<abstract xml:lang="en" xmlns="http://www.w3.org/1999/xhtml"><p>A new RT-PCR primer pair designed to identify Acute Bee Paralysis Virus (ABPV), Kashmir Bee Virus (KBV) or Israeli Acute Bee Paralysis Virus (IAPV) of honey bees (<italic>Apis mellifera</italic> L.) in a single assay is described. These primers are used to screen samples for ABPV, KBV, or IAPV in a single RT-PCR reaction saving time and money. The primers are located in the predicted overlapping gene (<italic>pog</italic>/ORFX) which is highly conserved across ABPV, KBV, IAPV and other dicistroviruses of social insects. This study has also identified the first case of IAPV in Denmark.</p></abstract>

A new RT-PCR primer pair designed to identify Acute Bee Paralysis Virus (ABPV), Kashmir Bee Virus (KBV) or Israeli Acute Bee Paralysis Virus (IAPV) of honey bees (Apis mellifera L.) in a single assay is described. These primers are used to screen samples for ABPV, KBV, or IAPV in a single RT-PCR reaction saving time and money. The primers are located in the predicted overlapping gene (pog/ORFX) which is highly conserved across ABPV, KBV, IAPV and other dicistroviruses of social insects. This study has also identified the first case of IAPV in Denmark.

Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

Department of Integrated Pest Management, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark

Journal of Apicultural Science

A new RT-PCR primer pair designed to identify Acute Bee Paralysis Virus (ABPV), Kashmir Bee Virus (KBV) or Israeli Acute Bee Paralysis Virus (IAPV) of honey...

Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

Published Online: Jun 19, 2012

Page range: 137 - 146

DOI: https://doi.org/10.2478/v10289-012-0014-x

Keywords
honey bee virus, IAPV, KBV, ABPV, AKI primers

This content is open access.

Single Assay Detection of Acute Bee Paralysis Virus, Kashmir Bee Virus and Israeli Acute Paralysis Virus

Published Online: Jun 19, 2012

Page range: 137 - 146

DOI: https://doi.org/10.2478/v10289-012-0014-x

Keywordshoney bee virus, IAPV, KBV, ABPV, AKI primers

This content is open access.

Keywords
honey bee virus, IAPV, KBV, ABPV, AKI primers