metastasis"}

Background: Significantly lower LINE-1 methylation levels have been observed in several cancers compared to their normal cell counterparts. It is of interest...

Changes in LINE-1 methylation level in cancers during metastasis

Article Category: Brief communication (Original)

Published Online: Feb 04, 2017

Page range: 307 - 310

DOI: https://doi.org/10.5372/1905-7415.0602.059

Keywords
LINE-1 methylation, metastasis, multi-step oncogenesis, primary tumor, tumor progression

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Changes in LINE-1 methylation level in cancers during metastasis

Article Category: Brief communication (Original)

Published Online: Feb 04, 2017

Page range: 307 - 310

DOI: https://doi.org/10.5372/1905-7415.0602.059

KeywordsLINE-1 methylation, metastasis, multi-step oncogenesis, primary tumor, tumor progression

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
LINE-1 methylation, metastasis, multi-step oncogenesis, primary tumor, tumor progression