<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jmotherandchild.20202404.d-20-00014_s_008">
<h3>Background</h3>
<p>There are limited data on factors that determine viral load (VL) in congenital cytomegalovirus (cCMV) infection. Single nucleotide polymorphisms (SNPs) might influence individual host response to infection. This study aimed to investigate the association between SNPs in genes encoding cytokines or cytokine receptors and VL in newborns with cCMV.</p>
</sec>
<sec id="j_jmotherandchild.20202404.d-20-00014_s_009">
<h3>Material and methods</h3>
<p>Eight polymorphisms (IL1B rs16944, IL12B rs3212227, IL28B rs12979860, CCL2 rs1024611, DC-SIGN rs735240, TLR2 rs5743708, TLR4 rs4986791 and TLR9 rs352140) were analyzed in study population of 233 newborns, including 92 cCMV-infected newborns (73 symptomatic and 19 asymptomatic) by TaqMan SNP Predesigned Genotyping Assays. The association analysis was performed using SNPStats software and STATISTICA10.</p>
</sec>
<sec id="j_jmotherandchild.20202404.d-20-00014_s_010">
<h3>Results</h3>
<p>The association between IL12B polymorphism and viruria was observed (<italic>p</italic> = 0.029). In multiple comparison tests, heterozygous T/G genotype of IL12B was associated with higher viruria than T/T genotype (<italic>p</italic> = 0.041) in cCMV-infected newborns. In allele analysis, T allele of IL12B was associated with higher viremia (<italic>p</italic> = 0.037) in symptomatic newborns. We observed higher VL in symptomatic newborns in comparison to asymptomatic (median viremia: 1.7 × 10<sup>4</sup> copies/mL vs. 2.0 × 10<sup>3</sup> copies/mL (<italic>p</italic> = 0.002), median viruria: 1.0 × 10<sup>7</sup> copies/mL versus 6.9 × 10<sup>5</sup> copies/mL (<italic>p</italic> = 0.001), respectively).</p>
</sec>
<sec id="j_jmotherandchild.20202404.d-20-00014_s_011">
<h3>Conclusions</h3>
<p>IL12B rs3212227 was associated with VL in cCMV. Symptomatic newborns had significantly higher viremia and viruria. The role of SNPs in pathogenesis of cCMV warrants further investigations.</p>
</sec>
</abstract>

Background
There are limited data on factors that determine viral load (VL) in congenital cytomegalovirus (cCMV) infection. Single nucleotide polymorphisms (SNPs) might influence individual host response to infection. This study aimed to investigate the association between SNPs in genes encoding cytokines or cytokine receptors and VL in newborns with cCMV.


Material and methods
Eight polymorphisms (IL1B rs16944, IL12B rs3212227, IL28B rs12979860, CCL2 rs1024611, DC-SIGN rs735240, TLR2 rs5743708, TLR4 rs4986791 and TLR9 rs352140) were analyzed in study population of 233 newborns, including 92 cCMV-infected newborns (73 symptomatic and 19 asymptomatic) by TaqMan SNP Predesigned Genotyping Assays. The association analysis was performed using SNPStats software and STATISTICA10.


Results
The association between IL12B polymorphism and viruria was observed (p = 0.029). In multiple comparison tests, heterozygous T/G genotype of IL12B was associated with higher viruria than T/T genotype (p = 0.041) in cCMV-infected newborns. In allele analysis, T allele of IL12B was associated with higher viremia (p = 0.037) in symptomatic newborns. We observed higher VL in symptomatic newborns in comparison to asymptomatic (median viremia: 1.7 × 104 copies/mL vs. 2.0 × 103 copies/mL (p = 0.002), median viruria: 1.0 × 107 copies/mL versus 6.9 × 105 copies/mL (p = 0.001), respectively).


Conclusions
IL12B rs3212227 was associated with VL in cCMV. Symptomatic newborns had significantly higher viremia and viruria. The role of SNPs in pathogenesis of cCMV warrants further investigations.

Association between single nucleotide polymorphisms and viral load in congenital cytomegalovirus infection

Journal of Mother and Child

This work is licensed under the Creative Commons Attribution 4.0 International License.

Background
There are limited data on factors that determine viral load (VL) in congenital cytomegalovirus (cCMV) infection. Single nucleotide polymorphisms...

Association between single nucleotide polymorphisms and viral load in congenital cytomegalovirus infection

Article Category: Original article

Published Online: Jul 16, 2021

Page range: 9 - 17

DOI: https://doi.org/10.34763/jmotherandchild.20202404.d-20-00014

Keywords
viral load, single nucleotide polymorphism, congenital cytomegalovirus infection, newborn

© 2021 Dominika Jedlińska-Pijanowska et al. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Association between single nucleotide polymorphisms and viral load in congenital cytomegalovirus infection

Article Category: Original article

Published Online: Jul 16, 2021

Page range: 9 - 17

DOI: https://doi.org/10.34763/jmotherandchild.20202404.d-20-00014

Keywordsviral load, single nucleotide polymorphism, congenital cytomegalovirus infection, newborn

© 2021 Dominika Jedlińska-Pijanowska et al. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
viral load, single nucleotide polymorphism, congenital cytomegalovirus infection, newborn