<abstract xmlns="http://www.w3.org/1999/xhtml"><italic>Lactobacillus plantarum</italic> BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of <italic>L. plantarum</italic> BW2013 (a low-dose group of 108 CFU/ml, a medium-dose group of 109 CFU/ml, and a high-dose group of 1010 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of <italic>Bacteroidetes</italic> increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of <italic>Alloprevotella</italic> increased in the low-dose group compared to the control group. The increased abundance of <italic>Ruminococcaceae</italic> and decreased abundance of <italic>Candidatus_Saccharimonas</italic> was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and <italic>Alistipes</italic> and <italic>Candidatus_Saccharimonas</italic> decreased in the high-dose group. These results indicated that <italic>L. plantarum</italic> BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.</abstract>

Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in BALB/c mice by 16S rRNA amplicon sequencing. The mice were randomly allocated to the control group and three treatment groups of L. plantarum BW2013 (a low-dose group of 108 CFU/ml, a medium-dose group of 109 CFU/ml, and a high-dose group of 1010 CFU/ml). The weight of mice was recorded once a week, and the fecal samples were collected for 16S rRNA amplicon sequencing after 28 days of continuous treatment. Compared with the control group, the body weight gain in the treatment groups was not significant. The 16S rRNA amplicon sequencing analysis showed that both the Chao1 and ACE indexes increased slightly in the medium-dose group compared to the control group, but the difference was not significant. Based on PCoA results, there was no significant difference in β diversity between the treatment groups. Compared to the control group, the abundance of Bacteroidetes increased in the low-dose group. The abundance of Firmicutes increased in the medium-dose group. At the genus level, the abundance of Alloprevotella increased in the low-dose group compared to the control group. The increased abundance of Ruminococcaceae and decreased abundance of Candidatus_Saccharimonas was observed in the medium-dose group. Additionally, the abundance of Bacteroides increased, and Alistipes and Candidatus_Saccharimonas decreased in the high-dose group. These results indicated that L. plantarum BW2013 could ameliorate gut microbiota composition, but its effects vary with the dose.

The Effect of <italic>Lactobacillus plantarum</italic> BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Effect of  BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

Lactobacillus plantarum BW2013 was isolated from the fermented Chinese cabbage. This study aimed to test the effect of this strain on the gut microbiota in...

Group	Observed species	Shannon	Simpson	Chao1	ACE	Goods coverage
IS	532	6.566	0.971	534.371	540.911	0.999
NC	535.6	6.433	0.968	538.985	545.437	0.999
LDG	559	6.622	0.976	563.100	570.366	0.999
MDG	580	6.728	0.978	582.440	588.671	0.999
HDG	584.8	6.640	0.971	616.129	619.041	0.999

The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

Article Category: original-paper

Published Online: Dec 06, 2021

Page range: 235 - 243

Received: Mar 21, 2021

Accepted: May 17, 2021

DOI: https://doi.org/10.33073/pjm-2021-022

Keywords
composition, gut microbiota, 16S rRNA amplicon sequencing, BALB/c mice

© 2021 Tong Tong et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

The α diversity index of gut microbiota in each group.

The Effect of Lactobacillus plantarum BW2013 on The Gut Microbiota in Mice Analyzed by 16S rRNA Amplicon Sequencing

Article Category: original-paper

Published Online: Dec 06, 2021

Page range: 235 - 243

Received: Mar 21, 2021

Accepted: May 17, 2021

DOI: https://doi.org/10.33073/pjm-2021-022

Keywordscomposition, gut microbiota, 16S rRNA amplicon sequencing, BALB/c mice

© 2021 Tong Tong et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

The α diversity index of gut microbiota in each group.

Keywords
composition, gut microbiota, 16S rRNA amplicon sequencing, BALB/c mice