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<p>Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the antagonistic effect of <italic>Bacillus subtilis</italic> CTXW 7-6-2 against <italic>Rhizoctonia solani</italic>, its ability to promote the growth of tobacco seedlings, and the expression of disease resistance-related genes for efficient and eco-friendly plant disease control. Our results showed that CTXW 7-6-2 had the most vigorous growth after being cultured for 96 h, and its rate of inhibition of <italic>R. solani</italic> growth <italic>in vitro</italic> was 94.02%. The volatile compounds produced by CTXW 7-6-2 inhibited the growth of <italic>R. solani</italic> significantly (by 96.62%). The fungal growthinhibition rate of the <italic>B. subtilis</italic> CTXW 7-6-2 broth obtained after high-temperature and no-high-temperature sterile fermentation was low, at 50.88% and 54.63%, respectively. The lipopeptides extracted from the <italic>B. subtilis</italic> CTXW 7-6-2 fermentation broth showed a 74.88% fungal growth inhibition rate at a concentration of 100 mg/l. Scanning and transmission electron microscopy showed some organelle structural abnormalities, collapse, shrinkage, blurring, and dissolution in the <italic>R. solani</italic> mycelia. In addition, CTXW 7-6-2 increased tobacco seedling growth and improved leaf and root weight compared to the control. After CTXW 7-6-2 inoculation, tobacco leaves showed the upregulation of the <italic>PDF1.2</italic>, <italic>PPO</italic>, and <italic>PAL</italic> genes, which are closely related to target spot disease resistance. In conclusion, <italic>B. subtilis</italic> CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.</p>
</abstract>

Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the antagonistic effect of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani, its ability to promote the growth of tobacco seedlings, and the expression of disease resistance-related genes for efficient and eco-friendly plant disease control. Our results showed that CTXW 7-6-2 had the most vigorous growth after being cultured for 96 h, and its rate of inhibition of R. solani growth in vitro was 94.02%. The volatile compounds produced by CTXW 7-6-2 inhibited the growth of R. solani significantly (by 96.62%). The fungal growthinhibition rate of the B. subtilis CTXW 7-6-2 broth obtained after high-temperature and no-high-temperature sterile fermentation was low, at 50.88% and 54.63%, respectively. The lipopeptides extracted from the B. subtilis CTXW 7-6-2 fermentation broth showed a 74.88% fungal growth inhibition rate at a concentration of 100 mg/l. Scanning and transmission electron microscopy showed some organelle structural abnormalities, collapse, shrinkage, blurring, and dissolution in the R. solani mycelia. In addition, CTXW 7-6-2 increased tobacco seedling growth and improved leaf and root weight compared to the control. After CTXW 7-6-2 inoculation, tobacco leaves showed the upregulation of the PDF1.2, PPO, and PAL genes, which are closely related to target spot disease resistance. In conclusion, B. subtilis CTXW 7-6-2 may be an efficient biological control agent in tobacco agriculture and enhance plant growth potential.

Biocontrol and Growth Promotion Potential of <italic>Bacillus subtilis</italic> CTXW 7-6-2 against <italic>Rhizoctonia solani</italic> that Causes Tobacco Target Spot Disease

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Biocontrol and Growth Promotion Potential of  CTXW 7-6-2 against  that Causes Tobacco Target Spot Disease

Fungal diseases form perforated disease spots in tobacco plants, resulting in a decline in tobacco yield and quality. The present study investigated the...

Biocontrol and Growth Promotion Potential of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani that Causes Tobacco Target Spot Disease

Article Category: ORIGINAL PAPER

Published Online: Mar 04, 2024

Page range: 29 - 38

Received: Sep 15, 2023

Accepted: Dec 19, 2023

DOI: https://doi.org/10.33073/pjm-2024-004

Keywords
tobacco target spot, plant growth promotion, biocontrol potential

© 2024 Ning Huang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Biocontrol and Growth Promotion Potential of Bacillus subtilis CTXW 7-6-2 against Rhizoctonia solani that Causes Tobacco Target Spot Disease

Article Category: ORIGINAL PAPER

Published Online: Mar 04, 2024

Page range: 29 - 38

Received: Sep 15, 2023

Accepted: Dec 19, 2023

DOI: https://doi.org/10.33073/pjm-2024-004

Keywordstobacco target spot, plant growth promotion, biocontrol potential

© 2024 Ning Huang et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
tobacco target spot, plant growth promotion, biocontrol potential