<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The <italic>Clostridium perfringens</italic> bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal contamination. Tests determining <italic>C. perfringens</italic> in water samples are usually performed by membrane filtration where filters are incubated on selective media under anaerobic conditions. Available media include mCP and TSC. The aim of this study was to compare the relative recovery of <italic>C. perfringens</italic> (including spores) from surface water samples and to determine the performance characteristics of the membrane filtration method using both media. The results showed that, although the procedure using the mCP medium was more sensitive and specific, higher recoveries were obtained in the tests based on the TSC medium.
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<graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2023-039_fig_001.jpg"></graphic>
</fig></p>
</abstract>

The Clostridium perfringens bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal contamination. Tests determining C. perfringens in water samples are usually performed by membrane filtration where filters are incubated on selective media under anaerobic conditions. Available media include mCP and TSC. The aim of this study was to compare the relative recovery of C. perfringens (including spores) from surface water samples and to determine the performance characteristics of the membrane filtration method using both media. The results showed that, although the procedure using the mCP medium was more sensitive and specific, higher recoveries were obtained in the tests based on the TSC medium.

Comparison of mCP and TSC Media to Enumerate <italic>Clostridium perfringens</italic> in Surface Water Samples

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Comparison of mCP and TSC Media to Enumerate  in Surface Water Samples

The Clostridium perfringens bacteria are used to assess water quality as an indicator parameter. If detected, it can confirm the occurrence of past fecal...

Comparison of mCP and TSC Media to Enumerate Clostridium perfringens in Surface Water Samples

Article Category: ORIGINAL PAPER

Published Online: Dec 16, 2023

Page range: 413 - 419

Received: Jun 07, 2023

Accepted: Sep 26, 2023

DOI: https://doi.org/10.33073/pjm-2023-039

Keywords
mCP medium, membrane filtration, surface water, TSC medium

© 2023 Renata Matuszewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Comparison of mCP and TSC Media to Enumerate Clostridium perfringens in Surface Water Samples

Article Category: ORIGINAL PAPER

Published Online: Dec 16, 2023

Page range: 413 - 419

Received: Jun 07, 2023

Accepted: Sep 26, 2023

DOI: https://doi.org/10.33073/pjm-2023-039

KeywordsmCP medium, membrane filtration, surface water, TSC medium

© 2023 Renata Matuszewska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
mCP medium, membrane filtration, surface water, TSC medium