<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Ablizia julibrissin could inhibit the proliferation of cancer cells in vitro and in vivo. However, the effects of the compositions Julibroside J8 and J12 on the angiogenesis are still poorly understood. Objective: We compared the pharmacodynamics of julibroside J8 and J12 in inhibiting angiogenesis in vitro and in vivo. Methods: Julibroside J8 and J12 were separated from the crude extract of Ablizia julibrissin. The effects of julibroside J8 and julibroside J12 on growth, migration, and matrigel tube formation of human microvascular endothelial cells (HMEC-1) were explored. In addition, the in vivo anti-angiogenic effect of julibroside J8 and J12 was evaluated on a chorioallantoic membrane (CAM). Results: HMEC-1 cells showed dose-dependent inhibition of growth, migration, and tube formation, when treated with 0.5-4 μg/ml julibroside J8 or 0.1-0.5 μg/ml julibroside J12 respectively. The formation of microvessels on CAM was also inhibited by julibroside J8 or julibroside J12 at concentrations of 10-100 μg.egg-1 or 5-50 μg.egg-1 respectively. Conclusion: Julibroside J12 confers more potent inhibitory effect on angiogenesis than julibroside J8 does.</abstract>

Background: Ablizia julibrissin could inhibit the proliferation of cancer cells in vitro and in vivo. However, the effects of the compositions Julibroside J8 and J12 on the angiogenesis are still poorly understood. Objective: We compared the pharmacodynamics of julibroside J8 and J12 in inhibiting angiogenesis in vitro and in vivo. Methods: Julibroside J8 and J12 were separated from the crude extract of Ablizia julibrissin. The effects of julibroside J8 and julibroside J12 on growth, migration, and matrigel tube formation of human microvascular endothelial cells (HMEC-1) were explored. In addition, the in vivo anti-angiogenic effect of julibroside J8 and J12 was evaluated on a chorioallantoic membrane (CAM). Results: HMEC-1 cells showed dose-dependent inhibition of growth, migration, and tube formation, when treated with 0.5-4 μg/ml julibroside J8 or 0.1-0.5 μg/ml julibroside J12 respectively. The formation of microvessels on CAM was also inhibited by julibroside J8 or julibroside J12 at concentrations of 10-100 μg.egg-1 or 5-50 μg.egg-1 respectively. Conclusion: Julibroside J12 confers more potent inhibitory effect on angiogenesis than julibroside J8 does.

Pharmacodynamics of julibroside J8 and J12 in inhibiting angiogenesis

Wuxi Higher Health Vocational Technology School, Wuxi 214028,

Department of Nuclear Medicine, Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai 200072,

Shanghai Lingwei Medical Technology Co. Ltd, Shanghai 200025,

PhD, Department of Nuclear Medicine, Shanghai 10th People’s Hospital, Tongji University School of Medicine, Shanghai 200072,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Pharmacodynamics of julibroside J and J in inhibiting angiogenesis

Background: Ablizia julibrissin could inhibit the proliferation of cancer cells in vitro and in vivo. However, the effects of the compositions Julibroside J8...

Pharmacodynamics of julibroside J₈ and J₁₂ in inhibiting angiogenesis

Article Category: Original article

Published Online: Feb 04, 2017

Page range: 755 - 764

DOI: https://doi.org/10.5372/1905-7415.0506.102

Keywords
Angiogenesis, chicken chorioallantoic membrane, human microvascular endothelial cells, julibroside J8, julibroside J12

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Pharmacodynamics of julibroside J8 and J12 in inhibiting angiogenesis

Article Category: Original article

Published Online: Feb 04, 2017

Page range: 755 - 764

DOI: https://doi.org/10.5372/1905-7415.0506.102

KeywordsAngiogenesis, chicken chorioallantoic membrane, human microvascular endothelial cells, julibroside J8, julibroside J12

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Pharmacodynamics of julibroside J₈ and J₁₂ in inhibiting angiogenesis

Keywords
Angiogenesis, chicken chorioallantoic membrane, human microvascular endothelial cells, julibroside J8, julibroside J12