<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Seventy-seven strains of <italic>Malassezia</italic> were included in this study. Biofilm and hydrolytic enzyme production were studied by using specific solid media. The Real-Time reverse transcriptase qPCR method was applied to determine the overexpression of genes encoding the extracellular enzymes. All included <italic>Malassezia</italic> species produced biofilms. No statistically significant difference was observed between <italic>Malassezia</italic> species in biofilm formation (<italic>p</italic> = 0.567). All <italic>Malassezia</italic> species produced lipase, and 95% of <italic>Malassezia globosa</italic> showed a strong enzymatic activity (Pz = 0.55 ± 0.02). A statistically significant difference was observed between the mean keratinase indices of <italic>Malassezia slooffiae</italic> and the other <italic>Malassezia</italic> species (<italic>p</italic> = 0.005). The overexpression of one or more genes was observed in 100% of strains isolated from patients with folliculitis, 87.5% – with pityriasis versicolor, and 57.14% of the control group isolates. A statistically significant difference in the lipase gene expression (<italic>p</italic> = 0.042) was between the strains from patients with folliculitis and the control group. This investigation provides more information about the frequency of the production of the major enzymes considered virulence factors of <italic>Malassezia</italic> species. Interestingly, the overexpression of one or more genes was observed in strains isolated from patients with <italic>Malassezia</italic> disorders.</p>
<p><inline-graphic xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="graphic/j_pjm-2022-047_eq_001.png"></inline-graphic></p>
</abstract>

Seventy-seven strains of Malassezia were included in this study. Biofilm and hydrolytic enzyme production were studied by using specific solid media. The Real-Time reverse transcriptase qPCR method was applied to determine the overexpression of genes encoding the extracellular enzymes. All included Malassezia species produced biofilms. No statistically significant difference was observed between Malassezia species in biofilm formation (p = 0.567). All Malassezia species produced lipase, and 95% of Malassezia globosa showed a strong enzymatic activity (Pz = 0.55 ± 0.02). A statistically significant difference was observed between the mean keratinase indices of Malassezia slooffiae and the other Malassezia species (p = 0.005). The overexpression of one or more genes was observed in 100% of strains isolated from patients with folliculitis, 87.5% – with pityriasis versicolor, and 57.14% of the control group isolates. A statistically significant difference in the lipase gene expression (p = 0.042) was between the strains from patients with folliculitis and the control group. This investigation provides more information about the frequency of the production of the major enzymes considered virulence factors of Malassezia species. Interestingly, the overexpression of one or more genes was observed in strains isolated from patients with Malassezia disorders.

Production and Quantification of Virulence Factors in <italic>Malassezia</italic> Species

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Production and Quantification of Virulence Factors in  Species

Seventy-seven strains of Malassezia were included in this study. Biofilm and hydrolytic enzyme production were studied by using specific solid media. The...

Production and Quantification of Virulence Factors in Malassezia Species

Article Category: Original Paper

Published Online: Dec 06, 2022

Page range: 529 - 538

Received: Jul 12, 2022

Accepted: Sep 20, 2022

DOI: https://doi.org/10.33073/pjm-2022-047

Keywords
Real-Time qRT-PCR, virulence factors, extracellular hydrolases, biofilm

© 2022 Inès Hadrich et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Production and Quantification of Virulence Factors in Malassezia Species

Article Category: Original Paper

Published Online: Dec 06, 2022

Page range: 529 - 538

Received: Jul 12, 2022

Accepted: Sep 20, 2022

DOI: https://doi.org/10.33073/pjm-2022-047

KeywordsReal-Time qRT-PCR, virulence factors, extracellular hydrolases, biofilm

© 2022 Inès Hadrich et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
Real-Time qRT-PCR, virulence factors, extracellular hydrolases, biofilm