<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Up-regulation of interferon alpha (IFN-α) in cutaneous lesions of lupus erythematosus (LE) has been proposed to play a role in the pathogenesis of cutaneous LE lesions of LE patients. IFN- α is composed of 13 subtypes of immunoregulatory cytokines that promote innate and adaptive immune responses. However, the precise biological properties may differ among subtypes.Objective: To investigate the mRNA level of IFN-α and subtypes in cutaneous LE patientsMethods: We analyzed the mRNA level of IFN-α and subtypes in normal skin from 12 healthy female controls and compared it to normal skin and discoid lupus erythematosus (DLE) lesions from 16 DLE patients without systemic involvement (14 females and 2 males) by real-time PCR, cloning, and sequencing.Results: Significant up-regulation of mRNA level of IFN-α was found in both lesional and non-lesional skin from DLE patients without SLE when compared to normal skin from healthy donors (P=0.05 and P=0.04, respectively). An analysis of the IFN-α subtypes from skin biopsies detected a reduction of IFN-α subtype 5 in DLE lesion compared to healthy control skin.Conclusions: Our results indicate that IFN- α is upregulated in both lesional and non-lesional DLE in cutaneous LE patients. IFN-α subtype 5 is the main subtype of IFN- α expression in normal skin but declines in DLE.</abstract>

Background: Up-regulation of interferon alpha (IFN-α) in cutaneous lesions of lupus erythematosus (LE) has been proposed to play a role in the pathogenesis of cutaneous LE lesions of LE patients. IFN- α is composed of 13 subtypes of immunoregulatory cytokines that promote innate and adaptive immune responses. However, the precise biological properties may differ among subtypes.Objective: To investigate the mRNA level of IFN-α and subtypes in cutaneous LE patientsMethods: We analyzed the mRNA level of IFN-α and subtypes in normal skin from 12 healthy female controls and compared it to normal skin and discoid lupus erythematosus (DLE) lesions from 16 DLE patients without systemic involvement (14 females and 2 males) by real-time PCR, cloning, and sequencing.Results: Significant up-regulation of mRNA level of IFN-α was found in both lesional and non-lesional skin from DLE patients without SLE when compared to normal skin from healthy donors (P=0.05 and P=0.04, respectively). An analysis of the IFN-α subtypes from skin biopsies detected a reduction of IFN-α subtype 5 in DLE lesion compared to healthy control skin.Conclusions: Our results indicate that IFN- α is upregulated in both lesional and non-lesional DLE in cutaneous LE patients. IFN-α subtype 5 is the main subtype of IFN- α expression in normal skin but declines in DLE.

Interferon Alpha mRNA level and subtypes in lesion and non-lesion from discoid lupus erythematosus patients without systemic lupus erythematosus

MD, PhD, Department of Microbiology, Division of Immunology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

National Institute of Health, Department of Medical Sciences, Nonthaburi 11000,

Department of Medicine, Division of Dermatology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Department of Microbiology, Division of Immunology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Background: Up-regulation of interferon alpha (IFN-α) in cutaneous lesions of lupus erythematosus (LE) has been proposed to play a role in the pathogenesis of...

Interferon Alpha mRNA level and subtypes in lesion and non-lesion from discoid lupus erythematosus patients without systemic lupus erythematosus

Article Category: Original article

Published Online: Feb 04, 2017

Page range: 643 - 647

DOI: https://doi.org/10.5372/1905-7415.0505.085

Keywords
Cutaneous LE patients, DLE, interferon-alpha, mRNA, subtype

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Interferon Alpha mRNA level and subtypes in lesion and non-lesion from discoid lupus erythematosus patients without systemic lupus erythematosus

Article Category: Original article

Published Online: Feb 04, 2017

Page range: 643 - 647

DOI: https://doi.org/10.5372/1905-7415.0505.085

KeywordsCutaneous LE patients, DLE, interferon-alpha, mRNA, subtype

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Keywords
Cutaneous LE patients, DLE, interferon-alpha, mRNA, subtype