<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This study aimed to investigate <italic>in vitro</italic> effects of the selected prebiotics alone, and in combination with two potential probiotic <italic>Lactobacillus</italic> strains on the microbial composition of <italic>Apis cerana</italic> gut microbiota and acid production. Four prebiotics, inulin, fructo-oligosaccharides, xylo-oligosaccharides, and isomalto-oligosaccharides were chosen, and glucose served as the carbon source. Supplementation of this four prebiotics increased numbers of <italic>Bifidobacterium</italic> and lactic acid bacteria while decreasing the pH value of <italic>in vitro</italic> fermentation broth inoculated with <italic>A. cerana</italic> gut microbiota compared to glucose. Then, two potential probiotics derived from <italic>A. cerana</italic> gut at different dosages, <italic>Lactobacillus helveticus</italic> KM7 and <italic>Limosilactobacillus reuteri</italic> LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with <italic>A. cerana</italic> gut microbiota, respectively. The most pronounced impact was observed with isomalto-oligosaccharides. Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of <italic>Bifidobacterium</italic>, LAB, and total bacteria and reduced the proliferation of <italic>Enterococcus</italic> and fungi. Consistent with these results, the altered metabolic activity was observed as lowered pH in <italic>in vitro</italic> culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains. The symbiotic impact varied with the types and concentration of <italic>Lactobacillus</italic> strains and fermentation time. The more effective ability was observed with IMO combined with <italic>L. helveticus</italic> KM7. These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on <italic>A. cerana</italic> gut microbiota.</p></abstract>

This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the microbial composition of Apis cerana gut microbiota and acid production. Four prebiotics, inulin, fructo-oligosaccharides, xylo-oligosaccharides, and isomalto-oligosaccharides were chosen, and glucose served as the carbon source. Supplementation of this four prebiotics increased numbers of Bifidobacterium and lactic acid bacteria while decreasing the pH value of in vitro fermentation broth inoculated with A. cerana gut microbiota compared to glucose. Then, two potential probiotics derived from A. cerana gut at different dosages, Lactobacillus helveticus KM7 and Limosilactobacillus reuteri LP4 were added with isomalto-oligosaccharides in fermentation broth inoculated with A. cerana gut microbiota, respectively. The most pronounced impact was observed with isomalto-oligosaccharides. Compared to isomalto-oligosaccharides alone, the combination of isomalto-oligosaccharides with both lactobacilli strains induced the growth of Bifidobacterium, LAB, and total bacteria and reduced the proliferation of Enterococcus and fungi. Consistent with these results, the altered metabolic activity was observed as lowered pH in in vitro culture of gut microbiota supplemented with isomalto-oligosaccharides and lactobacilli strains. The symbiotic impact varied with the types and concentration of Lactobacillus strains and fermentation time. The more effective ability was observed with IMO combined with L. helveticus KM7. These results suggested that isomalto-oligosaccharides could be a potential prebiotic and symbiotic with certain lactobacilli strains on A. cerana gut microbiota.

<italic>In vitro</italic> Effects of Prebiotics and Synbiotics on <italic>Apis cerana</italic> Gut Microbiota

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 Effects of Prebiotics and Synbiotics on  Gut Microbiota

This study aimed to investigate in vitro effects of the selected prebiotics alone, and in combination with two potential probiotic Lactobacillus strains on the...

In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota

Article Category: original-paper

Published Online: Dec 20, 2021

Page range: 511 - 520

Received: Oct 06, 2021

Accepted: Nov 27, 2021

DOI: https://doi.org/10.33073/pjm-2021-049

Keywords
isomalto-oligosaccharides, lactic acid bacteria, metabolic activity

© 2021 Mingkui Lv et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

In vitro Effects of Prebiotics and Synbiotics on Apis cerana Gut Microbiota

Article Category: original-paper

Published Online: Dec 20, 2021

Page range: 511 - 520

Received: Oct 06, 2021

Accepted: Nov 27, 2021

DOI: https://doi.org/10.33073/pjm-2021-049

Keywordsisomalto-oligosaccharides, lactic acid bacteria, metabolic activity

© 2021 Mingkui Lv et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Keywords
isomalto-oligosaccharides, lactic acid bacteria, metabolic activity