<abstract xmlns="http://www.w3.org/1999/xhtml">

The prokaryotic microalga <italic>Limnothrix redekei</italic> KNUA012 isolated from a freshwater bloom sample from Lake Hapcheon, Hapcheon-gun, South Korea, was investigated for its potential as a biofuel feedstock. Microalgae produce straight-chain alkanes/alkenes from acyl carrier protein-linked fatty acyls via aldehyde decarbonylase (AD; EC 1.2.1.3), which can convert aldehyde intermediates into various biofuel precursors, such as alkanes and free fatty acids. In <italic>L. redekei</italic> KNUA012, long-chain ADs can convert fatty aldehyde intermediates into alkanes. After heterologous AD expression in <italic>Escherichia coli</italic> (pET28-AD), we identified an AD in <italic>L. redekei</italic> KNUA012 that can synthesize various alkanes, such as pentadecane (C15H32), 8-heptadecene (C17H34), and heptadecane (C17H36). These alkanes can be directly used as fuels without transesterification. Biodiesel constituents including dodecanoic acid (C13H26O2), tetradecanoic acid (C15H30O2), 9-hexa decenoic acid (C17H32O2), palmitoleic acid (C17H32O2), hexadecanoic acid (C17H34O2), 9-octadecenoic acid (C19H36O2), and octadecanoic acid (C19H38O2) are produced by <italic>L. redekei</italic> KNUA012 as the major fatty acids. Our findings suggest that Korean domestic <italic>L. redekei</italic> KNUA012 is a promising resource for microalgae-based biofuels and biofuel feedstock.
</abstract>

The prokaryotic microalga Limnothrix redekei KNUA012 isolated from a freshwater bloom sample from Lake Hapcheon, Hapcheon-gun, South Korea, was investigated for its potential as a biofuel feedstock. Microalgae produce straight-chain alkanes/alkenes from acyl carrier protein-linked fatty acyls via aldehyde decarbonylase (AD; EC 1.2.1.3), which can convert aldehyde intermediates into various biofuel precursors, such as alkanes and free fatty acids. In L. redekei KNUA012, long-chain ADs can convert fatty aldehyde intermediates into alkanes. After heterologous AD expression in Escherichia coli (pET28-AD), we identified an AD in L. redekei KNUA012 that can synthesize various alkanes, such as pentadecane (C15H32), 8-heptadecene (C17H34), and heptadecane (C17H36). These alkanes can be directly used as fuels without transesterification. Biodiesel constituents including dodecanoic acid (C13H26O2), tetradecanoic acid (C15H30O2), 9-hexa decenoic acid (C17H32O2), palmitoleic acid (C17H32O2), hexadecanoic acid (C17H34O2), 9-octadecenoic acid (C19H36O2), and octadecanoic acid (C19H38O2) are produced by L. redekei KNUA012 as the major fatty acids. Our findings suggest that Korean domestic L. redekei KNUA012 is a promising resource for microalgae-based biofuels and biofuel feedstock.

The Prokaryotic Microalga <italic>Limnothrix redekei</italic> KNUA012 to Improve Aldehyde Decarbonylase Expression for Use as a Biological Resource

Research Institute of Ulleung-do and Dok-do

School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group

Department of Biology, College of Natural Sciences

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Prokaryotic Microalga  KNUA012 to Improve Aldehyde Decarbonylase Expression for Use as a Biological Resource

The prokaryotic microalga Limnothrix redekei KNUA012 isolated from a freshwater bloom sample from Lake Hapcheon, Hapcheon-gun, South Korea, was investigated...

The Prokaryotic Microalga Limnothrix redekei KNUA012 to Improve Aldehyde Decarbonylase Expression for Use as a Biological Resource

Article Category: Original Paper

Published Online: Sep 20, 2023

Page range: 307 - 317

Received: Mar 15, 2023

Accepted: Jul 04, 2023

DOI: https://doi.org/10.33073/pjm-2023-031

Keywords
alkanes, aldehyde decarbonylase, biofuels, biomass, fatty acid

© 2023 Young-Saeng Kim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Prokaryotic Microalga Limnothrix redekei KNUA012 to Improve Aldehyde Decarbonylase Expression for Use as a Biological Resource

Article Category: Original Paper

Published Online: Sep 20, 2023

Page range: 307 - 317

Received: Mar 15, 2023

Accepted: Jul 04, 2023

DOI: https://doi.org/10.33073/pjm-2023-031

Keywordsalkanes, aldehyde decarbonylase, biofuels, biomass, fatty acid

© 2023 Young-Saeng Kim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
alkanes, aldehyde decarbonylase, biofuels, biomass, fatty acid