<abstract xmlns="http://www.w3.org/1999/xhtml">

Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis syndrome. In this study, a uricase-producing strain (named CSAJ-16) was isolated from the soil sample of Cangshan Mountain, Yunnan Province, China. This strain was identified as <italic>Arthrobacter</italic> sp. CSAJ-16. Based on the gene sequence alignment, the uricase gene (named <italic>aruox</italic>) of <italic>Arthrobacter</italic> sp. CSAJ-16 was amplified and heterologously expressed. The recombinant uricase (ArUOX) was about 32 kDa. The optimal pH and temperature of ArUOX were pH 7 and 20°C, respectively. The ArUOX remained above 50% relative activity after incubation at 37°C for 100 min or at pH 6.0–8.6 for 24 h. Moreover, metal ions such as K+, Mg2+, Ca2+, Ba2+ and Pb2+ can significantly enhance the activity of ArUOX (> 200%). These enzymatic properties indicate that ArUOX has potential applications in pharmaceutical enzymes and uric acid detection kits.
</abstract>

Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis syndrome. In this study, a uricase-producing strain (named CSAJ-16) was isolated from the soil sample of Cangshan Mountain, Yunnan Province, China. This strain was identified as Arthrobacter sp. CSAJ-16. Based on the gene sequence alignment, the uricase gene (named aruox) of Arthrobacter sp. CSAJ-16 was amplified and heterologously expressed. The recombinant uricase (ArUOX) was about 32 kDa. The optimal pH and temperature of ArUOX were pH 7 and 20°C, respectively. The ArUOX remained above 50% relative activity after incubation at 37°C for 100 min or at pH 6.0–8.6 for 24 h. Moreover, metal ions such as K+, Mg2+, Ca2+, Ba2+ and Pb2+ can significantly enhance the activity of ArUOX (> 200%). These enzymatic properties indicate that ArUOX has potential applications in pharmaceutical enzymes and uric acid detection kits.

Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from <italic>Arthrobacter</italic> sp. CSAJ-16 in Cangshan Mountain

College of Agriculture and Biological Science

The First Affiliated Hospital of Kunming Medical University

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from  sp. CSAJ-16 in Cangshan Mountain

Uricase (or Urate oxidase), a key enzyme involved in purine metabolism, is commonly used in treating conditions such as gout, hyperuricemia, and tumor lysis...

Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain

Article Category: Original Paper

Published Online: Sep 20, 2023

Page range: 277 - 283

Received: Apr 10, 2023

Accepted: Jun 09, 2023

DOI: https://doi.org/10.33073/pjm-2023-027

Keywords
Cangshan Mountain, uricase-producing strain, sp., heterologous expression, neutral uricase

© 2023 Xin Yan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain

Article Category: Original Paper

Published Online: Sep 20, 2023

Page range: 277 - 283

Received: Apr 10, 2023

Accepted: Jun 09, 2023

DOI: https://doi.org/10.33073/pjm-2023-027

KeywordsCangshan Mountain, uricase-producing strain, sp., heterologous expression, neutral uricase

© 2023 Xin Yan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Keywords
Cangshan Mountain, uricase-producing strain, sp., heterologous expression, neutral uricase