<abstract xmlns="http://www.w3.org/1999/xhtml"> Objectives: There are many treatment protocols used these days, but seems like no one ensures a hundred percent effectiveness for lifelong prosthesis function. The aim of the study was to investigate whether some new method could mitigate calcification better than usually used and to compare impact on different valve tissues.Methods: Porcine aortic and mitral valve cusps were treated with glutaraldehyde and carbodiimide. Control group without any treatment was also used. Cusp pieces were implanted subcutaneously into 90 rats for 60 days. Tissue calcification was assessed by atomic absorption spectrophotometry.Results: The most calcification was found when not used any treatment, and the lowest calcium level was in group when treated with carbodiimide. Glutaraldehyde in low concentration lowered significantly residual calcium level, but not so effectively as carbodiimide. All data differ statistically significantly (p &lt; 0.05).Conclusion: Calcification was minimal treating porcine valve tissue with carbodiimide comparing to glutaraldehyde or no treatment.</abstract>

Objectives: There are many treatment protocols used these days, but seems like no one ensures a hundred percent effectiveness for lifelong prosthesis function. The aim of the study was to investigate whether some new method could mitigate calcification better than usually used and to compare impact on different valve tissues.Methods: Porcine aortic and mitral valve cusps were treated with glutaraldehyde and carbodiimide. Control group without any treatment was also used. Cusp pieces were implanted subcutaneously into 90 rats for 60 days. Tissue calcification was assessed by atomic absorption spectrophotometry.Results: The most calcification was found when not used any treatment, and the lowest calcium level was in group when treated with carbodiimide. Glutaraldehyde in low concentration lowered significantly residual calcium level, but not so effectively as carbodiimide. All data differ statistically significantly (p < 0.05).Conclusion: Calcification was minimal treating porcine valve tissue with carbodiimide comparing to glutaraldehyde or no treatment.

Impact on porcine heart valve tissue treating with carbodiimide

Department of Cardiovascular Diseases, Vilnius University, Vilnius, Lithuania

Department of Cardiac Surgery, Seamen’s Hospital, Liepojos 45, LT-92288, Klaipeda, Lithuania. Tel.: +370 650 12900; Fax: +370 46385338

Faculty of Chemistry, Vilnius University, Vilnius, Lithuania

Seminars in Cardiovascular Medicine

{"article-title":"Impact on porcine heart valve tissue treating with carbodiimide"}

Objectives: There are many treatment protocols used these days, but seems like no one ensures a hundred percent effectiveness for lifelong prosthesis function....

Impact on porcine heart valve tissue treating with carbodiimide

Published Online: Feb 16, 2013

Page range: 1 - 3

DOI: https://doi.org/10.2478/v10287-012-0006-0

Keywords
glutaraldehyde, carbodiimide, mitral and aortic bioprosthesis calcification

This content is open access.

Impact on porcine heart valve tissue treating with carbodiimide

Published Online: Feb 16, 2013

Page range: 1 - 3

DOI: https://doi.org/10.2478/v10287-012-0006-0

Keywordsglutaraldehyde, carbodiimide, mitral and aortic bioprosthesis calcification

This content is open access.

Keywords
glutaraldehyde, carbodiimide, mitral and aortic bioprosthesis calcification