<abstract xmlns="http://www.w3.org/1999/xhtml">

This work investigated the genetic relationship among <italic>Stenotrophomonas maltophilia</italic> strains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the isolates were identified with MALDI-TOF/MS. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 platform (Illumina, Inc., USA). The antimicrobial resistance genes were detected using CGE services. The phylogenetic analysis of <italic>S. maltophilia</italic> strains was performed by Roary and MEGA X. In total, 24 <italic>S. maltophilia</italic> isolates were isolated. The results of resistome analysis showed all <italic>S. maltophilia</italic> strains carrying <italic>bla</italic>L1 gene, which was the only β-lactamase genotype. In addition, the aminoglycoside resistance genes <italic>aac(6′)-Iz</italic> and <italic>aph(3′)-IIc</italic> were found. The phylogenetic tree indicated the clonal diversity of <italic>S. maltophilia</italic> in these two regions and the clonal relatedness of the strains from these regions. This study first investigated the dissemination and characterization of <italic>S. maltophilia</italic> isolates from dairy cows in northeast China and provided evidence of the potential transmission between two provinces. Furthermore, it indicated <italic>bla</italic>L1 was the most prevalent genotype of β-lactamase in these regions.
</abstract>

This work investigated the genetic relationship among Stenotrophomonas maltophilia strains in fecal samples from dairy cows in northeast China and identified the dominant β-lactamase genotype. One hundred and six samples were collected from two randomly selected cow farms in northeast China, and the isolates were identified with MALDI-TOF/MS. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 platform (Illumina, Inc., USA). The antimicrobial resistance genes were detected using CGE services. The phylogenetic analysis of S. maltophilia strains was performed by Roary and MEGA X. In total, 24 S. maltophilia isolates were isolated. The results of resistome analysis showed all S. maltophilia strains carrying blaL1 gene, which was the only β-lactamase genotype. In addition, the aminoglycoside resistance genes aac(6′)-Iz and aph(3′)-IIc were found. The phylogenetic tree indicated the clonal diversity of S. maltophilia in these two regions and the clonal relatedness of the strains from these regions. This study first investigated the dissemination and characterization of S. maltophilia isolates from dairy cows in northeast China and provided evidence of the potential transmission between two provinces. Furthermore, it indicated blaL1 was the most prevalent genotype of β-lactamase in these regions.

Dissemination and characterization of <italic>Stenotrophomonas maltophilia</italic> isolates from Dairy Cows in Northeast China

The Third Affiliated Hospital of Zhejiang Traditional Chinese Medical University

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital

Jinan Microecological Biomedicine Shandong Laboratory

Department of Medical Oncology, The First Affiliated Hospital

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Dissemination and characterization of  isolates from Dairy Cows in Northeast China

This work investigated the genetic relationship among Stenotrophomonas maltophilia strains in fecal samples from dairy cows in northeast China and identified...

Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China

Article Category: Original Paper

Published Online: Sep 20, 2023

Page range: 319 - 323

Received: May 13, 2023

Accepted: Jul 14, 2023

DOI: https://doi.org/10.33073/pjm-2023-032

Keywords
gene, Whole-genome sequencing, phylogenetic analysis

© 2023 Lanjun Shao et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Dissemination and characterization of Stenotrophomonas maltophilia isolates from Dairy Cows in Northeast China

Article Category: Original Paper

Published Online: Sep 20, 2023

Page range: 319 - 323

Received: May 13, 2023

Accepted: Jul 14, 2023

DOI: https://doi.org/10.33073/pjm-2023-032

Keywordsgene, Whole-genome sequencing, phylogenetic analysis

© 2023 Lanjun Shao et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
gene, Whole-genome sequencing, phylogenetic analysis