<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Latent pathogenic fungi (LPFs) affect plant growth, but some of them may stably colonize plants. LPFs were isolated from healthy <italic>Houttuynia cordata</italic> rhizomes to reveal this mechanism and identified as <italic>Ilyonectria liriodendri</italic>, an unidentified fungal sp., and <italic>Penicillium citrinum</italic>. Sterile <italic>H. cordata</italic> seedlings were cultivated in sterile or non-sterile soils and inoculated with the LPFs, followed by the plants’ analysis. The <italic>in vitro</italic> antifungal activity of <italic>H. cordata</italic> rhizome crude extracts on LPF were determined. The effect of inoculation of sterile seedlings by LPFs on the concentrations of rhizome phenolics was evaluated. The rates of <italic>in vitro</italic> growth inhibition amongst LPFs were determined. The LPFs had a strong negative effect on <italic>H. cordata</italic> in sterile soil; microbiota in non-sterile soil eliminated such influence. There was an interactive inhibition among LPFs; the secondary metabolites also regulated their colonization in <italic>H. cordata</italic> rhizomes. LPFs changed the accumulation of phenolics in <italic>H. cordata</italic>. The results provide that colonization of LPFs in rhizomes was regulated by the colonizing microbiota of <italic>H. cordata</italic>, the secondary metabolites in the <italic>H. cordata</italic> rhizomes, and the mutual inhibition and competition between the different latent pathogens.</p></abstract>

Latent pathogenic fungi (LPFs) affect plant growth, but some of them may stably colonize plants. LPFs were isolated from healthy Houttuynia cordata rhizomes to reveal this mechanism and identified as Ilyonectria liriodendri, an unidentified fungal sp., and Penicillium citrinum. Sterile H. cordata seedlings were cultivated in sterile or non-sterile soils and inoculated with the LPFs, followed by the plants’ analysis. The in vitro antifungal activity of H. cordata rhizome crude extracts on LPF were determined. The effect of inoculation of sterile seedlings by LPFs on the concentrations of rhizome phenolics was evaluated. The rates of in vitro growth inhibition amongst LPFs were determined. The LPFs had a strong negative effect on H. cordata in sterile soil; microbiota in non-sterile soil eliminated such influence. There was an interactive inhibition among LPFs; the secondary metabolites also regulated their colonization in H. cordata rhizomes. LPFs changed the accumulation of phenolics in H. cordata. The results provide that colonization of LPFs in rhizomes was regulated by the colonizing microbiota of H. cordata, the secondary metabolites in the H. cordata rhizomes, and the mutual inhibition and competition between the different latent pathogens.

Latent Pathogenic Fungi in the Medicinal Plant <italic>Houttuynia cordata</italic> Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

Polish Journal of Microbiology

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Latent Pathogenic Fungi in the Medicinal Plant  Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

Latent pathogenic fungi (LPFs) affect plant growth, but some of them may stably colonize plants. LPFs were isolated from healthy Houttuynia cordata rhizomes to...

Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

Article Category: original-paper

Published Online: Sep 17, 2021

Page range: 359 - 372

Received: Mar 12, 2021

Accepted: Jul 19, 2021

DOI: https://doi.org/10.33073/pjm-2021-034

Keywords
antifungal activities, latent pathogens, maintaining stable colonization, medicinal plant, phenolic accumulation, rhizome extracts

© 2021 Hai-Tao Ye et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Latent Pathogenic Fungi in the Medicinal Plant Houttuynia cordata Thunb. Are Modulated by Secondary Metabolites and Colonizing Microbiota Originating from Soil

Article Category: original-paper

Published Online: Sep 17, 2021

Page range: 359 - 372

Received: Mar 12, 2021

Accepted: Jul 19, 2021

DOI: https://doi.org/10.33073/pjm-2021-034

Keywordsantifungal activities, latent pathogens, maintaining stable colonization, medicinal plant, phenolic accumulation, rhizome extracts

© 2021 Hai-Tao Ye et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Supplementary Material Details

Keywords
antifungal activities, latent pathogens, maintaining stable colonization, medicinal plant, phenolic accumulation, rhizome extracts